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Protocadherin 11 X Regulates Differentiation and Proliferation of Neural Stem Cell In Vitro and In Vivo

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Abstract

Protocadherin 11 X-linked (Pcdh11x) protein is a member of the cadherin superfamily with established roles in cell adhesion. Previous studies have shown the molecular biology and possible relevance of Pcdh11x with neurological disease in humans. However, little is known about the neurophysiological function of Pcdh11x in neural development. Here, we verified that Pcdh11x is primarily expressed in various brain areas including the cortex, hippocampus, and ventricular/subventricular zone (VZ/SVZ) at different embryonic stages. Furthermore, both in vitro and in vivo experiments showed that Pcdh11x decreased neural differentiation but increased the neural proliferation. These observations demonstrate a crucial function for Pcdh11x during the development of central nervous system.

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Acknowledgments

This study was funded by Military Medical Project (BWS11J002, BWS12J010) and National Natural Science Foundation (no. 30973096). Thanks to Zhang Xiao Chong at Ju Lu Hospital for her help with the experiment. We thank Elsevier Language Editing (Project no.: 39443) for assisting in the preparation of this manuscript. The authors would like to thank all the anonymous reviewers for their valuable comments on how to improve the quality of this paper.

Conflict of Interest

No conflicts of interest, financial or otherwise, are declared by the authors.

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Authors and Affiliations

  1. Bayi Brain Hospital, Bayi Clinical Medical Institute, Southern Medical University, Beijing, China

    Peng Zhang & Ruxiang Xu

  2. Bayi Brain Hospital, The Military General Hospital of Beijing PLA, No. 5 Nanmen Cang, Dongcheng District, Beijing, 100700, China

    Peng Zhang, Cuiying Wu, Ning Liu, Lijun Niu, Zhongjie Yan, Yanyan Feng & Ruxiang Xu

  3. Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, China

    Zhongjie Yan

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  1. Peng Zhang
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  2. Cuiying Wu
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  3. Ning Liu
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Correspondence to Ruxiang Xu.

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Peng Zhang and Cuiying Wu contribute equally to this work.

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Zhang, P., Wu, C., Liu, N. et al. Protocadherin 11 X Regulates Differentiation and Proliferation of Neural Stem Cell In Vitro and In Vivo. J Mol Neurosci 54, 199–210 (2014). https://doi.org/10.1007/s12031-014-0275-x

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  • DOI: https://doi.org/10.1007/s12031-014-0275-x

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