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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Meso-Py: Dual Brain Cortical Calcium Imaging in Mice during Head-Fixed Social Stimulus Presentation

Nicholas J. Michelson, Federico Bolaños, Luis A. Bolaños, Matilde Balbi, Jeffrey M. LeDue and Timothy H. Murphy
eNeuro 1 December 2023, 10 (12) ENEURO.0096-23.2023; https://doi.org/10.1523/ENEURO.0096-23.2023
Nicholas J. Michelson
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Federico Bolaños
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Luis A. Bolaños
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Matilde Balbi
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Jeffrey M. LeDue
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Timothy H. Murphy
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0096-23.2023
PubMed 
38053472
Published By 
Society for Neuroscience
History 
  • Received March 20, 2023
  • Revision received November 6, 2023
  • Accepted November 14, 2023
  • Published online December 1, 2023.
Copyright & Usage 
Copyright © 2023 Michelson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Nicholas J. Michelson1,2,
  2. Federico Bolaños1,2,
  3. Luis A. Bolaños1,2,
  4. Matilde Balbi1,2,
  5. Jeffrey M. LeDue1,2 and
  6. Timothy H. Murphy1,2
  1. 1Department of Psychiatry, Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
  2. 2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
  1. Correspondence should be addressed to Timothy H. Murphy at thmurphy{at}mail.ubc.ca.
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Author contributions

  1. Author contributions: N.J.M., F.B., L.A.B., M.B., J.M.L., and T.H.M. designed research; N.J.M., F.B., and L.A.B. performed research; N.J.M., F.B., and L.A.B. analyzed data; N.J.M., L.A.B., and T.H.M. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by Canadian Institutes of Health Research (CIHR) Grants T.H.M. FDN-143209 and PJT-180631, the Brain Canada for the Canadian Neurophotonics Platform, and the Canadian Open Neuroscience Platform initiative. We acknowledge support from an NVIDIA Academic Grant for GPU hardware and a National Science and Engineering Council of Canada (NSERC) Grant GPIN-2022-03723. This work was also supported by computational resources made available through the NeuroImaging and NeuroComputation Centre at the Djavad Mowafaghian Centre for Brain Health Grant RRID SCR_019086 and the Dynamic Brain Circuits in Health and Disease Research Excellence Cluster DataBinge Forum.

Funding

  • Fondation Brain Canada (Brain Canada)

    FDN-143209
  • Neuroimaging and Neurocomputation Centre at the Djavad Mowafaghian Centre for Brain Health

    RRID: SCR_019086

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Meso-Py: Dual Brain Cortical Calcium Imaging in Mice during Head-Fixed Social Stimulus Presentation
Nicholas J. Michelson, Federico Bolaños, Luis A. Bolaños, Matilde Balbi, Jeffrey M. LeDue, Timothy H. Murphy
eNeuro 1 December 2023, 10 (12) ENEURO.0096-23.2023; DOI: 10.1523/ENEURO.0096-23.2023

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Meso-Py: Dual Brain Cortical Calcium Imaging in Mice during Head-Fixed Social Stimulus Presentation
Nicholas J. Michelson, Federico Bolaños, Luis A. Bolaños, Matilde Balbi, Jeffrey M. LeDue, Timothy H. Murphy
eNeuro 1 December 2023, 10 (12) ENEURO.0096-23.2023; DOI: 10.1523/ENEURO.0096-23.2023
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Keywords

  • Cortex
  • GCaMP
  • mesoscale
  • mouse
  • social interaction
  • whisker

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