Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats☆
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Highlights
• CP-AMPA receptors are increased at synapses on GnRH neurons of ProE rats. • Functional blockade of CP-AMPA receptors depresses synaptic responses of ProE rats. • Functional blockade of CP-AMPA receptors attenuates the LH surge.
Abbreviations
ANOVA
analysis of variance
CNQX
6-cyano-7-nitroquinoxaline-2,3-dione
CP-AMPARs
calcium permeable AMPA-type glutamate receptors
EGTA
ethylene glycol tetraacetic acid
GFP
green fluorescent protein
GnRH
gonadotropin-releasing hormone
HEPES
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
LH
luteinizing hormone
mEPSCs
miniature excitatory postsynaptic currents
NASPM
1-naphthyl acetyl spermine
OVX
ovariectomized
PPR
paired-pulse ratio
Key words
CP-AMPA receptor
synapse
GnRH
Trafficking
estrus cycle
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- ☆
Disclosure summary: The authors have nothing to disclose.
- †
These authors contributed equally this work.
Copyright © 2013 The Authors. Published by Elsevier Ltd.