Abstract
LIM homeodomain (LIM-HD) proteins play key roles in a variety of developmental processes throughout the animal kingdom. Here we show that the LIM-binding protein Chip acts as a cofactor for the Drosophila LIM-HD family member Apterous (Ap) in wing development. We define the domains of Chip required for LIM-HD binding and for homodimerization and show that mutant proteins deleted for these domains act in a dominant-negative fashion to disrupt Ap function. Our results support a model for multimeric complexes containing Chip and Ap in transcriptional regulation. This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Genetically Modified
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Dimerization
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Drosophila / genetics
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Drosophila / growth & development
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Drosophila / physiology*
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Drosophila Proteins*
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Gene Expression Regulation, Developmental
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Genes, Reporter
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Homeodomain Proteins*
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Insect Proteins / metabolism
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LIM-Homeodomain Proteins
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Recombinant Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Wings, Animal / abnormalities
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Wings, Animal / growth & development
Substances
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Chi protein, Drosophila
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Drosophila Proteins
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Homeodomain Proteins
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Insect Proteins
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LIM-Homeodomain Proteins
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Nuclear Proteins
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Recombinant Proteins
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Transcription Factors
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ap protein, Drosophila