Abstract
The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CA1 Region, Hippocampal / metabolism*
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Calcium / metabolism
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Diagnostic Imaging / methods*
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Fluorescence Resonance Energy Transfer / methods
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Gene Expression / genetics
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Green Fluorescent Proteins / genetics
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Membrane Proteins / genetics*
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Mice
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Mice, Transgenic
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Microscopy, Fluorescence / methods
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Pyramidal Cells / metabolism*
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Response Elements / genetics*
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Tetracycline / pharmacology
Substances
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Membrane Proteins
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Green Fluorescent Proteins
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Tetracycline
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Calcium
Grants and funding
This work was supported by Precursory Research for Embryonic Science and Technology (PRESTO) of the Japan Science and Technology Agency (JST) and the Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI Grants 21800091, 24700403, 25116528 and 26115530 to M.S., MEXT/JSPS KAKENHI Grant 22110006 and a Human Frontier Science Program grant to Y.H. and the Regional Innovation Cluster Program (City Area Type, Central Saitama Area) to J.N., M.O. and K.G.-A. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.