DNA Recognition by Cys2His2 Zinc Finger Proteins

SA Wolfe, L Nekludova, CO Pabo - Annual review of biophysics …, 2000 - annualreviews.org
… have shown that serine at position 3 seems to prefer thymine as the central base (18, 19,
110), but in the QGSR Zif variant, the serine at position 3 specifies cytosine (EI Ramm, SA Wolfe

The protective role of sparse vegetation in wind erosion

SA Wolfe, WG Nickling - Progress in physical geography, 1993 - journals.sagepub.com
Vegetation, in the form of cover crops, wind strips or postharvest residues is typically used
as a means of managing agricultural soils prone to wind erosion. Similarly, trees and shrubs …

[HTML][HTML] The NIH somatic cell genome editing program

…, M Vandsburger, JK Watts, KD Wells, SA Wolfe… - Nature, 2021 - nature.com
The move from reading to writing the human genome offers new opportunities to improve
human health. The United States National Institutes of Health (NIH) Somatic Cell Genome …

A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity

…, E Ma, S Mane, GJ Hannon, ND Lawson, SA Wolfe… - Science, 2010 - science.org
Dicer is a central enzyme in microRNA (miRNA) processing. We identified a Dicer-independent
miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In …

Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases

…, MB Noyes, LJ Zhu, ND Lawson, SA Wolfe - Nature …, 2008 - nature.com
Direct genomic manipulation at a specific locus is still not feasible in most vertebrate model
organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced …

[HTML][HTML] Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish

…, L Ebarasi, C Betsholtz, S Schulte-Merker, SA Wolfe… - Developmental cell, 2015 - cell.com
The widespread availability of programmable site-specific nucleases now enables targeted
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

…, Z Weng, Y Dong, V Koteliansky, SA Wolfe… - Nature …, 2016 - nature.com
The combination of Cas9, guide RNA and repair template DNA can induce precise gene
editing and the correction of genetic diseases in adult mammals. However, clinical …

[HTML][HTML] Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites

…, A Wakabayashi, GD Stormo, MH Brodsky, SA Wolfe - Cell, 2008 - cell.com
We describe the comprehensive characterization of homeodomain DNA-binding specificities
from a metazoan genome. The analysis of all 84 independent homeodomains from D. …

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

…, RL Bogorad, T Zatsepin, V Koteliansky, SA Wolfe… - Nature …, 2017 - nature.com
Efficient genome editing with Cas9–sgRNA in vivo has required the use of viral delivery
systems, which have limitations for clinical applications. Translational efforts to develop other …

Multicolor CRISPR labeling of chromosomal loci in human cells

…, P Reyes-Gutierrez, SA Wolfe… - Proceedings of the …, 2015 - National Acad Sciences
The intranuclear location of genomic loci and the dynamics of these loci are important
parameters for understanding the spatial and temporal regulation of gene expression. Recently it …