Regeneration, Repair, and Developmental NeuroscienceResearch PaperOptical analysis of developmental changes in synaptic potentiation in the neonatal rat corticostriatal projection
Highlights
▶We optically examined developmental changes in LTP in the neonatal cortico-striatum. ▶The EPSP-related slow signal could be detected from the postnatal day 1 (P1) stage. ▶From the P9 stage, the slow signal was potentiated by tetanus. ▶The LTP was maintained for a longer time in the 3W stage than in the 2W stage. ▶The results suggest dynamic change in characteristics of LTP during ontogenesis.
Section snippets
Preparations
Experiments were carried out in accordance with the guidelines of the US National Institutes of Health and Komazawa Women's University for the care and use of laboratory animals. All efforts were made to minimize the number of animals used and their suffering. All of the experiments were performed at Komazawa Women's University. Neonatal Wistar rats (Saitama Experimental Animals Supply Co., Saitama, Japan) at postnatal day 1–21 (P1–P21) were decapitated under ether anesthesia. Their brains were
Optical detection of the LTP in the corticostriatal projection
Fig. 1 illustrates examples of optical recordings made in a postnatal 17-day (P17) corticostriatal slice preparation stained with the voltage-sensitive oxonol dye NK3630 (RH482). The upper and lower panels show optical responses detected prior to and 2 h after tetanic stimulation (100 Hz/1 s duration, 1 shot), respectively. The signals were evoked by electrical stimulation to the corticostriatal projection, and the recordings were made by averaging four trials using a 464-element photodiode
Discussion
In this study, we applied optical imaging with voltage-sensitive dyes to the corticostriatal projection in neonatal rat cortical slice preparations. The results obtained are summarized as follows: (1) The LTP in the corticostriatal projection is detectable with the optical imaging technique, (2) the EPSP can be potentiated with tetanic stimulation from the P9 stage, and (3) after LTP induction, the potentiation is maintained for a longer time in the postnatal 3W stage than in the 2W stage. We
Acknowledgments
This research was supported by grants from the Monbu-Kagaku-Sho of Japan, Human Frontier Science program, and Opto-Medical Institute Inc in Takanawa, Tokyo.
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