Technical characteristics of assays for HTT detection
| Method | LDL/LLoQ | Dynamic range | Specificity | Reproducibility (intra/inter) | Reference |
|---|---|---|---|---|---|
| TR-FRET | 25 pm | n.a. | Mouse and human mutant HTT | n.a. | Weiss et al., 2009 |
| TR-FRET duplex | n.a. | n.a. | Mouse and human mutant HTTMouse and human total HTT | n.a. | Weiss et al., 2011 |
| TR-FRET | n.a. | 0-4 µg of total protein/well | Endogenous mouse HTT | n.a. | Liang et al., 2014 |
| ELISA | 27 pm (2.7 fmol/well) | 0.15 pM to 30 nM | Full length (human and mouse) | <10/<20% | Massai et al., 2013 |
| MesoScale Discovery | Low/high pM | 1.5–2 log units | Human mutant HTTHuman pan HTTHuman exon1-pan HTTMouse HTT | n.a. | Macdonald et al., 2014 |
| Single-molecule counting | Low fm | 6.5/16.5–8000 fM | Mutant HTTPhosphorylated HTT | <20% | Wild et al., 2015 Fodale et al., 2017 Cariulo et al., 2017 |
| IP-FCS | 16 fm | n.a. | Expanded recombinant HTT (at high concentration also not-expanded) | n.a. | Southwell et al., 2015 |