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Changes in the distribution of calcium calmodulin-dependent protein kinase II at the presynaptic bouton after depolarization

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Brain Cell Biology

Abstract

Phosphorylation of synapsin I by CaMKII has been reported to mobilize synaptic vesicles from the reserve pool. In the present study, the distributions of α-CaMKII and of synapsin I were compared in synaptic boutons of unstimulated and stimulated hippocampal neurons in culture by immunogold electron microscopy. CaMKII and synapsin I are located in separate domains in presynaptic terminals of unstimulated neurons. Label for α -CaMKII typically surrounds synaptic vesicle clusters and is absent from the inside of the cluster in control synapses. In contrast, intense labeling for synapsin I is found within the vesicle clusters. Following 2 minutes of depolarization in high K+, synaptic vesicles decluster and CaMKII label disperses and mingles with vesicles and synapsin I. These results indicate that, under resting conditions, CaMKII has limited access to the synapsin I in synaptic vesicle clusters. The peripheral distribution of CaMKII around vesicle clusters suggests that CaMKII-mediated declustering progresses from the periphery towards the center, with the depth of penetration into the synaptic vesicle cluster depending on the duration of CaMKII activation. Depolarization also promotes a significant increase in CaMKII immunolabel near the presynaptic active zone. Activity-induced redistribution of CaMKII leaves it in a position to facilitate phosphorylation of additional presynaptic proteins regulating neurotransmitter release.

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Acknowledgments

We thank Virginia Crocker and Rita Azzam for EM technical support. This research was supported by the Intramural Research Program of the NIH, NINDS.

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Authors and Affiliations

  1. EM Facility, NINDS, NIH, Bethesda, MD, 20892, USA

    Jung-Hwa Tao-Cheng

  2. Laboratory of Neurobiology, NINDS, NIH, Bethesda, MD, 20892, USA

    Ayse Dosemeci, Christine A. Winters & Thomas S. Reese

  3. NIH, Bldg 49, Room 3A60, Bethesda, MD, 20892-4477, USA

    Thomas S. Reese

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  1. Jung-Hwa Tao-Cheng
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  2. Ayse Dosemeci
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  3. Christine A. Winters
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  4. Thomas S. Reese
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Correspondence to Thomas S. Reese.

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Tao-Cheng, JH., Dosemeci, A., Winters, C. et al. Changes in the distribution of calcium calmodulin-dependent protein kinase II at the presynaptic bouton after depolarization. Brain Cell Bio 35, 117–124 (2006). https://doi.org/10.1007/s11068-007-9012-5

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  • DOI: https://doi.org/10.1007/s11068-007-9012-5

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