Elsevier

Journal of Neuroimmunology

Volume 53, Issue 2, September 1994, Pages 133-141
Journal of Neuroimmunology

Myelin localization of a central nervous system ligand for L-selectin

https://doi.org/10.1016/0165-5728(94)90023-XGet rights and content

Abstract

L-selectin is a lectin-like receptor involved in lymphocyte attachment to lymph node high endothelial venules (HEV). Previously, we showed that L-selectin also participates in the in vitro attachment of lymphocytes to central nervous system (CNS) white matter. Use of an L-selectin chimera demonstrated ligand sites within CNS white matter but not the peripheral nervous system (PNS). Now employing higher resolution mapping, including EM cytochemistry, we localize the ligands to the actual myelin sheaths of CNS neurons. In the shiverer mouse, which lacks compact myelin, ligands are greatly diminished. Comparison of the myelin-associated ligand with the previously characterized HEV-ligands demonstrates a number of differences.

References (66)

  • A. Roach et al.

    Characterization of cloned cDNA representing rat meylin basic protein: absence of expression in brain of shiverer mutant mice

    Cell

    (1983)
  • K. Selmaj et al.

    Non-specific oligodendrocyte cytotoxicity mediated by soluble products of activated T cell lines

    J. Neuroimmunol.

    (1991)
  • L.M. Stoolman

    Adhesion molecules controlling lymphocyte migration

    Cell

    (1989)
  • Y. Suzuki et al.

    Sulfated glycolipids are ligands for a lymphocyte homing receptor, L-selectin (LECAM-1), binding epitope in sulfated sugar chain

    Biochem. Biophys. Res. Commun.

    (1993)
  • H. Wekerle et al.

    Cellular immune reactivity within the CNS

    Trends Neurosci.

    (1986)
  • D. Baker et al.

    Mechanisms of immune-mediated demyelinating disease of the central nervous system

    Neurochem. Res.

    (1991)
  • J.L. Baron et al.

    Surface expression of alpha 4 integrin by CD4 T cells is required for their entry into brain parenchyma

  • S. Baumhueter et al.

    Binding of L-Selectin to the vascular sialomucin, CD34

    Science

    (1993)
  • E.L. Berg et al.

    The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor

    J. Cell Biol.

    (1991)
  • M. Bevilacqua et al.

    Selectins: A family of adhesion receptors

    Cell

    (1991)
  • A.H. Cross et al.

    Homing to central nervous system vasculature by antigen-specific lymphocytes. I. Localization of 14C-labeled cells during acute, chronic, and relapsing experimental allergic encephalomyelitis

    Lab. Invest.

    (1990)
  • J. Dawson et al.

    The monoclonal antibody MEL-14 can block lymphocyte migration into a site of chronic inflammation

    Eur. J. Immunol.

    (1992)
  • W.M. Gallatin et al.

    A cell surface molecule involved in organ-specific homing of lymphocytes

    Nature

    (1983)
  • J.S. Geoffroy et al.

    Demonstration that a lectin-like receptor (gp90MEL) directly mediates adhesion of lymphocytes to high endothelial venules of lymph nodes

    J. Cell Biol.

    (1989)
  • D.M. Hammond et al.

    Double-negative T cells from MRL-lpr/lpr mice mediate cytolytic activity when triggered through adhesion molecules and constitutively express perform gene

    J. Exp. Med.

    (1993)
  • A. Hänninen et al.

    Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid binding to islet endothelium

    J. Clin. Invest.

    (1993)
  • M.E. Hemler

    VLA proteins in the integrin family: Structures, functions, and their role on leukocytes

    Annu. Rev. Immunol.

    (1990)
  • W.F. Hickey et al.

    T-lymphocyte entry into the central nervous system

    J. Neurosci. Res.

    (1991)
  • K. Huang et al.

    A lymphocyte homing receptor (L-selectin) mediates the in vitro attachment of lymphocytes to myelinated tracts of the central nervous system

    J. Clin. Invest.

    (1991)
  • Y. Imai et al.

    Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor

    J. Cell Biol.

    (1991)
  • Y. Imai et al.

    Further characterization of the interaction between L-selectin and its endothelial ligands

    Glycobiology

    (1992)
  • M.A. Jutila et al.

    Function and comparison of the neutrophil MEL-14 antigen in vivo: comparison with LFA-1 and MAC-1

    J. Immunol.

    (1989)
  • K. Kawai et al.

    Enhancing effects of irrelevant lymphocytes on adoptive transfered experimentally allergic encephalomyelitis

    J. Neuroimmunol.

    (1993)
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