Figure 1 |
Figure 1B | One -Way Anova Water consumption the before the test Water Saccharin 1x Quinine 1x Saccharin 5x Saccharin 1x (4hrs) | ANOVA results: F = 0.9766 P = 0.4424 R squared, 0.1634 |
Figure 1D | Two-way repeated measures ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons F-I curve Cage control Water Saccharin 1x Quinine 1x Saccharin 5x Saccharin 1x (4hr) | ANOVA Results: Treatment; p = 0.0057, F (5, 110) = 3.491 Current; p < 0.0001, F (8, 880) = 1276 Interaction; p < 0.0001, F (40, 880) = 4.141 Multiple Comparisons: 0pA Cage control vs. Water Mean difference = 0.000 Cage control vs. Saccharin 1x Mean difference = 0.000 Cage control vs. Quinine 1x Mean difference = 0.000 Cage Control vs. Saccharin 5x Mean difference = 0.000 Cage Control vs. Saccharin 1x (4hr) Mean difference = 0.000 Water vs. Saccharin 1x Mean difference = 0.000 Water vs. Quinine 1x Mean difference = 0.000 Water vs. Saccharin 5x Mean difference = 0.000 Water vs. Saccharin 1x (4hr) Mean difference = 0.000 Saccharin 1x vs. Quinine 1x Mean difference = 0.000 Saccharin 1x vs. Saccharin 5x Mean difference = 0.000 Saccharin 1x vs. Saccharin 1x (4hr) Mean difference = 0.000 Quinine 1x vs. Saccharin 5x Mean difference = 0.000 Quinine 1x vs. Saccharin 1x (4hr) Mean difference = 0.000 Saccharin 5x vs. Saccharin 1x (4hr) Mean difference = 0.000 50pA Cage control vs. Water p = 0.9902, q = 0.8645, df = 990.0; Cage control vs. Saccharin 1x p = 0.9993, q = 0.5005, df = 990.0; Cage Control vs. Quinine 1x p >0.9999, q = 0.2597, df = 990.00; Cage control vs. Saccharin 5x p = 0.9451, q = 1.281, df = 990.00; Cage control vs. Saccharin 1x(4hr) p = 0.9922, q = 0.8240, df = 990; Water vs. Saccharin 1x p = 0.9999, q = 0.3522, df = 990 Water vs. Quinine 1x p = 0.9946, q = 0.5927, df = 990; Water vs. Saccharin 5x p = 0.9984, q = 0.4868, df = 990; Water vs. Saccharin 1x (4hr) p>0.9999, q = 0.02212, df = 990; Saccharin 1x vs. Quinine 1x p>0.9999, q = 0.2374, df = 990; Saccharin 1x vs. Saccharin 5x p = 0.9931, q = 0.8029, df = 990; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.9999, q = 0.3478, df = 990; Quinine 1x vs. Saccharin 5x p = 0.9790, q = 1.024, df = 990; Quinine 1x vs. Saccharin 1x (4hr) p = 0.9986, q = 0.5716, df = 990; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.9996, q = 0.4320, df = 990; 100pA Cage control vs. Water p = 0.8652, q = 1.610, df = 990.0; Cage control vs. Saccharin 1x p = 0.2233, q = 3.159, df = 990.0; Cage Control vs. Quinine 1x p = 0.4580, q = 2.563, df = 990.00; Cage control vs. Saccharin 5x p = 0.6454, q = 2.163, df = 990.00; Cage control vs. Saccharin 1x(4hr) p = 0.5930, q = 2.275, df = 990; Water vs. Saccharin 1x p = 0.8437, q = 1.677, df = 990 Water vs. Quinine 1x p = 0.9742, q = 1.073, df = 990; Water vs. Saccharin 5x p = 0.9969, q = 0.6743, df = 990; Water vs. Saccharin 1x (4hr) p = 0.9926, q = 0.8138, df = 990; Saccharin 1x vs. Quinine 1x p = 0.9987, q = 0.5625, df = 990; Saccharin 1x vs. Saccharin 5x p = 0.9867, q = 0.9250, df = 990; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.9945, q = 0.7652, df = 990; Quinine 1x vs. Saccharin 5x p = 0.9998, q = 0.3658, df = 990; Quinine 1x vs. Saccharin 1x (4hr) p>0.9999, q = 0.2164, df = 990; Saccharin 5x vs. Saccharin 1x (4hr) p>0.9999, q = 0.1422, df = 990; 150pA Cage control vs. Water p = 0.8024, q = 1.793, df = 990.0; Cage control vs. Saccharin 1x p = 0.0085, q = 4.836, df = 990.0; Cage Control vs. Quinine 1x p = 0.1482, q = 3.431, df = 990.00; Cage control vs. Saccharin 5x p = 0.2881, q = 2.970, df = 990.00; Cage control vs. Saccharin 1x(4hr) p = 0.5463, q = 2.374, df = 990; Water vs. Saccharin 1x p = 0.1962, q = 3.248, df = 990 Water vs. Quinine 1x p = 0.8009, q = 1.797, df = 990; Water vs. Saccharin 5x p = 0.9345, q = 1.337, df = 990; Water vs. Saccharin 1x (4hr) p = 0.9953, q = 0.7397, df = 990; Saccharin 1x vs. Quinine 1x p = 0.9297, q = 1.361, df = 990; Saccharin 1x vs. Saccharin 5x p = 0.8142, q = 1.762, df = 990; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.5843, q = 2.293, df = 990; Quinine 1x vs. Saccharin 5x p = 0.9997, q = 0.4145, df = 990; Quinine 1x vs. Saccharin 1x (4hr) p = 0.9843, q = 0. 0.9603, df = 990; Saccharin 5x vs. Saccharin 1x (4hr) P = 0.9989, q = 0.5448, df = 990; 200pA Cage Control vs. Water p = 0.9968, q = 0.6822, df = 990.0; Cage Control vs. Saccharin 1x p = 0.0038, q = 0.6822, df = 990.0; Cage Control vs. Quinine 1x p = 0.5360, q = 2.396, df = 990.0; Cage Control vs. Saccharin 5x p = 0.5556, q = 2.354, df = 990.0; Cage Control vs. Saccharin 1x (4hr) p = 0.9876, q = 0.9108, df = 990.0; Water vs. Saccharin 1x p = 0.0116, q = 4.708, df = 990.0; Water vs. Quinine 1x p = 0.7904, q = 4.708, df = 990.0; Water vs. Saccharin 5x p = 0.8042, q = 1.789, df = 990.0; Water vs. Saccharin 1x (4hr) p>0.9999, q = 0.2894, df = 990.0; Saccharin 1x vs. Quinine 1x p = 0.3853, q = 2.727, df = 990.0; Saccharin 1x vs. Saccharin 5x p = 0.3979, q = 2.698, df = 990.0; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.0457, q = 4.083, df = 990.0; Quinine 1x vs. Saccharin 5x p>0.9999, q = 0.008880, df = 990.0; Quinine 1x vs. Saccharin 1x (4hr) p = 0.9172, q = 1.417, df = 990.0; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.9233, q = 1.391, df = 990.0; 250pA Cage control vs. Water p = 0.9988, q = ,0.5590 df = 990.0; Cage control vs. Saccharin 1x p = 0.0011, q = 5.603, df = 990.0; Cage Control vs. Quinine 1x p = 0.7555, q = 1.912, df = 990.0; Cage Control vs. Saccharin 5x p = 0.7478, q = 1.931, df = 990.0; Cage Control vs. Saccharin 1x (4hr) p>0.9999, q = 0.1164, df = 990.0; Water vs. Saccharin 1x p = 0.0026, q = 5.304, df = 990.0; Water vs. Quinine 1x p = 0.9113, q = 1.442, df = 990.0; Water vs. Saccharin 5x p = 0.9051, q = 1.468, df = 990.0; Water vs. Saccharin 1x (4hr) p = 0.9972, q = 0.6633, df = 990.0; Saccharin 1x vs. Quinine 1x p = 0.1000, q = 3.666, df = 990.0; Saccharin 1x vs. Saccharin 5x p = 0.1180, q = 3.570, df = 990.0; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.0013, q = 5.559, df = 990.0; Quinine 1x vs. Saccharin 5x p>0.9999, q = 0.04456, df = 990.0; Quinine 1x vs. Saccharin 1x (4hr) p = 0.7292, q = 1.975, df = 990.0; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.7215, q = 1.993, df = 990.0; 300pA Cage Control vs. Water p = 0.9993, q = 0.4987, df = 990.0; Cage Control vs. Saccharin 1x p = 0.0005, q = 5.903, df = 990.0; Cage Control vs. Quinine 1x p = 0.9022, q = 1.479, df = 990.0; Cage Control vs. Saccharin 5x p = 0.7419, q = 1.945, df = 990.0; Cage Control vs. Saccharin 1x (4hr) p = 0.9641, q = 1.158, df = 990.0; Water vs. Saccharin 1x p = 0.0009, q = 5.679, df = 990.0; Water vs. Quinine 1x p = 0.9766, q = 1.049, df = 990.0; Water vs. Saccharin 5x p = 0.8854, q = 1.542, df = 990.0; Water vs. Saccharin 1x (4hr) p = 0.8386, q = 1.692, df = 990.0; Saccharin 1x vs. Quinine 1x p = 0.0232, q = 4.405, df = 990.0; Saccharin 1x vs. Saccharin 5x p = 0.0716, q = 3.851, df = 990.0; Saccharin 1x vs. Saccharin 1x (4hr) p < 0.0001, q = 6.905, df = 990.0; Quinine 1x vs. Saccharin 5x p = .9994, q = 0.4860, df = 990.0; Quinine 1x vs. Saccharin 1x (4hr) p = 0.4433, q = 2.596, df = 990.0; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.2645, q = 3.035, df = 990.0; 350pA Cage Control vs. Water p = 0.9973, q = 0.6556, df = 990.0; Cage Control vs. Saccharin 1x p = 0.0003, q = 6.069, df = 990.0; Cage Control vs. Quinine 1x p = 0.9972, q = 0.6596, df = 990.0; Cage Control vs. Saccharin 5x p = 0.8294, q = 1.719, df = 990.0; Cage Control vs. Saccharin 1x (4hr) p = 0.7347, q = 1.962, df = 990.0; Water vs. Saccharin 1x p = 0.0009, q = 5.694, df = 990.0; Water vs. Quinine 1x p>0.9999, q = 0.03478, df = 990.0; Water vs. Saccharin 5x p = 0.9650, q = 1.151, df = 990.0; Water vs. Saccharin 1x (4hr) p = 0.4043, q = 2.683, df = 990.0; Saccharin 1x vs. Quinine 1x p = 0.0020, q = 5.401, df = 990.0; Saccharin 1x vs. Saccharin 5x p = 0.0328, q = 4.244, df = 990.0; Saccharin 1x vs. Saccharin 1x (4hr) p < 0.0001, q = 7.879, df = 990.0; Quinine 1x vs. Saccharin 5x p = 0.9747, q = 1.068, df = 990.0; Quinine 1x vs. Saccharin 1x (4hr) p = 0.4400, q = 2.603, df = 990.0; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.1105, q = 3.609, df = 990.0; 400pA Cage Control vs. Water p = 0.9988, q = 0.5513, df = 990.0; Cage Control vs. Saccharin 1x p = 0.0004, q = 5.939, df = 990.0; Cage Control vs. Quinine 1x p = 0.9987, q = 0.5623, df = 990.0; Cage Control vs. Saccharin 5x p = 0.9132, q = 1.435, df = 990.0; Cage Control vs. Saccharin 1x (4hr) p = 0.3765, q = 2.748, df = 990.0; Water vs. Saccharin 1x p = 0.0009, q = 5.663, df = 990.0; Water vs. Quinine 1x p>0.9999, q = 0.03710, df = 990.0; Water vs. Saccharin 5x p = 0.9845, q = 0.9564, df = 990.0; Water vs. Saccharin 1x (4hr) p = 0.1551, q = 3.402, df = 990.0; Saccharin 1x vs. Quinine 1x p = 0.0021, q = 5.369, df = 990.0; Saccharin 1x vs. Saccharin 5x p = 0.0233, q = 4.403, df = 990.0; Saccharin 1x vs. Saccharin 1x (4hr) p < 0.0001, q = 8.548, df = 990.0; Quinine 1x vs. Saccharin 5x p = 0.9894, q = 0.8800, df = 990.0; Quinine 1x vs. Saccharin 1x (4hr) p = 0.1832, q = 3.294, df = 990.0; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.0435, q = 4.108, df = 990.0. |
Figure 1H | One-way ANOVA Kruskal-Wallis test Post-hoc Dunn's multiple comparisons test LIV-VI aIC-BLA neurons fAHP Cage control Water Saccharin 1x Quinine 1x Saccharin 5x Saccharin 1x (4hr) | ANOVA results: Kruskal-Wallis test, p < 0.0001; Kruskal-Wallis statistic, 29.91. Multiple Comparisons: Cage Control vs. Water p >0.9999, z = 0.2306; Cage Control vs. Saccharin 1x p = 0.0136, z = 3.318; Cage Control vs. Quinine 1x p >0.9999, z = 1.809; Cage Control vs. Saccharin 5x p >0.9999, z = 0.4824; Cage Control vs. Saccharin 1x (4hr) p>0.9999, z = 1.648; Water vs. Saccharin 1x p = 0.0177, z = 3.243; Water vs. Quinine 1x p = 0.5054, z = 2.124; Water vs. Saccharin 5x p>0.9999, z = 0.2771; Water vs. Saccharin 1x (4hr) p>0.9999, z = 1.497; Saccharin 1x vs. Quinine 1x p < 0.0001, z = 5.150; Saccharin 1x vs. Saccharin 5x p = 0.0807, z = 2.783; Saccharin 1x vs. Saccharin 1x (4hr) p>0.9999, z = 1.554; Quinine 1x vs. Saccharin 5x p = 0.3511 z = 2.267; Quinine 1x vs. Saccharin 1x (4hr) p = 0.0099, z = 3.406; Saccharin 5x vs. Saccharin 1x (4hr) p>0.9999, z = 1.1583 |
Figure 1I | One-way ANOVA Kruskal-Wallis test Post-hoc Dunn's multiple comparisons test LIV-VI aIC-BLA neurons Action Potential Half-width Cage control Water Saccharin 1x Quinine 1x Saccharin 5x Saccharin 1x (4hr) | ANOVA results: Kruskal-Wallis test, p = 0.0125; Kruskal-Wallis statistic,14.54. Multiple Comparisons: Cage Control vs. Water p >0.9999, z = 0.7692; Cage Control vs. Saccharin 1x p>0.9999, z = 1.627; Cage Control vs. Quinine 1x p >0.9999, z = 0.9868 ; Cage Control vs. Saccharin 5x p >0.9999, z = 0.6540; Cage Control vs. Saccharin 1x (4hr) P = 0.8292, z = 1.917; Water vs. Saccharin 1x p >0.9999, z = 0.9244; Water vs. Quinine 1x p>0.9999, z = 0.2636; Water vs. Saccharin 5x p>0.9999, z = 0.07420; Water vs. Saccharin 1x (4hr) p = 0.0905, z = 2.746; Saccharin 1x vs. Quinine 1x p>0.9999, z = 0.6271 Saccharin 1x vs. Saccharin 5x p = >0.9999, z = 0.9418; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.0065, z = 3.519; Quinine 1x vs. Saccharin 5x p>0.9999z = 0.3194; Quinine 1x vs. Saccharin 1x (4hr) p = 0.0605, z = 2.876; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.1721, z = 2.528 |
Figure 1J | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons Membrane Time Constant Cage control Water Saccharin 1x Quinine 1x Saccharin 5x Saccharin 1x (4hr) | ANOVA results: Treatment; p < 0.0001, F (5, 110) = 6.094; R squared, 0.2169; Multiple Comparisons: Cage Control vs. Water p = 0.4608, q = 2.566, df = 110; Cage Control vs. Saccharin 1x p >0.9999, q = 0.1233, df = 110; Cage Control vs. Quinine 1x p = 0.0864, q = 3.798, df = 110; Cage Control vs. Saccharin 5x p = 0.9398, q = 1.306, df = 110; Cage Control vs. Saccharin 1x (4hr) p = 0.0003, q = 6.326, df = 110; Water vs. Saccharin 1x p = 0.3890, q = 2.731, df = 110; Water vs. Quinine 1x p = 0.9184, q = 1.408, df = 110; Water vs. Saccharin 5x p = 0.9628, q = 1.163, df = 110; Water vs. Saccharin 1x (4hr) p = 0.0488, q = 4.115, df = 110; Saccharin 1x vs. Quinine 1x p = 0.0639, q = 3.969, df = 110; Saccharin 1x vs. Saccharin 5x p = 0.9101, q = 1.443, df = 110; Saccharin 1x vs. Saccharin 1x (4hr) p = 0.0002, q = 6.521, df = 110; Quinine 1x vs. Saccharin 5x p = 0.5180, q = 2.440, df = 110; Quinine 1x vs. Saccharin 1x (4hr) p = 0.4302, q = 2.635, df = 110; Saccharin 5x vs. Saccharin 1x (4hr) p = 0.0081, q = 4.975, df = 110; |
Figure 2 |
Figure 2B | Two-tailed Unpaired t-test Saccharin consumption on the test day Saccharin 2x CTA retrieval | t-test Results: Mann-Whitney test, p = 0.0085, Mann-Whitney U, 2.500 |
Figure 2D | Two-way repeated measures ANOVA Post-hoc Šídák's multiple comparisons test LIV-VI aIC-BLA neurons F-I curve Saccharin 2x CTA retrieval | ANOVA Results: Treatment; p < 0.0014, F (1, 45) = 11.60 Current; p < 0.0001, F (8, 360) = 483.3 Interaction; p < 0.0001, F (8, 360) = 9.398 Multiple Comparisons: 0pA Saccharin 2x vs. CTA retrieval Mean difference = 0.000 50pA Saccharin 2x vs. CTA retrieval p>0.9999, t = 0.1045, df = 405.0; 100pA Saccharin 2x vs. CTA retrieval p = 0.5860, t = 1.682, df = 405.0; 150pA Saccharin 2x vs. CTA retrieval p = 0.0286, t = 2.964, df = 405.0; 200pA Saccharin 2x vs. CTA retrieval p = 0.0019, t = 3.738, df = 405.0; 250pA Saccharin 2x vs. CTA retrieval p = 0.0005, t = 4.090, df = 405.0; 300pA Saccharin 2x vs. CTA retrieval p = 0.0002, t = 4.280, df = 405.0; 350pA Saccharin 2x vs. CTA retrieval p < 0.0001, t = 4.517, df = 405.0; 400pA Saccharin 2x vs. CTA retrieval p = 0.0003, t = 4.161, df = 405.0 |
Figure 2G | Two-tailed Unpaired t-test LIV-VI aIC-BLA neurons Action Potential Amplitude Saccharin 2x CTA retrieval | t-test Results: p = 0.0002 t = 3.983 df = 45 Difference between means = 7.080±1.777 R squared, 0.2607 |
Figure 2H | Two-tailed Unpaired t-test LIV-VI aIC-BLA neurons Input Resistance Saccharin 2x CTA retrieval | t-test Results: p = 0.0036 t = 3.072 df = 45 Difference between means = 44.75±14.57 R squared = 0.1734 |
Figure 2I | Two-tailed Unpaired t-test LIV-VI aIC-BLA neurons SAG Ratio Saccharin 2x CTA retrieval | t-test Results: p = 0.0037 t = 3.060 df = 45 Difference between means = 5.597±1.829 R squared = 0.1723 |
Figure 3 |
Figure 3B | Two-way ANOVA Post-hoc Šídák's multiple comparisons test | ANOVA Results: Treatment, P = 0.068, F (1, 54) = 3.466; Interaction, P = 0.9697, F (8, 54) = 0.2803. |
Figure 3C | Two-tailed Unpaired t-test Saccharin consumption on the test day Extinction Reinstatement | t-test Results: Mann-Whitney test, p = 0.0179; Mann-Whitney U, 0 |
Figure 3E | Two-way repeated measures ANOVA Post-hoc Šídák’s multiple comparisons test LIV-VI aIC-BLA neurons F-I curve | ANOVA Results: Treatment; p = 0.0013, F (3, 72) = 5.837 Current; p < 0.0001, F (1.959, 141.0) = 802.5 Interaction; p < 0.0001, F (24, 567) = 6.468 Multiple Comparisons: 0pA Extinction vs. Reinstatement Mean difference = 0.000; 50pA Extinction vs. Reinstatement p >0.9999, t = 0.1718, df = 243.0; 100pA Extinction vs. Reinstatement p = 0.8899, t = 1.237, df = 243.0; 150pA Extinction vs. Reinstatement p >0.9999, t = 0.2492, df = 243.0; 200pA Extinction vs. Reinstatement p = 0.5553, t = 1.723, df = 243.0; 250pA Extinction vs. Reinstatement p = 0.0341, t = 2.919, df = 243.0; 300pA Extinction vs. Reinstatement p = 0.0030, t = 3.636, df = 243.0; 350pA Extinction vs. Reinstatement p = 0.0003, q = 4.203, df = 243.0; 400pA Extinction vs. Reinstatement p < 0.0001, t = 4.578, df = 243.0. |
Figure 3F | Two-tailed Unpaired t-test LIV-VI aIC-BLA neurons Action Potential Threshold Extinction Reinstatement | t-test Results: p = 0.0076 t = 2.887 df = 27 Difference between means; 6.621 ± 2.293 R squared, 0.2359 |
Figure 3G | Two-tailed Unpaired t-test LIV-VI aIC-BLA neurons Membrane Time Constant Extinction Reinstatement | t-test Results: p = 0.0153 t = 2.589 df = 27 Difference between means ;7.931 ± 3.064 R squared; 0.1988 |
Figure 4 |
Figure 4A | Two-way repeated measures ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons F-I Curve Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p = 0.0014, F (5, 109) = 4.281 Current; p < 0.0001, F (1.990, 216.9) = 1218 Interaction; p < 0.0001, F (40, 872) = 4.978 Multiple Comparisons: 0pA Saccharin 1x vs. Quinine 1x Mean difference = 0.000 Saccharin 1x vs. Saccharin 2x Mean difference = 0.000 Saccharin 1x vs. CTA Retrieval Mean difference = 0.000 Saccharin 1x vs. Extinction Mean difference = 0.000 Saccharin 1x vs. Reinstatement Mean difference = 0.000 Quinine 1x vs. Saccharin 2x Mean difference = 0.000 Quinine 1x vs. CTA Retrieval Mean difference = 0.000 Quinine 1x vs. Extinction Mean difference = 0.000 Quinine 1x vs. Reinstatement Mean difference = 0.000 Saccharin 2x vs. CTA Retrieval Mean difference = 0.000 Saccharin 2x vs. Extinction Mean difference = 0.000 Saccharin 2x vs. Reinstatement Mean difference = 0.000 CTA Retrieval vs. Extinction Mean difference = 0.000 CTA Retrieval vs. Reinstatement Mean difference = 0.000 Extinction vs. Reinstatement Mean difference = 0.000 50pA Saccharin 1x vs. Quinine 1x p>0.9999q = 0.2195, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.9993, q = 0.5023, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.9998, q = 0.3831, df = 981.0; Saccharin 1x vs. Extinction p = 0.9986, q = 0.5691, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.9999q = 0.3539, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.9960, q = 0.7153, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.9981, q = 0.6123, df = 981.0; Quinine 1x vs. Extinction p = 0.9945, q = 0.7626, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9988, q = 0.5535, df = 981.0; Saccharin 2x vs. CTA Retrieval p>0.9999, q = 0.1553, df = 981.0; Saccharin 2x vs. Extinction p>0.9999, q = 0.1132, df = 981.0; Saccharin 2x vs. Reinstatement p>0.9999, q = 0.1112, df = 981.0; CTA Retrieval vs. Extinction p>0.9999, q = 0.2589, df = 981.0; CTA Retrieval vs. Reinstatement p>0.9999, q = 0.02435, df = 981.0; Extinction vs. Reinstatement p>0.9999, q = 0.2084, df = 981.0; 100pA Saccharin 1x vs. Quinine 1x p = 0.9991, q = 0.5199, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.8659, q = 1.608, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.9941, q = 0.7759, df = 981.0; Saccharin 1x vs. Extinction p = 0.9933, q = 0.7975, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.9924, q = 0.8188, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.6709, q = 2.107, df = 981.0; Quinine 1x vs. CTA Retrieval p >0.9999, q = 0.2082, df = 981.0; Quinine 1x vs. Extinction p >0.9999, q = 0.3161, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9431, q = 1.292, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.4875, q = 2.499, df = 981.0; Saccharin 2x vs. Extinction p = 0.6016, q = 2.257, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.9970, q = 0.6698, df = 981.0; CTA Retrieval vs. Extinction p >0.9999, q = 0.1487, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.8745, q = 1.579, df = 981.0; Extinction vs. Reinstatement p = 0.8967, q = 1.500, df = 981.0 150pA Saccharin 1x vs. Quinine 1x p = 0.9491, q = 1.258, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.8741, q = 1.258, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.3926, q = 2.71, df = 981.0; Saccharin 1x vs. Extinction p>0.9999, q = 0.2459, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.9985, q = 0.5798, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.5798, q = 2.818, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.937, q = 1.324, df = 981.0; Quinine 1x vs. Extinction p = 0.9882, q = 0.9008, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9983, q = 0.5934, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.0233, q = 4.404, df = 981.0; Saccharin 2x vs. Extinction p = 0.8426, q = 0.8426, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.6995, q = 0.6995, df = 981.0; CTA Retrieval vs. Extinction p = 0.6431, q = 2.168, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.7735, q = 1.868, df = 981.0; Extinction vs. Reinstatement p>0.9999, q = 0.3023, df = 981.0 200pA Saccharin 1x vs. Quinine 1x p = 0.4777, q = 2.521, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.9511, q = 1.246, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.0346, q = 4.219, df = 981.0; Saccharin 1x vs. Extinction p>0.9999, q = 0.07085, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.627, q = 2.202, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.0862, q = 3.75, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.907, q = 1.46, df = 981.0; Quinine 1x vs. Extinction p = 0.5516, q = 2.363, df = 981.0; Quinine 1x vs. Reinstatement p>0.9999, q = 0.1599, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.0013, q = 5.554, df = 981.0; Saccharin 2x vs. Extinction p = 0.9756, q = 1.06, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.1668, q = 3.356, df = 981.0; CTA Retrieval vs. Extinction p = 0.0712, q = 3.854, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.8889, q = 1.529, df = 981.0; Extinction vs. Reinstatement p = 0.6782, q = 2.091, df = 981.0 250pA Saccharin 1x vs. Quinine 1x p = 0.1585, q = 3.389, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.9907, q = 0.8555, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.0038, q = 5.16, df = 981.0; Saccharin 1x vs. Extinction p >0.9999, q = 0.3001, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.1227, q = 3.547, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.0336, q = 4.233, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.9074, q = 1.459, df = 981.0; Quinine 1x vs. Extinction p = 0.1609, q = 3.379, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9998, q = 0.3641, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.0003, q = 6.078, df = 981.0; Saccharin 2x vs. Extinction p = 0.9994, q = 0.4763, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.0268, q = 4.339, df = 981.0; CTA Retrieval vs. Extinction p = 0.0066, q = 4.94, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.9838, q = 0.9659, df = 981.0; Extinction vs. Reinstatement p = 0.1238, q = 3.541, df = 981.0 300pA Saccharin 1x vs. Quinine 1x p = 0.0468, q = 4.071, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.9991, q = 0.5264, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.0006, q = 5.795, df = 981.0; Saccharin 1x vs. Extinction p = 0.9998, q = 0.3808, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.023, q = 4.41, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.0153, q = 4.591, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.9311, q = 1.354, df = 981.0; Quinine 1x vs. Extinction p = 0.046, q = 4.08, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9985, q = 0.585, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.0001, q = 6.36, df = 981.0; Saccharin 2x vs. Extinction p >0.9999, q = 0.09689, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.0073, q = 4.898, df = 981.0; CTA Retrieval vs. Extinction p = 0.0011, q = 5.594, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.9978, q = 0.6315, df = 981.0; Extinction vs. Reinstatement p = 0.0229, q = 4.411, df = 981.0 350pA Saccharin 1x vs. Quinine 1x p = 0.0058, q = 4.992, df = 981.0; Saccharin 1x vs. Saccharin 2x p = 0.9998, q = 0.4001, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.0001, q = 6.284, df = 981.0; Saccharin 1x vs. Extinction p >0.9999, q = 0.2699, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.0028, q = 5.272, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.0021, q = 5.387, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.9909, q = 0.8504, df = 981.0; Quinine 1x vs. Extinction p = 0.0092, q = 4.807, df = 981.0; Quinine 1x vs. Reinstatement p = 0.9985, q = 0.5836, df = 981.0; Saccharin 2x vs. CTA Retrieval p < 0.0001, q = 6.713, df = 981.0; Saccharin 2x vs. Extinction p >0.9999, q = 0.09312, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.001, q = 5.642, df = 981.0; CTA Retrieval vs. Extinction p = 0.0005, q = 5.914, df = 981.0; CTA Retrieval vs. Reinstatement p >0.9999, q = 0.1648, df = 981.0; Extinction vs. Reinstatement p = 0.0044, q = 5.099, df = 981.0 400pA Saccharin 1x vs. Quinine 1x p = 0.0062, q = 4.963, df = 981.0; Saccharin 1x vs. Saccharin 2x p >0.9999, q = 0.24, df = 981.0; Saccharin 1x vs. CTA Retrieval p = 0.0004, q = 5.925, df = 981.0; Saccharin 1x vs. Extinction p = 0.9991, q = 0.5192, df = 981.0; Saccharin 1x vs. Reinstatement p = 0.0014, q = 5.513, df = 981.0; Quinine 1x vs. Saccharin 2x p = 0.0034, q = 5.2, df = 981.0; Quinine 1x vs. CTA Retrieval p = 0.9991, q = 0.5284, df = 981.0; Quinine 1x vs. Extinction p = 0.0053, q = 5.027, df = 981.0; Quinine 1x vs. Reinstatement p = 0.991, q = 0.8492, df = 981.0; Saccharin 2x vs. CTA Retrieval p = 0.0002, q = 6.183, df = 981.0; Saccharin 2x vs. Extinction p >0.9999, q = 0.3014, df = 981.0; Saccharin 2x vs. Reinstatement p = 0.0008, q = 5.736, df = 981.0; CTA Retrieval vs. Extinction p = 0.0005, q = 5.857, df = 981.0; CTA Retrieval vs. Reinstatement p = 0.9997, q = 0.4195, df = 981.0; Extinction vs. Reinstatement p = 0.0013, q = 5.554, df = 981.0 |
Figure 4B | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons fAHP Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p < 0.0001, F (5, 109) = 10.64; R squared, 0.3283; Multiple Comparisons: Saccharin 1x vs. Quinine 1x p < 0.0001, q = 9.380, df = 109; Saccharin 1x vs. Saccharin 2x p = 0.7249, q = 1.985, df = 109; Saccharin 1x vs. CTA Retrieval p = 0.0127, q = 4.774, df = 109; Saccharin 1x vs. Extinction p = 0.9204, q = 1.399, df = 109; Saccharin 1x vs. Reinstatement p = 0.5239, q = 2.428, df = 109; Quinine 1x vs. Saccharin 2x p < 0.0001, q = 7.421, df = 109; Quinine 1x vs. CTA Retrieval p = 0.0035, q = 5.331, df = 109; Quinine 1x vs. Extinction p < 0.0001, q = 7.147, df = 109; Quinine 1x vs. Reinstatement p = 0.0003, q = 6.299, df = 109; Saccharin 2x vs. CTA Retrieval p = 0.4251, q = 2.647, df = 109; Saccharin 2x vs. Extinction p = 0.9997, q = 0.4017, df = 109; Saccharin 2x vs. Reinstatement p = 0.9983, q = 0.5902, df = 109; CTA Retrieval vs. Extinction p = 0.3621, q = 2.796, df = 109; CTA Retrieval vs. Reinstatement p = 0.7995, q = 1.799, df = 109; Extinction vs. Reinstatement p = 0.9868, q = 0.9191, df = 109. |
Figure 4C | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons Input Resistance Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p = 0.0213, F (5, 109) = 2.775; R squared, 0.1129; Multiple Comparisons: Saccharin 1x vs. Quinine 1x p >0.9999, q = 0.03668, df = 109; Saccharin 1x vs. Saccharin 2x p = 0.2331, q = 3.152, df = 109; Saccharin 1x vs. CTA Retrieval p = 0.9876, q = 0.9065, df = 109; Saccharin 1x vs. Extinction p = 0.9997, q = 0.4256, df = 109; Saccharin 1x vs. Reinstatement p = 0.3953, q = 2.716, df = 109; Quinine 1x vs. Saccharin 2x p = 0.2582, q = 3.075, df = 109; Quinine 1x vs. CTA Retrieval p = 0.9859, q = 0.9323, df = 109; Quinine 1x vs. Extinction p = 0.9998, q = 0.3877, df = 109; Quinine 1x vs. Reinstatement p = 0.4229, q = 2.652, df = 109; Saccharin 2x vs. CTA Retrieval p = 0.0352, q = 4.286, df = 109; Saccharin 2x vs. Extinction p = 0.5204, q = 2.435, df = 109; Saccharin 2x vs. Reinstatement p >0.9999, q = 0.2024, df = 109; CTA Retrieval vs. Extinction p = 0.9475, q = 1.262, df = 109; CTA Retrieval vs. Reinstatement p = 0.1001, q = 3.711, df = 109; Extinction vs. Reinstatement p = 0.6757, q = 2.098, df = 109. |
Figure 4D | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons Sag ratio Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p = 0.0286, F (5, 109) = 2.610; R squared, 0.1069; Multiple Comparisons: Saccharin 1x vs. Quinine 1x p = 0.9862, q = 0.9280, df = 109; Saccharin 1x vs. Saccharin 2x p = 0.5707, q = 2.327, df = 109; Saccharin 1x vs. CTA Retrieval p = 0.6972, q = 2.049, df = 109; Saccharin 1x vs. Extinction p = 0.9794, q = 1.015, df = 109; Saccharin 1x vs. Reinstatement p = 0.9643, q = 1.152, df = 109; Quinine 1x vs. Saccharin 2x p = 0.2112, q = 3.225, df = 109; Quinine 1x vs. CTA Retrieval p = 0.9784, q = 1.026, df = 109; Quinine 1x vs. Extinction p>0.9999, q = 0.1598, df = 109; Quinine 1x vs. Reinstatement p = 0.7184, q = 2.000, df = 109; Saccharin 2x vs. CTA Retrieval p = 0.0209, q = 4.543, df = 109; Saccharin 2x vs. Extinction p = 0.2415, q = 3.126, df = 109; Saccharin 2x vs. Reinstatement p = 0.9805q = 1.002, df = 109; CTA Retrieval vs. Extinction p = 0.9944, q = 0.7617, df = 109; CTA Retrieval vs. Reinstatement p = 0.2504, q = 3.099, df = 109; Extinction vs. Reinstatement p = 0.7140, q = 2.010, df = 109. |
Figure 4E | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons Action Potential Amplitude Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p = 0.0054, F (5, 109) = 3.526; R squared, 0.1392; Multiple Comparisons: Saccharin 1x vs. Quinine 1x p = 0.2342, q = 3.149, df = 109; Saccharin 1x vs. Saccharin 2x p = 0.7922, q = 1.818, df = 109; Saccharin 1x vs. CTA Retrieval p = 0.3531, q = 2.818, df = 109; Saccharin 1x vs. Extinction p = 0.8190, q = 1.746, df = 109; Saccharin 1x vs. Reinstatement p = 0.9979, q = 0.6222df = 109; Quinine 1x vs. Saccharin 2x p = 0.0087, q = 4.944, df = 109; Quinine 1x vs. CTA Retrieval p = 0.9983, q = 0.5921, df = 109; Quinine 1x vs. Extinction p = 0.9662, q = 1.137, df = 109; Quinine 1x vs. Reinstatement p = 0.5806, q = 2.305, df = 109; Saccharin 2x vs. CTA Retrieval p = 0.0129, q = 4.768, df = 109; Saccharin 2x vs. Extinction p = 0.1650, q = 3.396, df = 109; Saccharin 2x vs. Reinstatement p = 0.5804, q = 2.306, df = 109; CTA Retrieval vs. Extinction p = 0.9968, q = 0.6777, df = 109; CTA Retrieval vs. Reinstatement p = 0.7511, q = 1.922, df = 109; Extinction vs. Reinstatement p = 0.9746, q = 1.065, df = 109. |
Figure 4F | One-way ANOVA Kruskal-Wallis test Post-hoc Dunn's multiple comparisons test LIV-VI aIC-BLA neurons Action Potential Half-width Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Kruskal-Wallis test; p = 0.0002; Kruskal-Wallis statistic,24.03 Multiple Comparisons: Saccharin 1x vs. Quinine 1x p >0.9999, z = 0.6106 Saccharin 1x vs. Saccharin 2x p >0.9999, z = 0.2586; Saccharin 1x vs. CTA Retrieval p >0.9999, z = 0.04096; Saccharin 1x vs. Extinction p = 0.0485, z = 2.944; Saccharin 1x vs. Reinstatement p = 0.0200, z = 3.208; Quinine 1x vs. Saccharin 2x p >0.9999, q = 0.8658; Quinine 1x vs. CTA Retrieval p >0.9999, z = 0.6129; Quinine 1x vs. Extinction p = 0.2759, z = 2.358; Quinine 1x vs. Reinstatement p = 0.1372, z = 2.607; Saccharin 2x vs. CTA Retrieval p >0.9999, z = 0.3181; Saccharin 2x vs. Extinction p = 0.0222, z = 3.179; Saccharin 2x vs. Reinstatement p = 0.0085, z = 3.448; CTA Retrieval vs. Extinction p = 0.0312, z = 3.079; CTA Retrieval vs. Reinstatement p = 0.0115, z = 3.366; Extinction vs. Reinstatement p >0.9999, z = 0.1880. |
Figure 4G | One-way ANOVA Post-hoc Tukey’s multiple comparisons LIV-VI aIC-BLA neurons Membrane Time Constant Saccharin 1x Quinine 1x Saccharin 2x CTA retrieval Extinction Reinstatement | ANOVA results: Treatment; p = 0.0047, F (5, 109) = 0.1419; R squared, 0.1419; Multiple Comparisons: Saccharin 1x vs. Quinine 1x p = 0.0987, q = 3.720, df = 109; Saccharin 1x vs. Saccharin 2x p = 0.4932, q = 2.495, df = 109; Saccharin 1x vs. CTA Retrieval p = 0.1046, q = 3.685, df = 109; Saccharin 1x vs. Extinction p = 0.9230, q = 1.388, df = 109; Saccharin 1x vs. Reinstatement p = 0.0022, q = 5.525, df = 109; Quinine 1x vs. Saccharin 2x p = , 0.9484 q = 1.257, df = 109; Quinine 1x vs. CTA Retrieval p = 0.9999, q = 0.3489, df = 109; Quinine 1x vs. Extinction p = 0.7139, q = 2.010, df = 109; Quinine 1x vs. Reinstatement p = 0.7124, q = 2.014, df = 109; Saccharin 2x vs. CTA Retrieval p = 0.9798, q = 1.011, df = 109; Saccharin 2x vs. Extinction p = 0.9894, q = 0.8761, df = 109; Saccharin 2x vs. Reinstatement p = 0.2138, q = 3.216, df = 109; CTA Retrieval vs. Extinction p = 0.7866, q = 1.833, df = 109; CTA Retrieval vs. Reinstatement p = 0.4978, q = 2.484, df = 109; Extinction vs. Reinstatement p = 0.0896, q = 3.777, df = 109. |