Elsevier

Brain Research

Volume 329, Issues 1–2, 11 March 1985, Pages 368-373
Brain Research

Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals

https://doi.org/10.1016/0006-8993(85)90553-0Get rights and content

Abstract

The distribution of specific [3H]hemicholinium-3 ([3H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[3H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [3H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [3H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.

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    This work was supported in part by grants from the Department of Defense (DAMD 17-83C-3023), the American Heart Association Arizona Affiliate (962365), United States Public Health Service Awards (MH 27257 and MH 30636), and Program Project Grant HL 20984. T.W.V. is funded by a National Research Service Award from the National Heart, Lung, and Blood Institute. W.R.R. and H.I.Y. are recipients of a Research Career Development Award (HL-00776) and a Research Scientist Development Award (MH-00095, Type II), respectively.

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