Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution

Paul K. LaFosse; Zhishang Zhou; Nina G. Friedman; Yanting Deng; Anna J. Li; Bradley Akitake; Mark H. Histed

doi:10.1523/ENEURO.0378-22.2023

Article Information

DOI

https://doi.org/10.1523/ENEURO.0378-22.2023

PubMed

36858826

Published By

Society for Neuroscience

History

Received September 12, 2022
Revision received February 21, 2023
Accepted February 23, 2023
Published online March 1, 2023.

Copyright © 2023 LaFosse et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

Paul K. LaFosse¹,²,³,^*,
Zhishang Zhou¹,^*,
Nina G. Friedman¹,²,³,
Yanting Deng¹,
Anna J. Li¹,⁴,
Bradley Akitake¹ and
Mark H. Histed¹

¹Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
²National Institutes of Health-University of Maryland Graduate Partnerships Program, Bethesda, MD 20892
³Neuroscience and Cognitive Science Program, University of Maryland College Park, College Park, MD 20742
⁴Department of Biological Structure, University of Washington, Seattle, WA 98195

Correspondence should be addressed to Mark H. Histed at mark.histed{at}nih.gov.

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Author contributions

Author contributions: P.K.L., Z.Z., Y.D., A.J.L., B.A., and M.H.H. designed research; P.K.L., Z.Z., Y.D., A.J.L., and B.A. performed research; P.K.L. analyzed data; P.K.L., N.G.F., and M.H.H. wrote the paper.
↵* P.K.L. and Z.Z. contributed equally to this work.

Disclosures

The authors declare no competing financial interests.
This work was supported by the National Institutes of Health BRAIN Initiative, Grant U19NS107464, and the National Institute of Mental Health Intramural Research Program Grant ZIAMH0020967.