Table 4.

Statistical summary for Figures 3, 4

Variable		Comparison	Data structure	Statistical test	Output	p value	Post hoc power	Effect size
Sociability investigation time	v	Celf6-Extended, drug (FLX vs vehicle)	Normal	Two-way rmANOVA	F_(1,111) = 5.608	p = 0.020	0.651	0.225
	x	Celf6 ⁺⁺ FLX social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 6.983	p = 0.009	0.745	0.250
	x	Celf6 ^+/- FLX social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 5.440	p = 0.021	0.638	0.222
	x	Celf6 ^-/- FLX social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 7.821	p = 0.006	0.792	0.266
	x	Celf6⁺⁺ vehicle social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 5.998	p = 0.016	0.680	0.232
	x	Celf6 ^+/- vehicle social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 8.852	p = 0.004	0.839	0.283
	x	Celf6^-/- vehicle social vs empty stimulus	Normal	Simple main effect	F_(1,111) = 15.898	p = 0.0001	0.977	0.378
	w	Social stimulus FLX vs vehicle	Normal	Simple main effect	F_(1,222) = 4.895	p = 0.028	0.596	0.150
	y	Long Prenatal, stimulus × drug interaction	Normal	One-way rmANOVA	F_(1,40) = 14.627	p = 0.0004	0.962	0.605
	z	FLX social vs empty stimulus	Normal	Simple main effect	F_(1,40) = 0.216	p = 0.645	0.074	0.071
	aa	Vehicle social vs empty stimulus	Normal	Simple main effect	F_(1,40) = 28.149	p < 0.000005	0.999	0.839
	bb	Social stimulus FLX vs vehicle	Normal	Simple main effect	F_(1,80) = 16.659	p = 0.0001	0.981	0.456
	cc	Short Prenatal, stimulus × drug interaction	Normal	One-way rmANOVA	F_(1,42) = 0.002	p = 0.962	0.050	0.007
	dd	FLX social vs empty stimulus	Normal	Simple main effect	F_(1,42) = 12.337	p = 0.001	0.929	0.032
	ee	Vehicle social vs empty stimulus	Normal	Simple main effect	F_(1,42) = 11.715	p = 0.001	0.917	0.032
	ff	Social stimulus FLX vs vehicle	Normal	Simple main effect	F_(1,84) = 0.124	p = 0.726	0.064	0.032
Social novelty investigation time	gg	Celf6-Extended, drug (FLX vs vehicle)	Normal	Two-way rmANOVA	F_(1,111) = 3.468	p = 0.065	0.455	0.176
	hh	Celf6 ⁺⁺ FLX Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 8.845	p = 0.004	0.838	0.283
	hh	Celf6 ^+/- FLX Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 7.618	p = 0.007	0.781	0.261
	hh	Celf6 ^-/- FLX Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 11.659	p = 0.0009	0.923	0.324
	hh	Celf6 ⁺⁺ vehicle Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 5.812	p = 0.018	0.666	0.229
	hh	Celf6 ^+/- vehicle Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 9.616	p = 0.002	0.867	0.295
	hh	Celf6 ^-/- vehicle Fam vs novel stimulus	Normal	Simple main effect	F_(1,111) = 18.954	p = 0.00003	0.991	0.413
	hh	Long Prenatal, stimulus (Fam vs novel cup)	Non-normal	One-way rmANOVA	F_(1,40) = 46.742	p < 0.000005	1	1.081
	hh	FLX Fam vs novel stimulus	Non-normal	Simple main effect	F_(1,40) = 11.365	p = 0.002	0.908	0.533
	hh	Vehicle Fam vs novel stimulus	Non-normal	Simple main effect	F_(1,40) = 42.911	p < 0.000005	1	1.037
	hh	Short Prenatal, stimulus (Fam vs novel cup)	Non-normal	One-way rmANOVA	F_(1,40) = 13.815	p = 0.001	0.952	0.588
	hh	FLX Fam vs novel stimulus	Non-normal	Simple main effect	F_(1,40) = 10.119	p = 0.003	0.874	0.503
	hh	Vehicle Fam vs novel stimulus	normal	Simple main effect	F_(1,40) = 4.307	p = 0.044	0.526	0.328
Percent tube test bouts won	ii	C57-Extended FLX, compared to 50%	Non-normal	One-spl Wilcoxon	Z = 2.418	p = 0.016	N/A	0.24
	jj	C57-Extended vehicle, compared to 50%	Non-normal	One-spl Wilcoxon	Z = -2.398	p = 0.016	N/A	0.24
	kk	Long Prenatal FLX, compared to 50%	Non-normal	One-spl Wilcoxon	Z = -2.356	p = 0.018	N/A	0.69
	kk	Long Prenatal vehicle, compared to 50%	Non-normal	One-spl Wilcoxon	Z = 1.873	p = 0.061	N/A	0.44
	ll	Short Prenatal FLX, compared to 50%	Non-normal	One-spl Wilcoxon	Z = -1.907	p = 0.057	N/A	0.20
	ll	Short Prenatal vehicle, compared to 50%	Non-normal	One-spl Wilcoxon	Z = 1.691	p = 0.091	N/A	0.16
Adult weight	mm	C57-Extended, drug (FLX vs vehicle)	Normal	Two-way ANOVA	F_(1,12) = 0.475	p = 0.504	0.097	0.199
	nn	Long Prenatal, drug (FLX vs vehicle)	Normal	Two-way ANOVA	F_(1,40) = 8.096	p = 0.007	0.793	0.449
	oo	Short Prenatal, drug (FLX vs vehicle)	Normal	Two-way ANOVA	F_(1,40) = 1.796	p = 0.188	0.258	0.212

Effect sample size for F tests reported as Cohen’s f (Cohen, 1988; interpretation: 0.01 = small; 0.25 = medium; 0.40 = large) and for nonparametric tests reported as η².