ArticlesSocial Stress Effects on Territorial Marking and Ultrasonic Vocalizations in Mice
Section snippets
General methods
Research was conducted in compliance with the Animal Care Welfare Act, and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles in the Guide for Care and Use of Laboratory Animals, National Research Council, National Academic Press, Washington, DC, 1996.
Procedure
Because there is individual variability in territorial urine marking, subjects were prescreened for baseline urine marking. Ten of 15 mice displayed high marking levels during a baseline test, and were pair matched with another male with similar marking levels. The other five mice were excluded from the experiment. One member of each of the five subject pairs received SD. The other members of each pair did not receive SD, and served as control mice (NOSD). Twenty-four hours following SD or
Procedure
Mice received 8 days of social experience and were prescreened for ultrasonic courtship vocalizations. As in Experiment 1, mice (n = 22) were pair matched using their baseline territorial urine marking as matching criterion. Eleven mice received repeated SD, and 11 mice were not exposed to SD. SD mice received 3 successive days of SD, and were tested for ultrasonic courtship vocalizations to female cage bedding 30-min post-SD on each of the 3 days. In addition, mice were tested for ultrasonic
General discussion
There is a striking relationship between social dominance and the occurrence of male-typical androgen-dependent behaviors in house mice. For example, dominant males are more aggressive than subordinates, establish and defend territories (13), and sire more offspring (15). We currently report a prolonged decrease in territorial urine marking and a transient decrease in ultrasonic courtship vocalizations following SD. Mice that received acute SD displayed significantly less territorial urine
Acknowledgements
Lucille A. Lumley, Ph.D., was supported as a National Research Council Fellow. The authors thank George A. Saviolakis, M.D., Ph.D., for editorial comments, and Denise Morton and Lily Lin for their excellent assistance in quantifying urine markings and/or ultrasonic vocalizations. Intramural research was funded by U.S. Army Medical Research and Material Command. The views of the authors do not purport to reflect the position of the Dept. of the Army or the Dept. of Defense (paragraph 4-3), AR
References (62)
- et al.
Hormone-dependent aggression in male rats is proportional to serum testosterone concentration but sexual behavior is not
Physiol. Behav.
(1990) - et al.
A quantitative study of serum testosterone, sex accessory organ weight growth and the development of intermale aggression in the mouse
Horm. Behav.
(1977) - et al.
Evidence for episodic secretion of testosterone in laboratory mice
Steroids
(1975) - et al.
Effects of medial preoptic lesions on male mouse ultrasonic vocalizations and copulatory behavior
Brain Res. Bull.
(1981) - et al.
Androgen levels and components of aggressive behavior in men
Horm. Behav.
(1987) - et al.
Role of anxiety in subordinate male mice sexual behavior
Pharmacol. Biochem. Behav.
(1992) - et al.
Hormonal reactions to fighting in rat coloniesProlactin rises during defence, not during offence
Physiol. Behav.
(1992) - et al.
Neuroendocrine response patterns in dominant and subordinant mice
Horm. Behav.
(1978) - et al.
Learning of submissive behavior in miceA new model
Behav. Process.
(1982) - et al.
Behavioral, hormonal and neurochemical characteristics of aggressive α-mice
Brain Res.
(1989)
Acute and repeated exposure to social conflict in male golden hamstersIncreases in plasma POMC-peptides and cortisol and decreases in plasma testosterone
Horm. Behav.
Intracerebroventricular administration of corticotropin-releasing factor induced c-fos mRNA expression in brain regions related to stress responsesComparison with pattern of c-fos mRNA induction after stress
Brain Res.
Regulation of urine marking in male and female miceEffects of sex steroids
Horm. Behav.
The temporal dynamics of the stress response
Neurosci. Biobehav. Rev.
Pontine control of the urinary bladder and external urethral sphincter in the rat
Brain Res.
The relation between social rank and plasma corticosterone in mice
Gen. Comp. Endocrinol.
Age-associated changes in plasma testosterone levels in male mice and their relationship to social dominance or subordinance
Horm. Behav.
Effects of chronic treatment with testosterone priopionate on aggression and hormonal levels in intact male mice
Psychoneuroendocrinology
Urinary marking and tendency to investigate novelty in Mus musculus.
Behav. Neural Biol.
Short- and long-term effects of aggressive behavior on urinary marking in Mus musculus.
Behav. Neural Biol.
Behavioral sensitization to cocaine after a brief social stress is accompanied by changes in Fos expression in the murine brainstem
Brain Res.
The effect of testosterone, estradiol and dihydrotestosterone on male mouse (Mus musculus) ultrasonic vocalizations
Horm. Behav.
Courtship vocalizations in male Swiss–Webster miceEffects of hormones and sexual experience
Physiol. Behav.
Social status and ultrasonic vocalizations of male mice
Behav. Biol.
Intracranial androgenic and estrogenic stimulation of male-typical behaviors in house mice (Mus domesticus)
Horm. Behav.
Classical conditioning of stress-induced analgesia
Physiol. Behav.
Continuous social defeat induces an increase of endogenous opioids in discrete brain areas of the Mongolian gerbil
Peptides
Manipulation of aggressive behavior in adult DBA/2/Bg and C57BL/10/Bg male mice implanted with testosterone in silastic tubing
Horm. Behav.
Serum testosterone, agonistic behavior, and dominance in inbred strains of mice
Horm. Behav.
Olfaction and brainstem circuits of reproductive behavior in the rat
Role of the flank gland in vasopressin induced scent marking behavior in the hamster
Brain Res. Bull.
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