User profiles for G. Hilgen

Dr Gerrit Hilgen

Associate Professor, Health & Life Sciences, Northumbria University, UK
Verified email at northumbria.ac.uk
Cited by 1130

[HTML][HTML] Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa

…, R Bronstein, S Mehrotra, M Farkas, G Hilgen… - Nature …, 2018 - nature.com
… Schematic representation of alternative splice variants of the E1A reporter (e) and denaturing
PAGE and autoradiography using a phosphoimager (f), n = 3; g Northern blot analysis …

Human-induced pluripotent stem cells generate light responsive retinal organoids with variable and nutrient-dependent efficiency

D Hallam, G Hilgen, B Dorgau, L Zhu, M Yu, S Bojic… - Stem Cells, 2018 - academic.oup.com
… (E–G): Higher magnifications of recover in expressing photoreceptors and their morphology
(Recov, red) including putative outer segments and axon terminals. (H): Photoreceptor outer …

[HTML][HTML] Next generation organoid engineering to replace animals in cancer drug testing

…, X Todd, S Todryk, RG Gieling, G Hilgen… - Biochemical …, 2023 - Elsevier
… Dormancy, or G 0 cell cycle arrest, and its contribution to treatment resistance is also well
known. In the dormant state, also known as quiescence, cancer cells accumulate additional …

[PDF][PDF] Unsupervised spike sorting for large-scale, high-density multielectrode arrays

G Hilgen, M Sorbaro, S Pirmoradian, JO Muthmann… - Cell reports, 2017 - cell.com
We present a method for automated spike sorting for recordings with high-density, large-scale
multielectrode arrays. Exploiting the dense sampling of single neurons by multiple …

Systematic comparison of retinal organoid differentiation from human pluripotent stem cells reveals stage specific, cell line, and methodological differences

…, J Collin, R Queen, G Hilgen… - Stem cells …, 2019 - academic.oup.com
A<sc>bstract</sc> A major goal in the stem cell field is to generate tissues that can be utilized
as a universal tool for in vitro models of development and disease, drug development, or …

[HTML][HTML] Decellularised extracellular matrix-derived peptides from neural retina and retinal pigment epithelium enhance the expression of synaptic markers and light …

B Dorgau, M Felemban, G Hilgen, M Kiening, D Zerti… - Biomaterials, 2019 - Elsevier
… The DMMB reagent was prepared as follows: 16 mg DMMB in 1 L double-distilled water
containing 3.04 g glycine, 1.6 g NaCl and 95 ml of 0.1 M Acetic Acid. The pH of the solution was …

Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line-and method-dependent

V Chichagova, G Hilgen, A Ghareeb, M Georgiou… - Stem Cells, 2020 - academic.oup.com
Induced pluripotent stem cell (iPSC)-derived retinal organoids provide a platform to study
human retinogenesis, disease modeling, and compound screening. Although retinal organoids …

Transplanted pluripotent stem cell-derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration

D Zerti, G Hilgen, B Dorgau, J Collin, M Ader… - Stem Cells, 2021 - academic.oup.com
Retinal dystrophies often lead to blindness. Developing therapeutic interventions to restore
vision is therefore of paramount importance. Here we demonstrate the ability of pluripotent …

Incorporating microglia‐like cells in human induced pluripotent stem cell‐derived retinal organoids

…, G Hilgen, J Collin, R Queen, G Chung… - Journal of cellular …, 2023 - Wiley Online Library
Microglia are the primary resident immune cells in the retina. They regulate neuronal survival
and synaptic pruning making them essential for normal development. Following injury, they …

Human retinal organoids provide a suitable tool for toxicological investigations: a comprehensive validation using drugs and compounds affecting the retina

…, J Collin, R Queen, G Hilgen… - Stem Cells …, 2022 - academic.oup.com
… • Intraperitoneal: 4 k/kg followed by 2 doses of 2 g/kg after 24 h and 48 h respectively … •
Intraperitoneal: 4 k/kg followed by 2 doses of 2 g/kg after 24 h and 48 h respectively …