Table 12.

Detailed results and statistical analyses related to learning and memory

Y-maze, spontaneous alternation behavior
						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
% of choices		Data structure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Arm 1	2wANOVA	Normal	32.34 ± 0.87	34.24 ± 0.92	32.77 ± 1.17	0.844	0.436	0.187	0.035	0.852	0.054	0.412	0.664	0.113	−	−	-
Arm 2	2wANOVA	Normal	35.17 ± 1.17	32.74 ± 1.1	35.18 ± 1.36	1.548	0.223	0.314	9.976	0.003	0.873	0.119	0.888	0.067	−	−	-
Arm 3	2wANOVA	Normal	32.19 ± 1.46	32.98 ± 1.09	32.04 ± 1.02	0.285	0.753	0.093	10.366	0.002	0.885	0.520	0.598	0.131	−	−	-

Chance level comparison	Test	Data structure				All t	All p value	Power	WT t	WT p value	Power	Het t	Het p value	Power	KO t	KO p value	Power
Arm 1	1S-t test	Normal				Normal	Normal	Normal	Normal	Normal	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Arm 2	1S-t test	Normal				1.465	0.148	NA	1.578	0.132	NA	-0.534	0.600	NA	1.354	0.193	NA
Arm 3	1S-t test	Normal				-1.338	0.186	NA	-0.772	0.450	NA	-0.312	0.759	NA	-1.262	0.223	NA

						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
	Test	Data structure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vs Het	WT vs KO	Het vs KO
Total number of choices	2wANOVA	Normal	43.42 ± 3.25	40.26 ± 2.54	38.47 ± 2.75	0.612	0.546	0.147	0.164	0.687	0.068	2.244	0.116	0.437	−	−	-
Number of correct choice	2wANOVA	Normal	57.46 ± 1.59	60.68 ± 1.93	57.52 ± 1.61	1.227	0.302	0.256	2.987	0.090	0.396	0.927	0.402	0.202	−	−	-
Number of type 1 errors	2wANOVA	Normal	37.8 ± 1.43	34.09 ± 1.59	38.29 ± 1.79	2.296	0.111	0.445	0.300	0.586	0.084	5.165	0.009	0.804	−	−	-
Number of type 2 errors	2wANOVA	Nonnormal	4.04 ± 1.11	5.47 ± 1.02	4.51 ± 1.07	0.397	0.674	0.111	4.402	0.041	0.539	3.449	0.039	0.621	−	−	-

Fear conditioning
Training	Test	Data structure				F	p value	Power	WT vs Het	WT vs KO	Het vs KO
- Time	rMeasures	Sph.viol				43.998	0.000	1.000	−	−	−
- Time × genotype	rMeasures	Sph.viol				3.194	0.002	0.970	−	−	−
- Genotype	rMeasures	Sph.viol				14.505	0.000	0.998	0.809	0.000	0.000
- Cohort	rMeasures	Sph.viol				12.351	0.001	0.932	−	−	−
- Time × gen. × coh.	rMeasures	Sph.viol				0.602	0.782	0.281	−	−	−
- Gen. × coh.	rMeasures	Sph.viol				0.494	0.613	0.127	−	−	−

						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
Training, individualtime bins	Test	Data structure	WT	Het	KO	F	pvalue	Power	F	pvalue	Power	F	pvalue	Power	WT vs Het	WT vs KO	Het vsKO
Habituation	2wANOVA	Nonnormal	16.85 ± 2.93	10.45 ± 2.37	21.6 ± 5.3	2.081	0.135	0.408	0.015	0.903	0.052	0.061	0.941	0.059	−	−	-
Pre-tone 0-120	2wANOVA	Nonnormal	10.45 ± 1.72	8.15 ± 1.43	21.01 ± 3.97	6.546	0.003	0.892	0.330	0.568	0.087	0.041	0.960	0.056	0.820	0.021	0.004
Tone/shock 120-140	2wANOVA	Nonnormal	10.58 ± 3.26	6.85 ± 2.82	19.94 ± 6.06	2.361	0.105	0.456	0.020	0.887	0.052	0.199	0.820	0.079	−	−	-
Post-tone 140-260	2wANOVA	Nonnormal	19.74 ± 3.73	18.83 ± 3.86	47.68 ± 6.71	13.149	0.000	0.996	5.506	0.023	0.634	2.222	0.119	0.433	0.990	0.000	0.000
Tone/shock 260-280	2wANOVA	Nonnormal	15.07 ± 4.7	24.58 ± 5.22	47.23 ± 7.47	7.613	0.001	0.934	0.026	0.871	0.053	0.762	0.472	0.173	0.507	0.001	0.027
Tone/shock 260-280	2wANOVA	Nonnormal	31.06 ± 5.14	37.88 ± 6.6	65.23 ± 6.72	12.505	0.000	0.995	18.006	0.000	0.986	0.640	0.532	0.151	0.650	0.000	0.002
Tone/shock 400-420	2wANOVA	Nonnormal	31.03 ± 5.86	40.59 ± 7.61	65.21 ± 5.94	9.728	0.000	0.977	12.565	0.001	0.935	0.061	0.941	0.059	0.503	0.001	0.015
Post-tone 420-540	2wANOVA	Nonnormal	36.71 ± 6.53	47.61 ± 7.36	61.74 ± 6.78	7.880	0.001	0.942	45.207	0.000	1.000	0.135	0.874	0.070	0.303	0.003	0.139

Context	Test	Data structure				F	p value	Power	WT vs Het	WT vs KO	Het vs KO
- Time	rMeasures	Sph.ass				4.558	0.004	0.880	−	−	−
- Time × genotype	rMeasures	Sph.ass				0.675	0.670	0.262	−	−	−
- Genotype	rMeasures	Sph.ass				1.788	0.178	0.357	−	−	−
- Cohort	rMeasures	Sph.ass				0.542	0.465	0.112	−	−	−
- Time × gen.× coh.	rMeasures	Sph.ass				0.918	0.481	0.355	−	−	−
- Gen. × coh.	rMeasures	Sph.ass				1.026	0.366	0.219	−	−	−

						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
Context, individual time bins	Test	Datastructure	WT	Het	KO	F	p value	Power	F	pvalue	Power	F	p value	Power	WT vs Het	WT vs KO	Het vs KO
0-60	2wANOVA	Nonnormal	63.09 ± 5.13	57.19 ± 5.63	44.3 ± 6.21	2.643	0.081	0.502	0.582	0.449	0.116	0.558	0.576	0.137	0.750	0.063	0.261
60-120	2wANOVA	Nonnormal	66.34 ± 6.83	66 ± 6.94	59.6 ± 7.41	0.230	0.795	0.084	0.676	0.415	0.127	2.233	0.118	0.435	−	−	-
120-180	2wANOVA	Nonnormal	62.65 ± 7.14	62.11 ± 6.47	42.81 ± 7.83	2.263	0.114	0.440	0.392	0.534	0.094	0.958	0.390	0.207	−	−	-
180-240	2wANOVA	Nonnormal	56.12 ± 6.56	54.45 ± 7.58	43.33 ± 6.29	0.944	0.396	0.205	0.073	0.788	0.058	0.109	0.897	0.066	−	−	-
mean	2wANOVA	Nonnormal	62.05 ± 5.57	59.94 ± 5.31	47.51 ± 5.84	1.788	0.178	0.357	0.542	0.465	0.112	1.026	0.366	0.219	−	−	-

Cued	Test	Data structure				F	p value	Power	WT vs Het	WT vs KO	Het vs KO
- Time	rMeasures	Sph.viol				25.753	0.000	1.000	−	−	−
- Time × genotype	rMeasures	Sph.viol				3.101	0.002	0.968	−	−	−
- Genotype	rMeasures	Sph.viol				5.657	0.006	0.841	0.645	0.007	0.065
- Cohort	rMeasures	Sph.viol				4.255	0.044	0.525	−	−	−
- Time × gen. × coh.	rMeasures	Sph.viol				4.116	0.000	0.995	−	−	−
- Gen. × coh.	rMeasures	Sph.viol				1.616	0.209	0.326	−	−	−

						Genotype			Cohort			Genotype × cohort			Pairwise comparisons
Cued, individual time bins	Test	Data structure	WT	Het	KO	F	p value	Power	F	p value	Power	F	p value	Power	WT vs Het	WT vs KO	Het vsKO
Pre-tone 0-60	2wANOVA	Nonnormal	1.64 ± 1.14	0.4 ± 0.28	4.9 ± 2.59	1.897	0.160	0.376	5.996	0.018	0.671	1.527	0.227	0.310	0.841	0.311	0.114
Pre-tone 60-120	2wANOVA	Nonnormal	0.84 ± 0.38	1.96 ± 0.89	2.37 ± 0.88	1.056	0.355	0.225	18.576	0.000	0.988	0.747	0.479	0.170	0.461	0.238	0.896
Tone 120-140	2wANOVA	Nonnormal	10.94 ± 4.37	12.14 ± 4.53	25.23 ± 6.38	3.005	0.058	0.558	7.144	0.010	0.746	1.298	0.282	0.268	0.984	0.106	0.150
Post-tone 140-200	2wANOVA	Nonnormal	8.52 ± 2.11	7.41 ± 1.76	13.09 ± 3.74	1.367	0.264	0.281	1.610	0.210	0.238	0.118	0.889	0.067	−	−	-
Post-tone 200-260	2wANOVA	Nonnormal	2.29 ± 1.03	6.59 ± 1.53	10.03 ± 4.8	1.551	0.222	0.314	1.595	0.212	0.236	1.203	0.309	0.251	−	−	-
Tone 260-280	2wANOVA	Nonnormal	13.87 ± 5.15	19.36 ± 5.92	39.08 ± 7.75	7.219	0.002	0.921	15.352	0.000	0.970	7.888	0.001	0.942	0.733	0.003	0.025
Post-tone 280-340	2wANOVA	Nonnormal	8.92 ± 2.71	19.61 ± 5.1	26.7 ± 6.01	3.891	0.027	0.677	5.448	0.024	0.629	1.180	0.316	0.247	0.240	0.024	0.527
Post-tone 340-400	2wANOVA	Nonnormal	5.09 ± 1.34	9.96 ± 2.78	20.99 ± 4.81	6.189	0.004	0.874	1.570	0.216	0.233	1.068	0.351	0.227	0.549	0.003	0.054

WT, wild-type mice; Het, heterozygous mice; KO, homozygous knock-out mice. Group values are reported as mean ± SEM. Bold font indicates significant results (p < 0.05). Individual results and statistical analyses for cohorts 1 and 2 are available in Extended Data Table 12-1. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, one sample t test: 1S-t test, Norm: normal, No-norm: non-normal, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort. 2wANOVA: 2-way ANOVA, rMeasures: repeated measures, one sample t test: 1S-t test, Sph.ass: sphericity assumed, Sph.viol: sphericity violated, gen: genotype, coh: cohort.