Abstract
Fyn, protein tyrosine kinase, and its substrates were highly concentrated in the postsynaptic density (PSD) fraction prepared from the rat forebrain. There were a number of Fyn substrates unique to the PSD fraction. One of the major substrates in the PSD fraction was found to be a concanavalin A-binding glycoprotein, PSD-gp180, which is the N-methyl-D-aspartate (NMDA) receptor subunit epsilon 2 (NR2B). Western blotting and immunoprecipitation supported the phosphorylation of epsilon 2 by Fyn. NMDA receptor subunit epsilon 1 (NR2A) was also a substrate for Fyn. These results suggest that Fyn is involved in the modulation of synaptic efficacy through the phosphorylation of synapse-specific substrates such as the NMDA receptor/channel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Cell Fractionation
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Concanavalin A
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Macromolecular Substances
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Phosphorylation
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Phosphotyrosine / analysis
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Prosencephalon / metabolism*
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Protein-Tyrosine Kinases / metabolism*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-fyn
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Rats
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Rats, Wistar
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Receptors, N-Methyl-D-Aspartate / chemistry
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Receptors, N-Methyl-D-Aspartate / isolation & purification
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Receptors, N-Methyl-D-Aspartate / metabolism*
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Subcellular Fractions / enzymology
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Substrate Specificity
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Synapses / metabolism*
Substances
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Macromolecular Substances
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Proto-Oncogene Proteins
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Receptors, N-Methyl-D-Aspartate
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Concanavalin A
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Phosphotyrosine
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Protein-Tyrosine Kinases
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Fyn protein, rat
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Proto-Oncogene Proteins c-fyn