Lineage-restricted neural precursors survive, migrate, and differentiate following transplantation into the injured adult spinal cord
Introduction
Numerous studies over the last two decades have shown that grafting of embryonic day-14 fetal spinal cord tissue (E14/FSC) into the injured spinal cord rescues axotomized neurons from retrograde cell death and atrophy (Bregman and Reier, 1986, Mori et al., 1997), reduces scar formation (Houle, 1992), and promotes host axonal regeneration (Diener and Bregman, 1998b, Tessler et al., 1988). In the adult, host axons penetrate the graft, but do not transverse transplants to reenter host neuropil. Graft-derived fibers also project into host tissue (Jakeman and Reier, 1991, Reier et al., 1986). Although FSC grafts have been successful in improving functional recovery following complete spinal transection of neonates (Diener and Bregman, 1998a, Howland et al., 1995, Miya et al., 1997), little recovery occurs in adult models (Bregman et al., 1993, Stokes and Reier, 1992). Despite potential benefits, the use of fetal transplants has serious shortcoming including issues of supply, storage, cellular heterogeneity, and quality control.
E14/FSC transplants consist primarily of lineage-restricted neural precursors, both neuronal (NRP)- and glial(GRP)-restricted precursors, as well as small populations of multipotent neural stem cells (NSCs) and differentiated cells (Kalyani and Rao, 1998). Previous studies have shown that NSCs grafted into non-neurogenic regions of intact and injured adult CNS survive poorly (Lepore et al., 2004) and differentiate mostly into glial cells (Cao et al., 2001). In contrast, lineage-restricted precursors survive for long periods of time and differentiate into neurons and glia, respectively (Han et al., 2002, Han et al., 2004, Lepore et al., 2004). However, previous studies have raised concerns about the ability of grafted NSCs and NRPs to survive in the toxic environment of the injury site and to differentiate into mature neurons without the necessary instructive environment (Cao et al., 2002). We therefore reasoned that comparing the fate of E14/FSC transplants and transplants of a defined population of lineage-restricted precursors (NRPs and GRPs) in the injured adult spinal cord, using cells derived from alkaline phosphatase (AP) transgenic rats, will allow for elucidating the sequence of events that follows grafting of fetal cells in the injured adult CNS, including survival, migration, and differentiation.
Using a lateral funiculus injury model, we found an early and extensive cell loss following acute transplants of E14/FSC. Surviving precursors expanded to fill the entire lesion by 3 weeks post-transplantation. E14/FSC grafts integrated and extended long processes into host spinal cord, differentiated into neurons, astrocytes, and oligodendrocytes and showed variability in migration out of the transplant site. Grafts of defined NRP/GRP cells filled the cavity without significant early cell loss, differentiated into mature CNS phenotypes and showed consistent cellular migration out of the injury site, even when grafted into the acute injury. This work suggests that mixed lineage-restricted precursor grafts generate a microenvironment that protects the cells from the detrimental effects of the injured CNS and provides them with a permissive niche for survival, differentiation, and migration. Furthermore, NRP/GRP grafts represent a practical alternative to fetal tissue transplants because of the ability to isolate, expand, store, and genetically manipulate these cells.
Section snippets
Cell isolation and culture
NRPs and GRPs were isolated from embryonic day-13.5 transgenic Fischer 344 rats that express the marker gene, human placental alkaline phosphatase (AP). This transgenic animal has previously been characterized (Kisseberth et al., 1999, Mujtaba et al., 2002). Briefly, embryos were isolated in DMEM/F12 (Invitrogen; Carlsbad, CA). Trunk segments were incubated in collagenase Type I (10 mg/mL; Worthington Biochemicals; Lakewood, NJ)/dispase II (20 ng/mL; Roche Diagnostics; Indianapolis, IN)/HBSS
E14 spinal cord grafts
The phenotypic composition of E14/FSC was assessed to determine the specific cell types that make up the graft. DAPI staining shows the cellular pattern of E14/FSC (Fig. 1A). A large proportion of the cells expressed nestin (Fig. 1B), an early neural marker expressed by both NSCs and lineage-restricted precursors. Cells of the E14 FSC also expressed either E-NCAM (Fig. 1C) or A2B5 (Fig. 1D), markers of NRPs and GRPs, respectively. Only small numbers of cells expressed markers of NSCs (Sox-2) or
Discussion
Fetal spinal cord tissue has been used as a transplant source for two decades, but the events that follow grafting into the injured spinal cord were not well understood because of difficulties in cell detection and the limited knowledge of neural precursor biology at the time. Our studies were designed to elucidate the sequence of events that follow FSC transplantation into the injured spinal cord and at the same time address important issues concerning the fate of neural precursor cells in the
Acknowledgments
We acknowledge all the members of our group for their assistance, particularly Carla Tyler-Poltz for excellent tissue culture, Dr. Birgit Neuhuber and Dr. Steve Han for critical discussions, Dr. Timothy Himes and Theresa Connors for their technical expertise, and Andrea Ketschek for help in early experiments. A.C.L. thanks the Neuroscience graduate program for support and Stephen, Leopold, and Molly for a much needed diversion. I.F. is supported by NIH grants NS24707 and NS 37515. This work has
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2022, International Review of NeurobiologyCitation Excerpt :Although transplantation of pluripotent NEPs into the normal or lesioned adult spinal cord limits differentiation of NEPs to a glial lineage (Cao et al., 2001), transplanting neuronal-restricted precursors into the normal (Han et al., 2002) and contused adult spinal cord yields some mature neuronal phenotypes but also many undifferentiated cells, two to eight weeks post-transplantation (Cao et al., 2001). Subsequent studies of transplantation of mixed NRP and GRP cell cultures demonstrated the necessity of including GRPs to ensure neuronal survival and differentiation post-transplantation (Lepore & Fischer, 2005). One of the most successful experiments demonstrating the potential of transplantation of a mixed population of NRPs and GRPs to treat the injured spinal cord was demonstrated in a dorsal column hemisection model (Bonner et al., 2011).
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