Article Figures & Data
Figures
- Figure 1.
Experimental setup and task design. A, Schematic of the experimental setting. The recording sessions take place inside the testing area (shown in yellow) next to the group housing. Four antennas (shown in green) are installed at the ceiling of the testing area. The experimenter has access to the XBI (shown in orange) from the back. The data acquisition system is shown in blue. B, Animal K working on the XBI inside the testing area. The wireless head stages are protected with a cap (shown in purple). C, Data flow during recordings. Neural signals are collected, digitized, and sent wirelessly to the receiving antennas. At the same time the animal interacts through a touch screen with the XBI. Neural signals are sent to the acquisition system together with time stamps for trial starts for offline synchronization with behavioral data. D, Behavioral task design. The animals are required to perform two consecutive reaches to a first target in the lower row and a second target in the upper row. Upon touching the starting position, two possible targets appear in both rows together with two color cues next to the starting position, indicating the correct target in each row. Circles with white dashed lines show additional possible reach target (not visible to the animal). Reach target and colors are randomized trial-wise. The second reach is greyed out as analysis in this study only includes times until touching the first target.
- Figure 2.
Wireless recording in home enclosure. Each row represents the neural signal from an example electrode. One electrode per recorded area is shown. Red traces show the raw data trace from one example trial. The second column shows the extracted average wave forms after preprocessing and spike sorting, based on a principal component (PC) analysis. Corresponding clusters in first two PCs are shown on the right. For visualization purposes we only show the first two PCs. For spike sorting we used additional features including third PC, nonlinear energy, and interspike interval. Colored traces below raw data represent spike trains for each sorted unit in the example trial. Shaded areas for wave forms indicate standard deviation.
- Figure 3.
Neural selectivity for spatial task parameters. Each subfigure shows the target position dependent tuning for one example unit from each recorded area. For each unit, a raster plot and averaged spike densities are shown aligned to three different task events (instruction of reach target, go cue, movement onset). Colors correspond to four different horizontally aligned target positions (shown at the top). Shaded areas show standard error of the mean. The panel on the right shows the mean firing rate of the unit from the highlighted alignment for the four-target positions. Error bars correspond to 95% CI.
In this issue
