The scaffold protein JIP3 functions as a downstream effector of the small GTPase ARF6 to regulate neurite morphogenesis of cortical neurons

Atsushi Suzuki; Chihiro Arikawa; Yuji Kuwahara; Kouichi Itoh; Masatomo Watanabe; Hiroshi Watanabe; Teruhiko Suzuki; Yuji Funakoshi; Hiroshi Hasegawa; Yasunori Kanaho

doi:10.1016/j.febslet.2010.05.020

The scaffold protein JIP3 functions as a downstream effector of the small GTPase ARF6 to regulate neurite morphogenesis of cortical neurons

FEBS Lett. 2010 Jul 2;584(13):2801-6. doi: 10.1016/j.febslet.2010.05.020. Epub 2010 May 21.

Authors

Atsushi Suzuki¹, Chihiro Arikawa, Yuji Kuwahara, Kouichi Itoh, Masatomo Watanabe, Hiroshi Watanabe, Teruhiko Suzuki, Yuji Funakoshi, Hiroshi Hasegawa, Yasunori Kanaho

Affiliation

¹ Department of Physiological Chemistry, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.

PMID: 20493856
DOI: 10.1016/j.febslet.2010.05.020

Abstract

The small GTPase ADP-ribosylation factor 6 (ARF6) plays crucial roles in a wide variety of cell functions. To better understand the molecular mechanisms of ARF6-mediated signaling and cellular functions, we sought new ARF6-binding proteins in the mouse brain. We identified the signaling scaffold protein JNK-interacting protein 3 (JIP3), which is exclusively expressed in neurons, as a downstream effector of ARF6. Overexpression of a unique dominant negative mutant of ARF6, which was unable to interact with JIP3, and knockdown of JIP3 in mouse cortical neurons stimulated the elongation and branching of neurites. These results provide evidence that ARF6/JIP3 signaling regulates neurite morphogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADP-Ribosylation Factor 6
ADP-Ribosylation Factors / genetics
ADP-Ribosylation Factors / metabolism*
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Animals
Axons / metabolism
Cells, Cultured
Cerebral Cortex / cytology
Dendrites / metabolism
Mice
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Neurites / metabolism*
Neurogenesis / genetics
Neurogenesis / physiology*
Neurons / cytology
Neurons / metabolism*
Protein Binding / genetics
Protein Binding / physiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

ADP-Ribosylation Factor 6
Adaptor Proteins, Signal Transducing
Mapk8ip3 protein, mouse
Nerve Tissue Proteins
Spag9 protein, mouse
ADP-Ribosylation Factors
ARF6 protein, human
Arf6 protein, mouse