Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro
eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Transgenic Targeting of Fcrls Creates a Highly Efficient Constitutively Active Microglia Cre Line with Differentiated Specificity

Tobias Kaiser, Jordan Dattero, Liang Li, Mandy Chen, Minqing Jiang, Andrew Harrahill, Oleg Butovsky and Guoping Feng
eNeuro 26 June 2024, 11 (7) ENEURO.0549-23.2024; https://doi.org/10.1523/ENEURO.0549-23.2024
Tobias Kaiser
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jordan Dattero
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
3Bioengineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liang Li
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mandy Chen
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Minqing Jiang
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew Harrahill
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Oleg Butovsky
4Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115
5Evergrande Center for Immunologic Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guoping Feng
1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
6Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Guoping Feng
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Information

DOI 
https://doi.org/10.1523/ENEURO.0549-23.2024
PubMed 
38926085
Published By 
Society for Neuroscience
History 
  • Received December 26, 2023
  • Revision received April 27, 2024
  • Accepted May 13, 2024
  • Published online June 26, 2024.
Copyright & Usage 
Copyright © 2024 Kaiser et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Tobias Kaiser1,2,
  2. Jordan Dattero1,3,
  3. Liang Li1,2,
  4. Mandy Chen1,2,
  5. Minqing Jiang1,2,
  6. Andrew Harrahill1,2,
  7. Oleg Butovsky4,5 and
  8. Guoping Feng1,2,6
  1. 1McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts 02139
  2. 2Departments of Brain and Cognitive Sciences, Cambridge, Massachusetts 02139
  3. 3Bioengineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
  4. 4Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115
  5. 5Evergrande Center for Immunologic Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115
  6. 6Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142
  1. Correspondence should be addressed to Tobias Kaiser at tkaiser{at}mit.edu or Guoping Feng at fengg{at}mit.edu.
View Full Text

Author contributions

  1. Author contributions: T.K. and G.F. designed research; T.K., J.D., L.L., M.C., M.J., and A.H. performed research; O.B. contributed reagents/analytic tools; T.K., J.D., L.L., and M.C. analyzed data; T.K. and G.F. wrote the paper with inputs from all authors.

Disclosures

  • The authors declare no competing financial interests.

  • We thank all members of the Feng lab for helpful discussions. We thank Ubadah Sabbagh for comments on the manuscript. We also acknowledge Nicholas Sanders, Morgan Fleishman, Heather Zaniewski, and Dongqing Wang for excellent technical support. T.K. was supported by the Tan-Yang center for Autism Research at MIT. G.F. is supported by the Poitras Center for Affective Disorders Research at MIT, Tan-Yang Center for Autism Research at MIT, Stanley Center for Psychiatric Research at Broad Institute of MIT and Harvard, Nancy Lurie Marks Family Foundation, Simons Foundation Autism Research Initiative (SFARI), and Simons Center for the Social Brain at MIT. This work was also partially supported by Cancer Center Support (core) Grant P30-CA14051 from the NCI.

  • Tan-Yang Center for Autism Research at MIT. Simons Foundation Autism Research Initiative. Simons Center for the Social Brain at MIT. Poitras Center for Affective Disorders Research at MIT. Stanley Center for Psychiatric Research at Broad Institute of MIT and Harvard. Nancy Lurie Marks Family Foundation.

Funding

  • Tan-Yang Center for Autism Research at MIT

Other Version

  • You are viewing the most recent version of this article.
  • previous version (June 26, 2024).
  • previous version (June 26, 2024).

Online Impact

 

Article usage

Select a custom date range for the past year
E.g., 2025-07-19
to
E.g., 2025-07-19

Article usage: June 2024 to July 2025

AbstractFullPdf
Jun 2024142057
Jul 2024363381265
Aug 20243521193
Sep 202431208180
Oct 20242418380
Nov 20241919065
Dec 20241515568
Total 20246291328808
Jan 20252118778
Feb 20251215657
Mar 20251119445
Apr 2025518054
May 20251016346
Jun 20251515540
Jul 202598948
Total 2025831124368
Total71224521176
Back to top

In this issue

eneuro: 11 (7)
eNeuro
Vol. 11, Issue 7
July 2024
  • Table of Contents
  • Index by author
  • Masthead (PDF)
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Transgenic Targeting of Fcrls Creates a Highly Efficient Constitutively Active Microglia Cre Line with Differentiated Specificity
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Transgenic Targeting of Fcrls Creates a Highly Efficient Constitutively Active Microglia Cre Line with Differentiated Specificity
Tobias Kaiser, Jordan Dattero, Liang Li, Mandy Chen, Minqing Jiang, Andrew Harrahill, Oleg Butovsky, Guoping Feng
eNeuro 26 June 2024, 11 (7) ENEURO.0549-23.2024; DOI: 10.1523/ENEURO.0549-23.2024

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Transgenic Targeting of Fcrls Creates a Highly Efficient Constitutively Active Microglia Cre Line with Differentiated Specificity
Tobias Kaiser, Jordan Dattero, Liang Li, Mandy Chen, Minqing Jiang, Andrew Harrahill, Oleg Butovsky, Guoping Feng
eNeuro 26 June 2024, 11 (7) ENEURO.0549-23.2024; DOI: 10.1523/ENEURO.0549-23.2024
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • BAM
  • cre
  • CreERT2
  • Fcrls-Cre
  • microglia
  • neuroimmune

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Article: Methods/New Tools

  • CalTrig: A GUI-Based Machine Learning Approach for Decoding Neuronal Calcium Transients in Freely Moving Rodents
  • Spiking neural network models of interaural time difference extraction via a massively collaborative process
  • Adapt-A-Maze: An Open-Source Adaptable and Automated Rodent Behavior Maze System
Show more Research Article: Methods/New Tools

Novel Tools and Methods

  • CalTrig: A GUI-Based Machine Learning Approach for Decoding Neuronal Calcium Transients in Freely Moving Rodents
  • Spiking neural network models of interaural time difference extraction via a massively collaborative process
  • Adapt-A-Maze: An Open-Source Adaptable and Automated Rodent Behavior Maze System
Show more Novel Tools and Methods

Subjects

  • Novel Tools and Methods
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.