Article Figures & Data
Figures
- Figure 1.
The materials required to build the multielectrode array.
- Figure 2.
Schematic showing bottom and top plastic pieces of the multielectrode array. A, Schematic showing the bottom plastic piece with cannula holes indicating coordinates for different brain regions. Three brain regions are targeted here—the prefrontal cortex (red dots), cingulate cortex (black dots), and dorsal hippocampus (blue dots). B, Schematic showing the top plastic piece with color-coded pinholes corresponding to electrodes from the bottom plastic piece. G, Pinhole for the ground electrode; R, pinhole for the reference electrode; Amg, amygdala; dStr, dorsal striatum; GP, globus pallidus; Ins, insula; NAc, nucleus accumbens; thal, thalamus; VP, ventral pallidum.
- Figure 3.
Steps for building the multielectrode array. A–J, Bottom (A) and top (B) plastic pieces were joined using a dowel (C, D, E). A cannula was cut to the appropriate length, and a precut wire was inserted through (F). All cannulas with precut wires were threaded through the corresponding holes in the top and bottom plastic pieces (G, H). Pins were inserted into the holes in the top plastic piece to stabilize the wires and to create a connection between the wires and the pins (the insulation is sheared by the pins to allow connection; I). After insertion of all pins (J), extra wires protruding from the top plastic piece were cut, and those from the cannulas were shortened to a predetermined length (according to the desired DV coordinates). The reference and ground wires were left intact. Inset (J), The top of the top plastic piece on completion of the multielectrode array.
- Figure 4.
Stereotaxic surgery to implant multielectrode array. A, Multielectrode array connected to the arm of the stereotaxic frame. B, Cleaned skull showing lambda and bregma. C, Holes drilled in the skull of the rat corresponding to coordinates of targeted brain regions. D, Screws (ground, reference, and anchor) secured into the skull. E, Multielectrode array inserted into the predrilled holes in the skull of the rat. F, Dental cement was applied to the skull to secure the implanted multielectrode array on the head of the rat.
- Figure 5.
A, B, Rat connected to the wired head stage for the Tucker Davies Technology RZ10 system (A) and wireless head stage for the W2100 system (B).
- Figure 6.
Representative traces of local field potential recordings from the left and right dorsal hippocampus, cingulate cortex, and prefrontal cortex.
Tables
- Table 1
Bill for materials for the components of the complete customized multielectrode array
Item Product number Vendor Quantity Cost per CMEA Delrin plastic pieces Amazon 1 (top and bottom pieces) 3.33 Plastic dowel 2500470500 DigiKey 1 0.22 Polyimid tubing 95820-02 VWR International 1 (<35 mm) 1.82 PFA-coated stainless steel wire 791600 A-M Systems 1 (18 inches) 2.93 Mill-Max connector 853–93-100-10-001000 DigiKey 1 (2 × 9 pins) 4.04 Superglue 855977000078 Amazon 1 (100 µl) 0.05 Male/male zero insertion force Zif-Clip
to Mill-Max adaptor8501005010001000 DigiKey 1 (2 × 9 pins) 2.5 Total cost $15.91 Canadian
Table 1-1
Alternative supplier list. Download Table 1-1, DOCX.
Extended Data 1
Sample wireframe IGES files for the Delrin templates (see DOI 10.17605/OSF.IO/ZXF5T). Download Extended Data 1, ZIP file.
In this issue
