Archival ReportNovelty-Seeking Behaviors and the Escalation of Alcohol Drinking After Abstinence in Mice Are Controlled by Metabotropic Glutamate Receptor 5 on Neurons Expressing Dopamine D1 Receptors
Section snippets
Animals
The mGluR5KD-D1 strain has been described previously (24). Animals were 8 to 16 weeks of age at the beginning of experimental procedures and were housed in Plexiglas home cages (30 × 40 × 20 cm) with two to six animals per cage in a room with controlled temperature (22°C ± 1°C) and humidity (55% to 65%). Mice had access to standard lab chow (Labofeed H, WPiK, Kcynia, Poland) and water ad libitum. Experiments were conducted in accordance with the European Union guidelines regarding the care and
Generation and Characterization of mGluR5KD-D1 Mice
Mice with selective knockdown of mGluR5 messenger RNA in D1-expressing neurons (mGluR5KD-D1 mice) were bred as congenic with the C57BL/6N strain (24). To confirm the cell-type specificity of transgene expression, we used immunohistochemical staining of the green fluorescent protein, which is expressed by the transgene. The strongest signal was observed in the striatum, but green fluorescent protein-expressing cells were also detected in the lower layers of the cortex, the basolateral amygdala,
Discussion
The loss of mGluR5 from neurons expressing dopamine D1 receptors abolishes sensation-seeking behavior, which extends the previous observation from mGluR5 KO mice (21). We found that all novelty-seeking behaviors were attenuated in mGluR5KD-D1 mice, which also displayed mildly decreased locomotor activity across all tests performed, except for the water maze. In contrast, mice with complete mGluR5 KO have been reported to exhibit enhanced activity in a novel environment (22), which could be due
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Authors JRP and MS contributed equally to this work.