Elsevier

Peptides

Volume 6, Issue 1, 2 January 1985, Pages 45-52
Peptides

Article
A new antiserum with conformational specificity for LHRH: Usefulness for radioimmunoassay and immunocytochemistry

https://doi.org/10.1016/0196-9781(85)90075-0Get rights and content

Abstract

We have produced and characterized a new high titer, highly specific antiserum for luteinizing hormone-releasing hormone (LHRH), and demonstrated its usefulness for radioimmunoassay (RIA) and immunocytochemistry. The antiserum can be used at a final dilution of 1:500,000 to 1:600,000 for RIAs with a sensitivity of 0.2 pg/tube. Both the amino and carboxy terminal ends of the LHRH molecule are required for antibody recognition, and the antigenic determinant appears to be part of a three-dimensional structure of LHRH. Fragments of LHRH and other brain peptides are not recognized by the antiserum. Using immunocytochemical techniques, we have localized LHRH-containing neurons in the medial basal hypothalamus of the rhesus monkey, guinea pig, and rat. Staining of LHRH fibers and cell bodies was eliminated by preabsorbtion of the immune serum with synthetic LHRH. This antiserum should be useful in studies that require quantification of very low amounts of LHRH and in studies that require correlation between immunocytochemical localization and tissue content or secretion of LHRH.

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    The work described in this article, Publication No 1378 of the Oregon Regional Primate Research Center, was supported by grants HD15888 (WEE), NS18848, HD16793 (OKR), NS18989 (MJK), HD16022 (JAR) from the National Institutes of Health.

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