Functional organization of preoptic vasotocin and isotocin neurons in the brain of rainbow trout: central and neurohypophysial projections of single neurons
Section snippets
Animals
Immature rainbow trout (Oncorhynchus mykiss; fork length 17–19 cm, body weight 50–70 g) were obtained from a commercial source (Sapporo Nijimasu Co. Ltd., Sapporo, Japan), and were maintained in a 0.5 ton circular tank (100 cm in diameter and 80 cm in depth) at 15 °C under 12-h light/dark photoperiod for more than a week. Animals were obtained in July, October, and December. Four fish were used for the double color immunofluorescence and two fish for the Golgi-like immunohistochemistry. All
Organization of the preoptic vt and it neurons
A confocal image of the hemisected brain viewed from the ventricular side illustrates an overall sagittal aspect of preoptic NSCs that expand two-dimensionally beneath the ependymal layer (Fig. 1A). NSCs form a paired nucleus located very close to the third ventricle. NSCs that occupy the dorsocaudal portion of the preoptic area comprise the magnocellular preoptic nucleus (PM). The ventrolateral portion of the mass of NSCs comprises the anterior parvocellular part of the preoptic region (PPa).
Discussion
In the present study, we clarified the neuroanatomical organization of the preoptic neurosecretory system in a teleost model using a combination of immunohistochemical and intracellular staining methods. Confocal double immunofluorescence characterized the cytoarchitecture of the PM, that is, (1) clustering of the same types of NSCs, (2) medial localization of VT clusters and lateral localization of IT clusters, and (3) contact among VT neurons at the proximal processes. Golgi-like
Acknowledgements
We thank E. Ito and H. Ando for helpful advice. This work was supported by grants-in-aid for Scientific Research and for JSPS Fellows of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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