Elsevier

Brain Research

Volume 99, Issue 1, 28 November 1975, Pages 85-95
Brain Research

The effects of GABA, picrotoxin and bicuculline on the spontaneous bioelectric activity of cultured cerebellar purkinje cells

https://doi.org/10.1016/0006-8993(75)90610-1Get rights and content

Summary

Addition of GABA to the bathing medium in concentrations of 10−6 to 10−5M abolished the spontaneous bioelectric activity of rat Purkinje cells in vitro. Lower concentrations of GABA (≤ 10−6M) influenced the firing pattern of spontaneous discharges in such a way that the relative number of long interspike intervals increased. The inhibitory effect of GABA was reversible; the cells resumed normal activity after being transferred to a normal balanced salt solution. The action of GABA was antagonized by bicuculline and picrotoxin, whereas strychnine was unable to overcome the inhibition induced by GABA. Both bicuculline (10−10 to 10−6M) and picrotoxin (10−7 to 10−4M) exerted an excitatory effect on cultured Purkinje cells when added to the medium. This excitatory action suggests the existence of inhibitory synapses in cultures of rat cerebellum in which endogenous GABA is used as a transmitter. The ratio between the dose of picrotoxin and that of bicuculline necessary to produce maximal excitation was in the order of 103. Both substances showed an inhibitory action at high concentrations.

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