Article Figures & Data
Figures
- Figure 1.
Schematic of radixin knockdown. Diagram of an inhibitory synapse and surrounding extrasynaptic area under (A) baseline conditions and (B) when radixin is knocked down. Figure made using BioRender.
- Figure 2.
GL-II-73 was unable to restore dopamine system function when radixin was knocked down. In vivo extracellular electrophysiology was used to measure dopamine cell activity in the VTA. A, left, IS exposure significantly increased the number of spontaneously active cells/track (population activity), which was reversed by intra-ventral hippocampus injection of GL-II-73 (100 ng/μl; 0.75 μl), but not when radixin was knocked down. Right, Representative brain slice with electrode placement in the VTA (black arrow) and cannula placement for drug administration in the vHipp (white arrow), with the corresponding schematics of the brain section (−5.40 mm posterior to bregma) with the box indicating the area in which tracks were found. Neither (B) firing rate nor (C) burst firing was affected by siRNA, shock, or drug treatment. D, Representative traces from control (left) and shocked (right) rats. n = 6–8/group, males and females represented as circles and squares, respectively, ***p = 0.0001, ****p < 0.0001; RDX, radixin.
- Figure 3.
Radixin knockdown does not alter PPI. Two days of IS had a significant main effect on PPI as did treatment with GL-II-73 (p = 0.0042); however, post hoc analysis revealed no relevant group differences. n = 9–11/group, males and females represented as circles and squares, respectively. **P < 0.005; RDX, radixin.
- Figure 4.
Radixin knockdown increases synaptic α5, without changing surface or total α5 expression. A, Coimmunoprecipitation of α5 and radixin revealed a significant decrease in α5-associated radixin in rats that received radixin-targeted siRNA compared with those that received scrambled siRNA. Representative image of bands below. n = 10/group. B, Conversely, coimmunoprecipitation of α5 and gephyrin revealed a significant increase in gephyrin levels in the radixin knockdown group. Representative image of bands below. n = 9–11/group. C, Treatment with the cross-linking agent caused a significant decrease in the optical density of α5 immunoreactive bands, but no differences in total α5 (homogenate) or surface (cross-linked) were observed between rats that received scrambled siRNA or radixin-targeted siRNA. Representative image of bands below graphs. n = 5/group, males and females represented as circles and squares, respectively. *p < 0.05, **p < 0.01 n = 10; RDX, radixin; OD, optical density.
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