Behavioural neuroscienceNicotinamide treatment induces behavioral recovery when administered up to 4 hours following cortical contusion injury in the rat
Section snippets
Subjects
Thirty-four Sprague–Dawley rats (Harlan, Indianapolis, IN, USA), 3–4 months of age were included in this experiment. All experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee and the study was conducted in a facility certified by the American Association for the Accreditation of Laboratory Animal Care on the ethical use of animals and every attempt was made to minimize the number of animals used and their suffering. Rats were maintained on a
NAM analysis
There were significant serum NAM concentration differences between groups as analyzed by a one-way ANOVA [F(4,27)=34.52, P<0.05]. Post hoc analyses revealed that all NAM treatment groups had significantly higher serum concentrations than saline or sham groups (P<0.05) (see Fig. 1).
Bilateral tactile adhesive removal task
The latency to remove both stimuli from each trial was averaged together for analysis using a 5×7 ANOVA with repeated measures. Group (15-min NAM, 4-h NAM, 8-h NAM, CCI-saline, sham) and day (2, 4, 6, 8, 10, 14, and
Discussion
This study demonstrated that administration of a six-dose regimen of NAM following frontal CCI had a dose-dependent effect on the level of circulating serum NAM. Administration of NAM starting at any of the initial time points post-CCI (15-min, 4-h, or 8-h) significantly elevated serum NAM compared with the animals not receiving NAM administration. Interestingly, the 15-min injection resulted in significantly higher levels of serum NAM than either of the two administration time points.
The main
Acknowledgments
Research supported by NINDS (NS045647-03) to M.R.H.
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