Compositions of pipette and bath solutions and liquid junction potentials in experiments with HEK293 cells
| NaCl | KCl | CaCl2 | MgCl2 | Na2EGTA | HEPES | Glucose | NaAsp | pH | LJP | |
|---|---|---|---|---|---|---|---|---|---|---|
| Pipette standard | — | 126 | 0.5 | 2 | 5 | 25 | — | — | 7.4 | — |
| Bath standard | 150 | — | 1.8 | 1 | — | 10 | 5 | — | 7.4 | 4.7 |
| Bath Asp | — | — | 1.8 | 1 | — | 10 | 5 | 150 | 7.4 | -7 |
Asp, aspartate; EGTA, ethylene glycol tetraacetic acid; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; LJP, liquid junction potential. All concentrations are in mM.