Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals
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Choline on the Move. Perspectives on the Molecular Physiology and Pharmacology of the Presynaptic Choline Transporter.
2016, Advances in PharmacologyCitation Excerpt :Prior studies by Simpson and Smart of radiolabeled choline binding to hippocampal synaptosomes had revealed Na+-dependent-binding sites that were sensitive to low concentrations of HC-3 (Simpson & Smart, 1982). Rainbow and Yamamura's groups first capitalized on the availability of [3H]HC-3 to identify anatomically defined binding sites in brain preparations (Rainbow et al., 1984; Vickroy, Fibiger, Roeske, & Yamamura, 1984; Vickroy, Roeske, Gehlert, Wamsley, & Yamamura, 1985; Vickroy, Roeske, & Yamamura, 1984). In the Coyle studies (see also Manaker, Wieczorek, & Rainbow, 1986; Vickroy, Roeske, et al., 1984), HC-3 binding to rat forebrain synaptic membranes was found to be saturable, reversible, pH dependent, and of high affinity, with a Kd of 35 nM and a Bmax of 56 fmol/mg.
Comparative effects of aluminum and ouabain on synaptosomal choline uptake, acetylcholine release and (Na<sup>+</sup>/K<sup>+</sup>)ATPase
2007, ToxicologyCitation Excerpt :Several lines of evidence support the idea that the uptake of choline appears to represent a rate-limiting step of acetylcholine synthesis (i.e., Haga, 1971; Yamamura and Snyder, 1972; Guyenet et al., 1973; Simon et al., 1976; Roskoski, 1978; Weiler et al., 1981; Meyer et al., 1982). The high-affinity choline transport by cholinergic presynaptic nerve terminals is hemicholinium-3-sensitive (Ki ≈ 10−9 to 10−7 M) and driven by the transmembrane Na+ gradient (Kuhar and Zarbin, 1978; Vickroy et al., 1985; Chatterjee et al., 1987; Saltarelli et al., 1987; Happe and Murrin, 1993; Okuda et al., 2000; Misawa et al., 2001), which is maintained by the (Na+/K+)ATPase (Stein, 1990; Nelson and Lill, 1994). During the last 20 years, a number of reports appeared to emphasize that in vivo and in vitro exposure of mammalian preparations to aluminum inhibits this P-type ATPase, a ubiquitous integral membrane pump of the plasma membrane (Lai et al., 1980; King et al., 1983; Rao, 1990, 1992; Caspers et al., 1993, 1994; Lal et al., 1993; Sarin et al., 1997; Silva and Gonçalves, 2003; Silva et al., 2005).
Light-microscopic distribution and parasagittal organisation of muscarinic receptors in rabbit cerebellar cortex
1995, Journal of Chemical NeuroanatomyComparative laminar distribution of various autoradiographic cholinergic markers in adult rat main olfactory bulb
1995, Journal of Chemical Neuroanatomy
This work was supported in part by grants from the Department of Defense (DAMD 17-83C-3023), the American Heart Association Arizona Affiliate (962365), United States Public Health Service Awards (MH 27257 and MH 30636), and Program Project Grant HL 20984. T.W.V. is funded by a National Research Service Award from the National Heart, Lung, and Blood Institute. W.R.R. and H.I.Y. are recipients of a Research Career Development Award (HL-00776) and a Research Scientist Development Award (MH-00095, Type II), respectively.