Original Contributionα-Tocopherol suppresses lipid peroxidation and behavioral and cognitive impairments in the Ts65Dn mouse model of Down syndrome
Section snippets
Animals
All mice were a result of crossing B6EiC3Sn a/A-Ts(17<16>)65Dn (Ts65Dn) females (originally obtained from The Jackson Laboratory, Bar Harbor, ME, USA) to C57BL/6JEi × C3H/HeSnJ (B6EiC3Sn) F1 males (The Jackson Laboratory). Control mice (2N, diploid) for this experiment were normosomic littermates of the Ts65Dn mice with the same genetic background (B6EiC3Sn). Animals from the same litter were group-housed and maintained on a 12-h/12-h light/dark schedule. All mice were chromosomally genotyped.
α-Tocopherol-supplemented diet improves the performance of Ts65Dn mice on the elevated-plus maze
The results of the elevated-plus maze test indicated significant differences between control-diet-fed 2N mice and Ts65Dn mice (Fig. 1). The preference for open versus closed arms is expressed as an open arm ratio, calculated as the percentage of time spent in the open arms (open arm/(open + closed arm)). The open arm ratio for control-diet-fed Ts65Dn mice (41.7%) was approximately eight times higher than that of the control-diet-fed 2N mice (5.2%). α-Tocopherol supplementation reduced the open
Discussion
This study attempted to characterize the behavioral effects induced by chronic supplementation with α-tocopherol from the embryonic stage in Ts65Dn mice. To the best of our knowledge, no previous study has used a mouse model to examine the effects of α-tocopherol from the embryonic stage. Ts65Dn mice fed a control diet exhibited the following behavioral features in comparison to control-diet 2N mice: (a) less anxiety and regardlessness in the elevated-plus maze task, (b) impaired spatial
Acknowledgments
This study was partially supported by Grant-in-Aid for Young Scientists (A) 22680051 and Scientific Research (B) 22300242 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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