Review
Current concept in neural regeneration research: NSCs isolation, characterization and transplantation in various neurodegenerative diseases and stroke: A review

https://doi.org/10.1016/j.jare.2013.04.005Get rights and content
Under a Creative Commons license
open access

Abstract

Since last few years, an impressive amount of data has been generated regarding the basic in vitro and in vivo biology of neural stem cells (NSCs) and there is much far hope for the success in cell replacement therapies for several human neurodegenerative diseases and stroke. The discovery of adult neurogenesis (the endogenous production of new neurons) in the mammalian brain more than 40 years ago has resulted in a wealth of knowledge about stem cells biology in neuroscience research. Various studies have done in search of a suitable source for NSCs which could be used in animal models to understand the basic and transplantation biology before treating to human. The difficulties in isolating pure population of NSCs limit the study of neural stem behavior and factors that regulate them. Several studies on human fetal brain and spinal cord derived NSCs in animal models have shown some interesting results for cell replacement therapies in many neurodegenerative diseases and stroke models. Also the methods and conditions used for in vitro culture of these cells provide an important base for their applicability and specificity in a definite target of the disease. Various important developments and modifications have been made in stem cells research which is needed to be more specified and enrolment in clinical studies using advanced approaches. This review explains about the current perspectives and suitable sources for NSCs isolation, characterization, in vitro proliferation and their use in cell replacement therapies for the treatment of various neurodegenerative diseases and strokes.

Keywords

Neural stem cells
Characterization
Neurodegenerative diseases
Stroke
Regeneration

Cited by (0)

Dr. Sandeep Kumar Vishwakarma, Ph.D (Genetics,) from Osmania University, Hyderabad, Andhra Pradesh, India specializes in Stem Cell biology and Molecular Genetics. He has got excellence in gene cloning, expression, protein purification, cell culture, Neo-organogenesis, Bioinformatics and a variety of basic and advanced tools and techniques applied in biological research. With more than five years of research experience, he has spear headed neural stem cell work and published one book chapter and more than 30 articles in various reputed National and International Journals. Presently he is involved in bridging the gap between basic sciences to application of neural stem cell research and investigating new therapeutic targets in a variety of neurodegerative diseases. He has got a number of honor’s and best paper awards at both National and International levels. He is the member of several esteemed societies including “The Cytometry Society” and is involved in a variety of high quality research projects as leading investigator.

Dr. Avinash Bardia, Ph.D (Genetics) from Osmania University, Hyderabad, experiences in Molecular Biology and Microbial Genetics. He has got brilliancy in genetic analysis of microbes and animals and a variety of basic and advanced applied tools and techniques in biological research. With more than five years of research experience in basic and clinical sciences, he has published more than 20 articles in various reputed National and International Journals. He has been a recipient of several awards and honors both at National and International levels. He is the member of several esteemed societies including “The Cytometry Society” and is involved in a variety of leading research projects.

Dr. Santosh Tiwari, a doctorate in Genetics from Osmania University, Hyderabad specializes in the area of Microbial Genetics and Immunology. After spending a brief time at Cancer Genetics & Research Facility in University of Florida, USA, he made his way back to India. His passion towards writing paved way to one of the world’s leading pharmaceutical company. With more than 10 years research and a over 3 years experience as a professional scientific writer, he has more than 60 International publications to his credit. He has been a recipient of several awards and laurels both at National and International levels. He is the member of several esteemed societies including the Society for Translational and Regenerative Medicine. He has certifications from NIH, USA and Adis Wolters Kluwer, New Zealand in Clinical Research and Scientific Writing.

Dr. Syed Ameer Basha Paspala, a neurosurgeon and leading scientist in the area of neural stem cell biology has done his doctorate from JNTU, Hyderabad, Andhra Pradesh, India. He is involved in developing new therapeutic targets and stem cell therapy approach for the treatment of neurodegenerative diseases. He has published more than 20 articles in various national and international reputed journals. Presently, he is involved in bridging gap between basic and clinical research in neural stem cell biology and regeneration of brain. He has got a number of honor’s and best paper awards at both National and International levels. He is a great asset to the scientific society and man of tribute to the research perception.

Dr. Aleem Ahmed Khan, a leading scientist in hepatic stem cell biology has done his doctorate from Osmania University, Hyderabad, Andhra Pradesh, India in the area of Cell Biology and Transplantation Immunology. He has pioneered the hepatic stem cell technology for the treatment of liver cirrhosis. Recently he investigated trans-differentiation of hepatic stem cells into insulin producing pancreatic β-cells without any genetic manipulation. He has published more than 100 articles in various national and international reputed journals and has supervised more than 15 Ph.D scholars. Presently, he is involved in area of stem cell biology and neural regeneration research.

Peer review under responsibility of Cairo University.

  1. Download : Download full-size image

Copyright © 2013 Production and hosting by Elsevier B.V.