DNA Recognition by Cys2His2 Zinc Finger Proteins
SA Wolfe, L Nekludova, CO Pabo - Annual review of biophysics …, 2000 - annualreviews.org
… have shown that serine at position 3 seems to prefer thymine as the central base (18, 19,
110), but in the QGSR Zif variant, the serine at position 3 specifies cytosine (EI Ramm, SA Wolfe…
110), but in the QGSR Zif variant, the serine at position 3 specifies cytosine (EI Ramm, SA Wolfe…
The protective role of sparse vegetation in wind erosion
SA Wolfe, WG Nickling - Progress in physical geography, 1993 - journals.sagepub.com
Vegetation, in the form of cover crops, wind strips or postharvest residues is typically used
as a means of managing agricultural soils prone to wind erosion. Similarly, trees and shrubs …
as a means of managing agricultural soils prone to wind erosion. Similarly, trees and shrubs …
[HTML][HTML] The NIH somatic cell genome editing program
The move from reading to writing the human genome offers new opportunities to improve
human health. The United States National Institutes of Health (NIH) Somatic Cell Genome …
human health. The United States National Institutes of Health (NIH) Somatic Cell Genome …
A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity
Dicer is a central enzyme in microRNA (miRNA) processing. We identified a Dicer-independent
miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In …
miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In …
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
Direct genomic manipulation at a specific locus is still not feasible in most vertebrate model
organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced …
organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced …
[HTML][HTML] Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish
…, L Ebarasi, C Betsholtz, S Schulte-Merker, SA Wolfe… - Developmental cell, 2015 - cell.com
The widespread availability of programmable site-specific nucleases now enables targeted
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …
gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate …
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
The combination of Cas9, guide RNA and repair template DNA can induce precise gene
editing and the correction of genetic diseases in adult mammals. However, clinical …
editing and the correction of genetic diseases in adult mammals. However, clinical …
[HTML][HTML] Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites
…, A Wakabayashi, GD Stormo, MH Brodsky, SA Wolfe - Cell, 2008 - cell.com
We describe the comprehensive characterization of homeodomain DNA-binding specificities
from a metazoan genome. The analysis of all 84 independent homeodomains from D. …
from a metazoan genome. The analysis of all 84 independent homeodomains from D. …
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
…, RL Bogorad, T Zatsepin, V Koteliansky, SA Wolfe… - Nature …, 2017 - nature.com
Efficient genome editing with Cas9–sgRNA in vivo has required the use of viral delivery
systems, which have limitations for clinical applications. Translational efforts to develop other …
systems, which have limitations for clinical applications. Translational efforts to develop other …
Multicolor CRISPR labeling of chromosomal loci in human cells
…, P Reyes-Gutierrez, SA Wolfe… - Proceedings of the …, 2015 - National Acad Sciences
The intranuclear location of genomic loci and the dynamics of these loci are important
parameters for understanding the spatial and temporal regulation of gene expression. Recently it …
parameters for understanding the spatial and temporal regulation of gene expression. Recently it …