Elsevier

Journal of Neuroscience Methods

Volume 45, Issues 1–2, October–November 1992, Pages 127-134
Journal of Neuroscience Methods

Research paper
Chronic drug infusion into the scala tympani of the guinea pig cochlea

https://doi.org/10.1016/0165-0270(92)90050-NGet rights and content

Abstract

This research describes a unique, effective and inexpensive delivery system to provide discrete quantities of drugs on a chronic basis to the inner ear. The amount of the drug administered and specific timing of each administration are under investigator control. A micro-injection system mounted atop an animal's head is shown to permit repeated application of agents which effectively block neural responsiveness (tetrodotoxin) on a daily basis for periods up to 2 weeks. Cannulation of the inner ear and chronic delivery of control substances (artificial perilymph) do not affect function. This system may be used to administer drugs to other compartments of the body (e.g., the brain) on a chronic basis for neurophysiologic and neuropharmacologic investigations.

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Cited by (34)

  • Round window membrane intracochlear drug delivery enhanced by induced advection

    2014, Journal of Controlled Release
    Citation Excerpt :

    However, drug delivery to the inner ear is challenging due to its small size and anatomical location in the temporal bone. Intra-cochlear delivery of drugs or genes has been successfully accomplished in animal models by injection through the round window membrane [4], injection into the endolymphatic space via scala media [5,6] and endolymphatic sac [7], and injection or infusion into the perilymphatic space via the semicircular canals [8], scala vestibuli [9,10], and most commonly the scala tympani [11–13]. Drug delivery is generally accomplished via a syringe pump [14] or an osmotic pump [15] for continuous infusion, or a reciprocating pump for zero net volume delivery [16].

  • Ultrasound-aided microbubbles facilitate the delivery of drugs to the inner ear via the round window membrane

    2013, Journal of Controlled Release
    Citation Excerpt :

    Methods for local delivery can include intratympanic or intracochlear approaches. Intracochlear drug delivery is achieved via a cochleostomy through the round window membrane (RWM) or directly through the otic capsule, and a variety of devices, including syringe [1], osmotic pumps [4], microinjector [5], and a reciprocating drug delivery system [6], have been employed. Although intracochlear application can achieve greater bioavailability of drugs entering the inner ear than the intratympanic approach, the former usually requires invasive surgical manipulation to interrupt the inner ear structure, and may increase the risk of deafness [3].

  • Murine intracochlear drug delivery: Reducing concentration gradients within the cochlea

    2010, Hearing Research
    Citation Excerpt :

    Insertion depths of 102, 127, 153, and 178 μm were created on micro drills of 175 μm and 100 μm diameters. The use of silicone insertion stops on the infusion tubing has been reported in the literature to limit the depth of penetration (Kingma et al., 1992; Chen et al., 2006; Johnson et al., 2007). For the present study, more effective seals were produced by direct bonding to the polyimide cannulae without insertion stops, so the stops were not used.

  • Inner ear drug delivery for auditory applications

    2008, Advanced Drug Delivery Reviews
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