Abstract
Primer and releaser pheromones are molecules used for communication that induce species-specific responses. In contrast to primer pheromones, it is not known whether the quicker-acting releaser pheromones can affect brain gene expression. We show here that isopentyl acetate (IPA), a releaser pheromone that communicates alarm in honey bees, not only provokes a quick defensive response but also influences behavior for a longer period of time and affects brain gene expression. Exposure to IPA affected behavioral responsiveness to subsequent exposures to IPA and induced the expression of the immediate early gene and transcription factor c-Jun in the antennal lobes. Our findings blur the long-standing distinction between primer and releaser pheromone and highlight the pervasiveness of environmental regulation of brain gene expression.
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References
Baba, K., Ikeda, M., Houtani, T., Nakagawa, H., Ueyama, T., Sato, K., Sakuma, S., Yamashita, T., Tsukahara, Y., and Sugimoto, T. 1997. Odor exposure reveals non-uniform expression profiles of c-Jun protein in rat olfactory bulb neurons. Brain. Res. 774:142–148.
Boch, R., Shearer, D. A., and Stone, B. C. 1962. Identification of iso-pentyl acetate as an active component in the sting pheromone of the honey bee. Nature 195:1018–1020.
Clayton, D. F. 2000. The genomic action potential. Neurobiol. Learn. Mem. 74:185–216.
Collins, A. M., and Rothenbuhler, W. C. 1978. Laboratory test of the response to an alarm chemical, isopentyl acetate, by Apis mellifera. Ann. Entomol. Soc. Am. 71:906–909.
Gascuel J., and Masson, C. 1987. Influence of olfactory deprivation on synapse frequency in developing antennal lobe of the honeybee Apis mellifera. Neurosci. Res. Commun. 1:173–180.
Grozinger, C. M., Sharabash, N. M., Whitfield, C. W., and Robinson, G. E. 2003. Pheromone-mediated gene expression in the honey bee brain. Proc. Natl. Acad. Sci. U. S. A. 100:14519–14525.
Li, Y. and Strausfeld, N. J. 1999. Multimodal efferent and recurrent neurons in the medial lobes of cockroach mushroom bodies. J. Comp. Neurol. 409:647–663.
Morgan, S., Huryn, V., Downes, S., and Mercer, A. 1998. The effects of queenlessness on the maturation of the honey bee olfactory system. Behav. Brain. Res. 91:115–126.
Robinson, G. E., Grozinger, C. M., and Whitfield, C. W. 2005. Sociogenomics: social life in molecular terms. Nat. Rev. Genet. 6:257–270.
Winston, M. L. 1987. The Biology of the Honey Bee. Harvard University Press, Cambridge, MA.
Acknowledgements
We thank K. Pruiett for help with field work; T. Newman and M. Sen Sarma for primer design; T. Newman for assistance with molecular biology; and members of the Robinson lab and C.M. Grozinger, for comments on the manuscript. This work was funded by grants from the Fyssen Foundation to C.A. and the NIH and USDA National Research Initiative Program to G.E.R.
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Alaux, C., Robinson, G.E. Alarm Pheromone Induces Immediate–Early Gene Expression and Slow Behavioral Response in Honey Bees. J Chem Ecol 33, 1346–1350 (2007). https://doi.org/10.1007/s10886-007-9301-6
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DOI: https://doi.org/10.1007/s10886-007-9301-6