Abstract
Our previous work has shown that PSD-95/SAP90 is required for NMDA receptor-mediated thermal hyperalgesia. To address the role of PSD-95/SAP90 in chronic pain, the present study investigated the effect of the deficiency of PSD-95/SAP90 on nerve injury-induced neuropathic pain. Following unilateral L5 spinal nerve injury, mechanical and thermal hyperalgesia developed within 3 days and persisted for 9 days or longer on the injured side. The intrathecal administration of antisense oligodeoxynucleotide specifically against PSD-95/SAP90, but not sense or missense oligodeoxynucleotide, dose-dependently delayed the onset of tactile allodynia and thermal hyperalgesia. These results suggest that PSD-95/SAP90 might be involved in the central mechanisms of the development of chronic neuropathic pain.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Behavior, Animal / drug effects
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Behavior, Animal / physiology
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Disease Models, Animal
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Disks Large Homolog 4 Protein
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Dose-Response Relationship, Drug
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Foot / innervation
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Foot / physiopathology
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Hyperalgesia / drug therapy
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Hyperalgesia / metabolism
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Hyperalgesia / physiopathology
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Intracellular Signaling Peptides and Proteins
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Male
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Membrane Proteins
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Nerve Tissue Proteins / antagonists & inhibitors
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Nerve Tissue Proteins / deficiency*
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Neuralgia / drug therapy
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Neuralgia / metabolism*
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Neuralgia / physiopathology
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Oligodeoxyribonucleotides, Antisense / pharmacology*
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Pain Measurement / drug effects
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Pain Threshold / drug effects
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Pain Threshold / physiology
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Peripheral Nerve Injuries
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Peripheral Nerves / metabolism*
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Peripheral Nerves / physiopathology
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Peripheral Nervous System Diseases / drug therapy
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Peripheral Nervous System Diseases / metabolism*
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Peripheral Nervous System Diseases / physiopathology
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Physical Stimulation
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Rats
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Rats, Sprague-Dawley
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Spinal Nerve Roots / injuries
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Spinal Nerve Roots / physiopathology
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Spinal Nerve Roots / surgery
Substances
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Disks Large Homolog 4 Protein
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Dlg4 protein, rat
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Oligodeoxyribonucleotides, Antisense
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postsynaptic density proteins