Quantification of GFP positive cell numbers
| Genotype | TH GAL4 > UAS CaLexA | Pdfr5304; TH GAL4 > UAS CaLexA | ||
|---|---|---|---|---|
| Time point | ZT4 | ZT14 | ZT4 | ZT14 |
| n (hemispheres) | 14 | 16 | 13 | 12 |
| PAL (13) | 1.4 ± 0.3 | 1.3 ± 0.3 | 2.4 ± 0.4 | 2.1 ± 0.4 |
| PAM (5) | 0.1 ± 0.09 | 0.4 ± 0.2 | 0.1 ± 0.1 | 0 |
| PPM1 (5)a | 0.1 ± 0.09 | 0 | 0 | 0 |
| PPM2 (8) | 2.1 ± 0.5 | 2.6 ± 0.4 | 2 ± 0.5 | 2 ± 0.4 |
| PPM3 (8) | 1.1 ± 0.3 | 1 ± 0.3 | 2. 2 ± 0.4 | 1.2 ± 0.3 |
| PPL1 (12) | 0.3 ± 0.1 | 0.7 ± 0.3 | 0.2 ± 0.1 | 1.5 ± 0.4 |
| PPL2ab (6) | 0.07 ± 0.07 | 0.8 ± 0.3 | 1.1 ± 0.3 | 0.8 ± 0.3 |
| PPL2c (4) | 0 | 0 | 0.08 ± 0.08 | 0.08 ± 0.08 |
| PPL3 (1) | 0.3 ± 0.2 | 0.3 ± 0.2 | 0.3 ± 0.3 | 0.2 ± 0.1 |
| PPL4 (1) | 0 | 0.06 ± 0.06 | 0 | 0 |
Number of GFP+ neurons as seen in different dopaminergic subsets (mean ± SEM) in brain hemispheres expressing CaLexA under the TH GAL4 driver in wild-type and pdfr 5304 backgrounds during day (ZT4) and night (ZT14) time points. Numbers in parentheses indicate overall number of neurons of different subsets per hemisphere that are TH GAL4-positive as seen from data in Mao and Davis (2009). aNumber of neurons per brain.