Cellular colocalization of dopamine D1 mRNA and D2 receptor in rat brain using a D2 dopamine receptor specific polyclonal antibody

doi:10.1016/S0278-5846(00)00125-1

Progress in Neuro-Psychopharmacology and Biological Psychiatry

Volume 24, Issue 7, October 2000, Pages 1127-1149

https://doi.org/10.1016/S0278-5846(00)00125-1 Get rights and content

Abstract

1.
1. The main objective of this work was to investigate the extent of cellular colocalization of dopamine D1 and D2 receptors in the rat brain. A double labeling technique, that combined immunocytochemical labeling of the D2 receptor using polyclonal antibodies raised against the third intracellular loop of the short isoform of the human D2 receptor in combination with in situ hybridization detecting D1 mRNA expression, was designed to accomplish this goal.
2.
2. The specificity of the antisera obtained was confirmed by immunoprecipitation assay, Western blot analysis, and immunocytochemistry on D2R transfected cells and murine brain tissue. Western blot using the D2 receptor antibody revealed a specific broad band centered at 67 kDa in transfected cells and a major protein of 88 kDa corresponding to D2R expressed in the caudate-putamen, to a lesser extent in the cortex, and not at all detected in the hypothalamic region.
3.
3. The content of neurons double-labeled for $D1 D2$ receptors was observed at in differing intensities in the dorsal endopiroform nucleus, the intercalated nucleus of amygdala, the anterior part of the cortical nucleus amygdala, the nucleus of the lateral olfactory tract, the piriform cortex, the parabrachial nucleus, the supraoptic nucleus and the parabigeminal nucleus. All other regions of the brain revealed neurons expressing either D1 or D2 dopamine receptors but not both at that same time.
4.
4. These results clearly demonstrated that specific neurons expressed both receptors D1 and D2, and that this colocalization was restricted to particular regions of the rat brain.

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Full length original papers Experimental studyCellular colocalization of dopamine D1 mRNA and D2 receptor in rat brain using a D2 dopamine receptor specific polyclonal antibody

Abstract

Brain Res.

J. Biol. Chem.

Brain Res.

Brain Res.

Biochem. Biophy. Res. Commun.

J. Biol. Chem.

Biochem. Biophy. Res. Com.

Neurosci.

Trends Neurosci.

Anal. Biochem.

Brain Res.

Neurosci.

Bioch. Biophy. Res. Commun.

Life Sci.

Brain Res.

Neurosci.

Anal. Biochem.

Trends in Pharmacol. Sci.

Eur. J. Pharmacol.

Trends Neurosci.

Biochem. Biophysic. Res. Communic.

Brain Res.

Eur. J. Pharmacol.

A novel radioiodinated high affinity ligand for the D2-dopamine receptor-Characterization of its binding in bovine anterior pituary membranes

FEBS Lett.

Molecular characterization of dopamine receptors

Am. J. Hyperten.

Buffer systems and transfer parameters for semi-dry electroblotting with a horizontal apparatus

Development of polyclonal anti-D2 dopamine receptor antibodies to fusion proteins: Inhibition of D2 receptor-G protein interaction

J. Neurochem.

Intracellular studies on the dopamine-induced firing inhibition of neostriatal neurons in vitro: evidence for D1 receptor involvement

Neurosci.

Antibodies raised against purified beta-adrenergic receptors specifically bind beta-adrenergic ligands

Antisera specific for D2 dopamine receptors

Biochem. J.

Full length original papers Experimental study
Cellular colocalization of dopamine D1 mRNA and D2 receptor in rat brain using a D2 dopamine receptor specific polyclonal antibody