Article Figures & Data
Figures
- Figure 1.
hm2α-SYN-39 transgenic mouse overexpress human synuclein. Slide scanning images (20× objective) of a coronal midbrain section through the substantia nigra stained for TH in green in hm2α-SYN-39 transgenic mice and wild-type (WT) mice at (A) low power (20× objective). B, Subsections from A outlined in white stained for TH in green. C, Same sections as in B stained for human synuclein in red, and (D) merged images with co-stained neurons in yellow. E, Confocal images (40× objective, 8× digital zoom) of synuclein expression in dopaminergic neurons of the substantia nigra from a wild-type mouse and an hm2α-SYN-39 transgenic mouse. Scale bars: A–D, 125 μm; E, 5 μm. F, Western blots from two hm2α-SYN-39 transgenic mice and two WT littermate controls.
- Figure 2.
Quantification of TH immunofluorescence in the striatum and substantia nigra is similar in hm2α-SYN-39 and wild-type mice. TH staining (red) from the striatum of (A) wild-type (WT) and (B) hm2α-SYN-39 mice. C, Quantification of TH intensity revealed no consistent differences between genotypes. Similarly, TH from the midbrain of (D) WT and (E) hm2α-SYN-39 mouse. F, Quantification of TH levels revealed no consistent differences between genotypes. Scale bars: A, B and D, E, 1,000 μm. Data from 9 wild-type mice (WT; blue) versus 18 hm2α-SYN-39 mice (red).
- Figure 3.
hm2α-SYN-39 transgenic mice do not have reliable behavioral deficits. A, Time on the rotarod and (B) maximum revolutions per minute (rpm). There were no reliable effects of genotype for 9 wild-type mice (WT; blue) versus 20 hm2α-SYN-39 mice (red). C, Open-field distance traveled for WT versus hm2α-SYN-39 mice; there was a main effect of time, genotype, and an interaction due to the hm2α-SYN-39 mice being less active early and hyperactive late in their lives. Points were jittered for visualization.
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