Neuron
Volume 87, Issue 3, 5 August 2015, Pages 563-575
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Article
Synaptic Vesicle Exocytosis at the Dendritic Lobules of an Inhibitory Interneuron in the Mammalian Retina

https://doi.org/10.1016/j.neuron.2015.07.016Get rights and content
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Highlights

  • Dendritic exocytosis is mediated by L-type Ca2+ channels

  • Two readily releasable pools of vesicles with distinct release kinetics

  • Graded light-evoked depolarization triggers graded exocytosis without synaptic ribbons

  • Tighter Ca2+ channel coupling to exocytosis during postnatal development

Summary

Ribbon synapses convey sustained and phasic excitatory drive within retinal microcircuits. However, the properties of retinal inhibitory synapses are less well known. AII-amacrine cells are interneurons in the retina that exhibit large glycinergic synapses at their dendritic lobular appendages. Using membrane capacitance measurements, we observe robust exocytosis elicited by the opening of L-type Ca2+ channels located on the lobular appendages. Two pools of synaptic vesicles were detected: a small, rapidly releasable pool and a larger and more slowly releasable pool. Depending on the stimulus, either paired-pulse depression or facilitation could be elicited. During early postnatal maturation, the coupling of the exocytosis Ca2+-sensor to Ca2+ channel becomes tighter. Light-evoked depolarizations of the AII-amacrine cell elicited exocytosis that was graded to light intensity. Our results suggest that AII-amacrine cell synapses are capable of providing both phasic and sustained inhibitory input to their postsynaptic partners without the benefit of synaptic ribbons.

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