Fig. 2 | I | Rostral: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.92806, p-value = 0.5652 heterozygote: W = 0.79679, p-value = 0.05498 | Equal variance F test p-value = 0.3117 | Unpaired Student’s t test | 12.43746 to 40.41903 | 0.002 |
Fig. 2 | I | Middle: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.92585, p-value = 0.5484 heterozygote: W = 0.94264, p-value = 0.6805 | Equal variance F test p-value = 0.4658 | Unpaired Student’s t test | 10.76698 to 42.59023 | 0.004 |
Fig. 2 | I | Caudal: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.956, p-value = 0.7885 heterozygote: W = 0.90402, p-value = 0.3982 | Equal variance F test p-value = 0.6094 | Unpaired Student’s t test | 13.47947 to 41.17302 | 0.001 |
Fig. 2 | J | Rostral: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.93972, p-value = 0.657 heterozygote: W = 0.92768, p-value = 0.5623 | Equal variance F test p-value = 0.178 | Unpaired Student’s t test | 1.766226 to 5.743795 | 0.002 |
Fig. 2 | J | Middle: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.97022, p-value = 0.8939 heterozygote: W = 0.98581, p-value = 0.9765 | Equal variance F test p-value = 0.3674 | Unpaired Student’s t test | 1.605875 to 5.699706 | 0.003 |
Fig. 2 | J | Caudal: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.97971, p-value = 0.9501 heterozygote: W = 0.85301, p-value = 0.1664 | Equal variance F test p-value = 0.658 | Unpaired Student’s t test | 2.105032 to 6.101853 | 0.001 |
Fig. 2 | K | Rostral: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.96263, p-value = 0.8398 heterozygote: W = 0.95556, p-value = 0.7849 | Equal variance F test p-value = 0.3327 | Unpaired Student’s t test | −203.52664 to 24.79879 | 0.112 |
Fig. 2 | K | Middle: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.90479, p-value = 0.403 heterozygote: W = 0.91236, p-value = 0.4521 | Equal variance F test p-value = 0.9113 | Unpaired Student’s t test | −120.71312 to 31.80191 | 0.223 |
Fig. 2 | K | Caudal: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.8622, p-value = 0.1969 heterozygote: W = 0.89865, p-value = 0.366 | Equal variance F test p-value = 0.2966 | Unpaired Student’s t test | −114.245014 to 4.374468 | 0.066 |
Fig. 3 | C | TBR1 (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.87838, p-value = 0.3197 heterozygote: W = 0.99953, p-value = 0.9585 | Unequal variance F test p-value = 0.03097 | Unpaired Welch’s t test | −0.7341526 to 0.7142779 | 0.959 |
Fig. 3 | C | TBR1 (middle): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.95681, p-value = 0.6002 heterozygote: W = 0.89516, p-value = 0.3703 | Equal variance F test p-value = 0.8262 | Unpaired Student’s t test | −0.7341526 to 0.7142779 | 0.543 |
Fig. 3 | C | TBR1 (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.92614, p-value = 0.4743 heterozygote: W = 0.85654, p-value = 0.2581 | Equal variance F test p-value = 0.7396 | Unpaired Student’s t test | −0.7341526 to 0.7142779 | 0.061 |
Fig. 3 | C | RORb (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.83679, p-value = 0.2057 heterozygote: W = 0.91815, p-value = 0.4458 | Equal variance F test p-value = 0.1755 | Unpaired Student’s t test | −0.7304762 to 0.8176759 | 0.845 |
Fig. 3 | C | RORb (middle): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.81895, p-value = 0.1606 heterozygote: W = 0.92359, p-value = 0.4651 | Equal variance F test p-value = 0.666 | Unpaired Student’s t test | −0.7304762 to 0.8176759 | 0.870 |
Fig. 3 | C | RORB (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.85562, p-value = 0.2556 heterozygote: W = 0.98158, p-value = 0.74 | Equal variance F test p-value = 0.07155 | Unpaired Student’s t test | −2.202504 to 1.445244 | 0.486 |
Fig. 3 | C | BRN2 (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.99286, p-value = 0.8385 heterozygote: W = 0.98261, p-value = 0.7474 | Equal variance F test p-value = 0.7735 | Unpaired Student’s t test | −1.8201310 to 0.7466688 | 0.305 |
Fig. 3 | C | BRN2: (middle) wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.99043, p-value = 0.8129 heterozygote: W = 0.76126, p-value = 0.02503 | Equal variance F test p-value = 0.4921 | Mann–Whitney U test | Z = 0.65465 | 0.700 |
Fig. 3 | C | BRN2 (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.984, p-value = 0.7578 heterozygote: W = 0.93876, p-value = 0.5224 | Equal variance F test p-value = 0.3267 | Unpaired Student’s t test | −2.011466 to 1.115294 | 0.413 |
Fig. 3 | D | TBR1 (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.99311, p-value = 0.8413 heterozygote: W = 0.8151, p-value = 0.1511 | Equal variance F test p-value = 0.5543 | Unpaired Welch’s t test | −42.65128 to 80.04344 | 0.445 |
Fig. 3 | D | TBR1 (middle): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.81232, p-value = 0.1443 heterozygote: W = 0.99621, p-value = 0.8824 | Equal variance F test p-value = 0.9107 | Unpaired Student’s t test | −2.011466 to 1.115294 | 0.215 |
Fig. 3 | D | TBR1 (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.99479, p-value = 0.862 heterozygote: W = 0.90284, p-value = 0.3946 | Equal variance F test p-value = 0.362 | Unpaired Student’s t test | −77.58617 to 17.77541 | 0.157 |
Fig. 3 | D | RORb (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.92163, p-value = 0.4581 heterozygote: W = 0.94029, p-value = 0.5286 | Equal variance F test p-value = 0.2323 | Unpaired Student’s t test | −101.65007 to 82.43556 | 0.786 |
Fig. 3 | D | RORb (middle): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.8246, p-value = 0.1747 heterozygote: W = 0.95008, p-value = 0.5697 | Equal variance F test p-value = 0.8781 | Unpaired Student’s t test | −95.84147 to 113.25092 | 0.829 |
Fig. 3 | D | RORb (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.96848, p-value = 0.6591 heterozygote: W = 0.96668, p-value = 0.6494 | Equal variance F test p-value = 0.9331 | Unpaired Student’s t test | −146.25097 to 33.68333 | 0.157 |
Fig. 3 | D | BRN2 (rostral): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.85, p-value = 0.2404 heterozygote: W = 0.88077, p-value = 0.3267 | Equal variance F test p-value = 0.9132 | Unpaired Student’s t test | −259.03986 to 83.37571 | 0.228 |
Fig. 3 | D | BRN2: (middle) wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.90474, p-value = 0.4008 heterozygote: W = 0.9984, p-value = 0.9235 | Equal variance F test p-value = 0.6548 | Unpaired Student’s t test | −211.6771 to 138.7905 | 0.595 |
Fig. 3 | D | BRN2 (caudal): wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98321, p-value = 0.7518 heterozygote: W = 0.95459, p-value = 0.5899 | Equal variance F test p-value = 0.7567 | Unpaired Student’s t test | −259.03986 to 83.37571 | 0.228 |
Fig. 4 | B | Rostral: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.88946, p-value = 0.3527 heterozygote: W = 0.90024, p-value = 0.3863 | Equal variance F test p-value = 0.3471 | Unpaired Student’s t test | −0.5520192 to 0.2715238 | 0.398 |
Fig. 4 | B | Middle: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.97423, p-value = 0.6921 heterozygote: W = 0.77489, p-value = 0.05587 | Equal variance F test p-value = 0.8878 | Unpaired Student’s t test | −0.9814349 to 0.2605900 | 0.182 |
Fig. 4 | B | Caudal: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.96518, p-value = 0.6415 heterozygote: W = 0.94166, p-value = 0.5341 | Equal variance F test p-value = 0.1779 | Unpaired Student’s t test | −1.381734 to 0.107321 | 0.076 |
Fig. 4 | C | Rostral: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98083, p-value = 0.7347 heterozygote: W = 0.99765, p-value = 0.9075 | Equal variance F test p-value = 0.9134 | Unpaired Student’s t test | −334.0189 to 298.0080 | 0.882 |
Fig. 4 | C | Middle: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.83705, p-value = 0.2064 heterozygote: W = 0.95242, p-value = 0.5801 | Equal variance F test p-value = 0.6733 | Unpaired Student’s t test | −157.30316 to 97.03834 | 0.547 |
Fig. 4 | C | Caudal: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.97936, p-value = 0.7246 heterozygote: W = 0.99965, p-value = 0.9645 | Equal variance F test p-value = 0.9995 | Unpaired Student’s t test | −61.38874 to 29.16489 | 0.379 |
Fig. 5 | B | E12.5 BrdU -> P9 fix wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.84955, p-value = 0.2247 heterozygote: W = 0.90757, p-value = 0.4696 | Equal variance F test p-value = 0.06273 | Unpaired Student’s t test | −12.9173912 to 0.5582761 | 0.066 |
Fig. 5 | B | E14.5 BrdU -> P9 fix wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.78785, p-value = 0.08216 heterozygote: W = 0.89427, p-value = 0.4031 | Unequal variance F test p-value = 0.03346 | Unpaired Welch’s t test | −46.46305 to −18.39430 | 0.004 |
Fig. 5 | B | E16.5 BrdU -> P9 fix wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.81206, p-value = 0.1257 heterozygote: W = 0.96273, p-value = 0.7961 | Equal variance F test p-value = 0.6301 | Unpaired Student’s t test | −45.14384 to −13.72628 | 0.004 |
Fig. 5 | D | E16.5 BrdU -> P3 fix wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.87183, p-value = 0.3007 heterozygote: W = 0.79291, p-value = 0.09768 | Equal variance F test p-value = 0.7788 | Unpaired Student’s t test | −25.481745 to −5.383604 | 0.013 |
Fig. 6 | F | Wild type vs heterozygote (normal position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.99526, p-value = 0.8685 heterozygote (normal position): W = 0.90932, p-value = 0.4158 | Equal variance Bartlett test p-value = 0.0678 | Tukey–Kramer test | −437.8421 to 150.364845 | 0.398 |
Fig. 6 | F | Wild type vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.99526, p-value = 0.8685 heterozygote (ectopic position): W = 0.82469, p-value = 0.09685 | Equal variance Bartlett test p-value = 0.0678 | Tukey–Kramer test | −514.7175 to −5.315335 | 0.046 |
Fig. 6 | F | Heterozygote (normal position) vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test heterozygote (normal position): W = 0.90932, p-value = 0.4158 heterozygote (ectopic position): W = 0.82469, p-value = 0.09685 | Equal variance Bartlett test p-value = 0.0678 | Tukey–Kramer test | −138.4233 to 370.978869 | 0.443 |
Fig. 6 | G | Wild type vs heterozygote (normal position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.8105, p-value = 0.1398 heterozygote (normal position): W = 0.9134, p-value = 0.4295 | Equal variance Bartlett test p-value = 0.3671 | Tukey–Kramer test | −6.306416 to 2.5699079 | 0.496 |
Fig. 6 | G | Wild type vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.8105, p-value = 0.1398 heterozygote (ectopic position): W = 0.91542, p-value = 0.473 | Equal variance Bartlett test p-value = 0.3671 | Tukey–Kramer test | −6.826894 to 0.8602276 | 0.131 |
Fig. 6 | G | Heterozygote (normal position) vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test heterozygote (normal position): W = 0.9134, p-value = 0.4295 heterozygote (ectopic position): W = 0.91542, p-value = 0.473 | Equal variance Bartlett test p-value = 0.3671 | Tukey–Kramer test | −2.728482 to 4.9586403 | 0.707 |
Fig. 6 | H | Wild type vs heterozygote (normal position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98032, p-value = 0.7312 heterozygote (normal position): W = 0.97948, p-value = 0.7255 | Unequal variance Bartlett test p-value = 0.0005376 | Steel–Dwass test | | 1.000 |
Fig. 6 | H | Wild type vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98032, p-value = 0.7312 heterozygote (ectopic position): W = 0.92588, p-value = 0.5487 | Unequal variance Bartlett test p-value = 0.0005376 | Steel–Dwass test | | 0.256 |
Fig. 6 | H | Heterozygote (normal position) vs heterozygote (ectopic position) | Normal distribution Shapiro–Wilk normality test heterozygote (normal position): W = 0.97948, p-value = 0.7255 heterozygote (ectopic position): W = 0.92588, p-value = 0.5487 | Unequal variance Bartlett test p-value = 0.0005376 | Steel–Dwass test | | 0.256 |
Fig. 7 | E | Wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98136, p-value = 0.7384 heterozygote: W = 0.82619, p-value = 0.1787 | Equal variance F test p-value = 0.1953 | Unpaired Student’s t test | −27.896256 to 6.444708 | 0.158 |
Fig. 7 | F | Wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.98334, p-value = 0.7528 heterozygote: W = 0.79961, p-value = 0.1136 | Equal variance F test p-value = 0.5522 | Unpaired Student’s t test | −18.92532 to 75.37736 | 0.172 |
Fig. 7 | G | L1: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.89302, p-value = 0.3636 heterozygote: W = 0.89602, p-value = 0.3729 | Equal variance F test p-value = 0.9608 | Unpaired Student’s t test | 49.88475 to 142.24042 | 0.004 |
Fig. 7 | G | L2: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.95785, p-value = 0.6051 heterozygote: W = 0.8297, p-value = 0.1876 | Equal variance F test p-value = 0.1826 | Unpaired Student’s t test | −113.18916 to −22.48398 | 0.021 |
Fig. 7 | H | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.76125, p-value = 0.02501 heterozygote: W = 0.77171, p-value = 0.0486 | | Mann–Whitney U test | Z = −0.65465 | 0.700 |
Fig. 7 | I | L1: wild type vs heterozygote | Normal distribution Shapiro–Wilk normality test wild type: W = 0.80732, p-value = 0.1321 heterozygote: W = 0.998, p-value = 0.9147 | Equal variance F test p-value = 0.6222 | Unpaired Student’s t test | 2.056330 to 4.188658 | 0.001 |
Fig. 7 | I | L2: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.80371, p-value = 0.1234 heterozygote: W = 0.96722, p-value = 0.6523 | Equal variance F test p-value = 0.2364 | Unpaired Student’s t test | −6.311426 to −0.491803 | 0.032 |
Extended Data Fig. 3-1 | B | Rostral: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.85766, p-value = 0.1813 heterozygote: W = 0.82895, p-value = 0.1053 | Equal variance F test p-value = 0.7773 | Unpaired Student’s t test | −0.0421572482 to 0.0007444148 | 0.057 |
Extended Data Fig. 3-1 | B | Middle: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.88419, p-value = 0.2889 heterozygote: W = 0.92997, p-value = 0.5799 | Equal variance F test p-value = 0.1479 | Unpaired Student’s t test | −0.033846035 to −0.002103765 | 0.030 |
Extended Data Fig. 3-1 | B | Caudal: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.93585, p-value = 0.6259 heterozygote: W = 0.90844, p-value = 0.4262 | Equal variance F test p-value = 0.9502 | Unpaired Student’s t test | −0.030801905 to −0.009642128 | 0.002 |
Extended Data Fig. 3-1 | C | Rostral: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.90901, p-value = 0.4299 heterozygote: W = 0.93146, p-value = 0.5914 | Unequal variance F test p-value = 0.02958 | Unpaired Welch’s t test | −164.72649 to 42.39315 | 0.199 |
Extended Data Fig. 3-1 | C | Middle: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.93329, p-value = 0.6057 heterozygote: W = 0.97804, p-value = 0.9414 | Equal variance F test p-value = 0.1447 | Unpaired Student’s t test | −187.04365 to −24.62301 | 0.016 |
Extended Data Fig. 3-1 | C | Caudal: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.82064, p-value = 0.08938 heterozygote: W = 0.80381, p-value = 0.06359 | Equal variance F test p-value = 0.6413 | Unpaired Student’s t test | −232.61149 to 44.94482 | 0.163 |
Extended Data Fig. 3-1 | D | Rostral: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.71196, p-value = 0.008242 heterozygote: W = 0.93999, p-value = 0.6591 | | Mann–Whitney U test | Z = −0.64051 | 0.589 |
Extended Data Fig. 3-1 | D | Middle: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.87287, p-value = 0.2379 heterozygote: W = 0.94396, p-value = 0.6912 | Equal variance F test p-value = 0.8054 | Unpaired Student’s t test | −953.6398 to 772.9249 | 0.820 |
Extended Data Fig. 3-1 | D | Caudal: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.89833, p-value = 0.3642 heterozygote: W = 0.68099, p-value = 0.003873 | | Mann–Whitney U test | Z = −0.2445 | 0.853 |
Extended Data Fig. 3-2 | | TBR1: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.93052, p-value = 0.4906 heterozygote: W = 0.8333, p-value = 0.1967 | Equal variance F test p-value = 0.3502 | Unpaired Student’s t test | −50.99473 to 10.79854 | 0.145 |
Extended Data Fig. 3-2 | | RORB: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.77427, p-value = 0.05444 heterozygote: W = 0.90745, p-value = 0.4096 | Equal variance F test p-value = 0.1612 | Unpaired Student’s t test | −38.81443 to −12.39362 | 0.006 |
Extended Data Fig. 3-2 | | Brn2: wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.9922, p-value = 0.8311 heterozygote: W = 0.87014, p-value = 0.2959 | Equal variance F test p-value = 0.7895 | Unpaired Student’s t test | −57.77120 to 86.12194 | 0.609 |
Extended Data Fig. 4-1 | A | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.97442, p-value = 0.9297 heterozygote: W = 0.77181, p-value = 0.009691 | | Mann–Whitney U test | Z = −1.7228 | 0.089 |
Extended Data Fig. 4-1 | B | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.96529, p-value = 0.8517 heterozygote: W = 0.85836, p-value = 0.09203 | Equal variance F test p-value = 0.7867 | Unpaired Student’s t test | −22.37795 to 178.75553 | 0.119 |
Extended Data Fig. 4-1 | C | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.93988, p-value = 0.5806 heterozygote: W = 0.96297, p-value = 0.8289 | Equal variance F test p-value = 0.3549 | Unpaired Student’s t test | −20.29773 to 17.85329 | 0.894 |
Extended Data Fig. 4-1 | D | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.90943, p-value = 0.3119 heterozygote: W = 0.96112, p-value = 0.8099 | Equal variance F test p-value = 0.6479 | Unpaired Student’s t test | −75.85538 to 144.40307 | 0.519 |
Extended Data Fig. 4-1 | E | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.88971, p-value = 0.1982 heterozygote: W = 0.97785, p-value = 0.9523 | Equal variance F test p-value = 0.944 | Unpaired Student’s t test | −47.06784 to 102.62339 | 0.443 |
Extended Data Fig. 4-1 | F | Wild type vs heterozygote | Non-normal distribution Shapiro–Wilk normality test wild type: W = 0.8859, p-value = 0.181 heterozygote: W = 0.86775, p-value = 0.1163 | Equal variance F test p-value = 0.6467 | Unpaired Student’s t test | −244.6362 to 902.2204 | 0.242 |