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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution

Paul K. LaFosse, Zhishang Zhou, Nina G. Friedman, Yanting Deng, Anna J. Li, Bradley Akitake and Mark H. Histed
eNeuro 1 March 2023, 10 (3) ENEURO.0378-22.2023; DOI: https://doi.org/10.1523/ENEURO.0378-22.2023
Paul K. LaFosse
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
2National Institutes of Health-University of Maryland Graduate Partnerships Program, Bethesda, MD 20892
3Neuroscience and Cognitive Science Program, University of Maryland College Park, College Park, MD 20742
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Zhishang Zhou
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
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Nina G. Friedman
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
2National Institutes of Health-University of Maryland Graduate Partnerships Program, Bethesda, MD 20892
3Neuroscience and Cognitive Science Program, University of Maryland College Park, College Park, MD 20742
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Yanting Deng
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
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  • ORCID record for Yanting Deng
Anna J. Li
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
4Department of Biological Structure, University of Washington, Seattle, WA 98195
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Bradley Akitake
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
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Mark H. Histed
1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0378-22.2023
PubMed 
36858826
Published By 
Society for Neuroscience
History 
  • Received September 12, 2022
  • Revision received February 21, 2023
  • Accepted February 23, 2023
  • Published online March 1, 2023.
Copyright & Usage 
Copyright © 2023 LaFosse et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Paul K. LaFosse1,2,3,*,
  2. Zhishang Zhou1,*,
  3. Nina G. Friedman1,2,3,
  4. Yanting Deng1,
  5. Anna J. Li1,4,
  6. Bradley Akitake1 and
  7. Mark H. Histed1
  1. 1Intramural Program, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892
  2. 2National Institutes of Health-University of Maryland Graduate Partnerships Program, Bethesda, MD 20892
  3. 3Neuroscience and Cognitive Science Program, University of Maryland College Park, College Park, MD 20742
  4. 4Department of Biological Structure, University of Washington, Seattle, WA 98195
  1. Correspondence should be addressed to Mark H. Histed at mark.histed{at}nih.gov.
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Author contributions

  1. Author contributions: P.K.L., Z.Z., Y.D., A.J.L., B.A., and M.H.H. designed research; P.K.L., Z.Z., Y.D., A.J.L., and B.A. performed research; P.K.L. analyzed data; P.K.L., N.G.F., and M.H.H. wrote the paper.

  2. ↵* P.K.L. and Z.Z. contributed equally to this work.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the National Institutes of Health BRAIN Initiative, Grant U19NS107464, and the National Institute of Mental Health Intramural Research Program Grant ZIAMH0020967.

Funding

  • HHS | NIH | National Institute of Mental Health (NIMH)

    ZIAMH0020967
  • HHS | National Institutes of Health (NIH)

    U19NS107464

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Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution
Paul K. LaFosse, Zhishang Zhou, Nina G. Friedman, Yanting Deng, Anna J. Li, Bradley Akitake, Mark H. Histed
eNeuro 1 March 2023, 10 (3) ENEURO.0378-22.2023; DOI: 10.1523/ENEURO.0378-22.2023

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Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution
Paul K. LaFosse, Zhishang Zhou, Nina G. Friedman, Yanting Deng, Anna J. Li, Bradley Akitake, Mark H. Histed
eNeuro 1 March 2023, 10 (3) ENEURO.0378-22.2023; DOI: 10.1523/ENEURO.0378-22.2023
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