Cellular neuroscienceDistribution and neuropeptide coexistence of nucleobindin-2 mRNA/nesfatin-like immunoreactivity in the rat CNS
Section snippets
Animals
Male Sprague–Dawley rats (6–8 weeks; Scanlab, Stockholm, Sweden) were housed under 12-h dark/light conditions (lights on at 06:00 h and off at 18:00 h) in a temperature-controlled environment with free access to standard rat chow and tap water. All experiments had been approved by the local ethical committee (Stockholms Norra Djurförsöksetiska Nämnd) and the studies were carried out in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC). All efforts were
Results
The distribution of NUCB2 mRNA was investigated by in situ hybridization (Fig. 1). The four oligonucleotide probes, complementary to non-overlapping segments of the NUCB2 cDNA, tested separately, yielded qualitatively similar in situ hybridization patterns, albeit with varying signal strength. The highest signal-to-noise ratio resulted from a combination of probes against base pairs 438–486 and 1402–1450; in the results described below and figures this combination was used. Furthermore, the
Discussion
Here we have studied the rat CNS distribution of NUCB2 mRNA and its newly described protein fragment nesfatin-1 which has been ascribed anorexigenic properties. We show a wide distribution in the hypothalamus and a restricted set of other brain regions, as well as its colocalization with several neuropeptides implicated in the control of energy metabolism. The brain distribution is summarized schematically in Fig. 10 (NUCB2/nesfatin cells indicated on plates from Paxinos et al., 2007). Our
Acknowledgments
The authors gratefully acknowledge the donors of antisera used in this study (see Experimental Procedures). We also thank Drs. Staffan Cullheim and Tomas Hökfelt and their respective laboratories for sharing expertise and equipment, and Dr. David Lyons for helpful comments on the manuscript. Emanuela Santini is gratefully acknowledged for expert assistance with Western blot experiments. Financial support for this study and publication was provided by the European Neuroscience Institutes
References (63)
- et al.
Blocking melanin-concentrating hormone MCH(1) receptor affects rat sleep-wake architecture
Eur J Pharmacol
(2008) Interactions between the “cognitive” and “metabolic” brain in the control of food intake
Physiol Behav
(2007)- et al.
The raphe nuclei of the cat brain stem: a topographical atlas of their efferent projections as revealed by autoradiography
Brain Res
(1976) Hypothalamic cocaine- and amphetamine-regulated transcript (CART) neurons: histochemical relationship to thyrotropin-releasing hormone, melanin-concentrating hormone, orexin/hypocretin and neuropeptide Y
Brain Res
(1999)- et al.
Cocaine- and amphetamine-regulated transcript-immunoreactivity in the rat sympatho-adrenal axis
Neurosci Lett
(2000) - et al.
Leptin activates hypothalamic CART neurons projecting to the spinal cord
Neuron
(1998) - et al.
Coexistence of dopamine and neurotensin in hypothalamic arcuate and periventricular neurons
Brain Res
(1983) - et al.
Extracellular TNFR1 release requires the calcium-dependent formation of a nucleobindin 2-ARTS-1 complex
J Biol Chem
(2006) Single unit activity of locus coeruleus neurons in behaving animals
Prog Neurobiol
(1986)Raphe pallidus and raphe obscurus projections to the intermediolateral cell column in the rat
Brain Res
(1981)
ACTH-(1–24) and alpha-MSH antagonize feeding behavior stimulated by kappa opiate agonists
Peptides
Hypothalamic regulation of sleep
Neuropsychopharmacology
The need to feed: homeostatic and hedonic control of eating
Neuron
Direct hypothalamo-autonomic connections
Brain Res
A direct projection from the ventromedial nucleus and retrochiasmatic area of the hypothalamus to the medulla and spinal cord of the rat
Neurosci Lett
Neural mechanisms for the functional coupling of autonomic, endocrine and somatomotor responses in adaptive behavior
Brain Res
The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm
J Biol Chem
Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper proteinMolecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein
Biol Chem Hoppe Seyler
Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain
Endocrinology
Cocaine- and amphetamine-regulated transcript (CART) and food intake: Behavior in search of anatomy
Drug Dev Res
Brain regulation of food intake and appetite: molecules and networks
J Intern Med
Hypocretin/orexin- and melanin-concentrating hormone-expressing cells form distinct populations in the rodent lateral hypothalamus: relationship to the neuropeptide Y and agouti gene-related protein systems
J Comp Neurol
Neuropeptide Y innervation and neuropeptide-Y-Y1-receptor-expressing neurons in the paraventricular hypothalamic nucleus of the mouse
Neuroendocrinology
Melanocortin-4 receptor is required for acute homeostatic responses to increased dietary fat
Nat Neurosci
Norepinephrine-containing neurons: changes in spontaneous discharge patterns during sleeping and waking
Science
Anatomy and regulation of the central melanocortin system
Nat Neurosci
Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridization histochemistry
Histochemistry
Characterization of CART neurons in the rat and human hypothalamus
J Comp Neurol
Role of melanocortinergic neurons in feeding and the agouti obesity syndrome
Nature
Dominant and recessive inheritance of morbid obesity associated with melanocortin 4 receptor deficiency
J Clin Invest
Distribution and coexpression patterns of nucleobindin-2 (NUCB2) mRNA and nesfatin-1 peptide in the rat hypothalamus
Soc Neurosci Abstr
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