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Research ArticleResearch Article: New Research, Development

Acetylcholine Neurons Become Cholinergic during Three Time Windows in the Developing Mouse Brain

Rene Oliver Goral, Patricia W. Lamb and Jerrel L. Yakel
eNeuro 28 June 2024, 11 (7) ENEURO.0542-23.2024; https://doi.org/10.1523/ENEURO.0542-23.2024
Rene Oliver Goral
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709
2Center on Compulsive Behaviors, National Institutes of Health, Bethesda, Maryland 20892
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Patricia W. Lamb
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709
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Jerrel L. Yakel
1Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0542-23.2024
PubMed 
38942474
Published By 
Society for Neuroscience
History 
  • Received December 19, 2023
  • Revision received June 12, 2024
  • Accepted June 22, 2024
  • Published online June 28, 2024.
Copyright & Usage 
Copyright © 2024 Oliver Goral et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Rene Oliver Goral1,2,
  2. Patricia W. Lamb1 and
  3. Jerrel L. Yakel1
  1. 1Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709
  2. 2Center on Compulsive Behaviors, National Institutes of Health, Bethesda, Maryland 20892
  1. Correspondence should be addressed to Jerrel L. Yakel at yakel{at}niehs.nih.gov or R. Oliver Goral at rene.goral{at}nih.gov.
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Author contributions

  1. Author contributions: R.O.G. and J.L.Y. designed research; R.O.G. and P.W.L. performed research; R.O.G. and P.W.L. analyzed data; R.O.G. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • We thank Scotty D. Dowdy, Maria G. Barrientos Sandoval, and Katrina Loper for assistance with animal husbandry/feeding/care. We thank Erica Scappini, Robert N. Wine, and Charles J. Tucker for technical assistance. All imaging was performed with hardware provided by the NIEHS Fluorescence Microscopy & Imaging Center. We thank Drs. Shiyi Wang, Lorna W. Role, Patricia Jensen, Nicholas W. Plummer, Zhiyong Liu, Joanne C. Damborsky, and members of the Yakel lab for valuable discussions or comments on the experiments or the manuscript. This research was supported by the Intramural Research Program of the National Institutes of Health (NIH, Z01ES090089 to J.L.Y.) and by the Center on Compulsive Behaviors, NIH via NIH Director’s Challenge Award (to R.O.G.).

Funding

  • HHS | National Institutes of Health (NIH)

    Z01ES090089
  • Center on Compulsive Behaviors, NIH via NIH Director's Challenge Award

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Jun 202492035
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eneuro: 11 (7)
eNeuro
Vol. 11, Issue 7
July 2024
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Acetylcholine Neurons Become Cholinergic during Three Time Windows in the Developing Mouse Brain
Rene Oliver Goral, Patricia W. Lamb, Jerrel L. Yakel
eNeuro 28 June 2024, 11 (7) ENEURO.0542-23.2024; DOI: 10.1523/ENEURO.0542-23.2024

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Acetylcholine Neurons Become Cholinergic during Three Time Windows in the Developing Mouse Brain
Rene Oliver Goral, Patricia W. Lamb, Jerrel L. Yakel
eNeuro 28 June 2024, 11 (7) ENEURO.0542-23.2024; DOI: 10.1523/ENEURO.0542-23.2024
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Keywords

  • brain development
  • cholinergic neurons
  • transgenic mice
  • whole-brain imaging

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