Table 2.

Statistical summary for Figures 1, 2

Variable		Comparison	Data structure	Statistical test	Output	p value	Post hoc power	Effect size
Number of USVs	a	Celf6-Extended, drug (FLX vs vehicle)	Non-normal	Two-way rmANOVA	F_(1,197) = 80.854	p < 0.000005	1	0.641
	b	Celf6-Extended, age × drug × genotype interaction	Non-normal	Two-way rmANOVA	F_{(3.66,360.87)} = 2.478	p = 0.049	0.667	0.160
	c	Vehicle at P9 Celf6^+/+ vs Celf6^+/- vs Celf6^-/-	Non-normal	Simple main effect	F_(2,591) = 15.454	p < 0.000005	0.967	0.422
	d	Celf6 ^+/+ at P5 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 5.214	p = 0.023	0.625	0.095
	d	Celf6 ^+/+ at P7 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 24.168	p < 0.000005	0.998	0.201
	d	Celf6 ^+/+ at P9 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 32.669	p < 0.000005	1	0.234
	d	Celf6 ^+/- at P5 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 8.307	p = 0.004	0.821	0.119
	d	Celf6 ^+/- at P7 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 53.427	p < 0.000005	1	0.301
	d	Celf6 ^+/- at P9 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 35.638	p < 0.000005	1	0.246
	d	Celf6 ^-/- at P5 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 2.724	p = 0.099	0.378	0.071
	d	Celf6 ^-/- at P7 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 24.936	p < 0.000005	0.999	0.204
	d	Celf6 ^-/- at P9 FLX vs vehicle	Non-normal	Simple main effect	F_(1,591) = 1.380	p = 0.241	0.217	0.045
	g	Long Prenatal, drug (FLX vs vehicle)	Non-normal	One-way rmANOVA	F_(1,43) = 18.013	p = 0.0001	0.986	0.647
	h	P5 FLX vs vehicle	Non-normal	Simple main effect	F_(1,43) = 14.689	p = 0.0004	0.963	0.585
	h	P7 FLX vs vehicle	Non-normal	Simple main effect	F_(1,43) = 16.678	p = 0.0002	0.979	0.622
	i	P9 FLX vs vehicle	Non-normal	Simple main effect	F_(1,43) = 3.874	p = 0.056	0.486	0.301
	k	Short Prenatal, drug (FLX vs vehicle)	Non-normal	One-way rmANOVA	F_(1,43) = 0.041	p = 0.840	0.052	<0.000
Average duration	e	Celf6-Extended, drug (FLX vs vehicle)	Normal	Linear mixed model	F_(1,211.820) = 31.223	p < 0.000005	[0.005, 0.010]
Simple call pitch range	f	Celf6-Extended, drug (FLX vs vehicle)	Normal	Linear mixed model	F_(1,170.380) = 38.155	p < 0.000005	[1895.15, 3675.32]
Simple call pitch range	j	Long Prenatal, drug (FLX vs vehicle)	Normal	Linear mixed model	F_(1,44.068) = 5.256	p = 0.027	[251.10, 3901.71]
Weight	k	Celf6-Extended, age (P5 vs P7 vs P9 vs P14)	Normal	Two-way rmANOVA	F_(1.46,286.7) = 2670.61	p < 0.000005	1	3.673
	m	Celf6-Extended, drug (FLX vs vehicle)	Normal	Two-way rmANOVA	F_(1,197) = 56.921	p < 0.000005	1	0.537
	n	Celf6 ^+/+ at P5 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 8.087	p = 0.005	0.811	0.101
	n	Celf6 ^+/+ at P7 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 8.008	p = 0.005	0.807	0.101
	n	Celf6 ^+/+ at P9 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 13.699	p = 0.0003	0.959	0.132
	n	Celf6 ^+/+ at P14 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 34.952	p < 0.000005	1	0.209
	n	Celf6 ^+/- at P5 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 14.860	p = 0.0001	0.971	0.139
	n	Celf6 ^+/- at P7 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 18.036	p = 0.00002	0.989	0.150
	n	Celf6 ^+/- at P9 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 21.454	p < 0.000005	0.996	0.167
	n	Celf6 ^+/- at P14 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 42.427	p < 0.000005	1	0.232
	n	Celf6 ^-/- at P5 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 7.462	p = 0.006	0.779	0.095
	n	Celf6 ^-/- at P7 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 8.869	p = 0.003	0.845	0.105
	n	Celf6 ^-/- at P9 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 12.822	p = 0.0004	0.947	0.128
	n	Celf6 ^-/- at P14 FLX vs vehicle	Normal	Simple main effect	F_(1,788) = 18.815	p = 0.00002	0.991	0.153
	q	Celf6-Extended, litter (FLX vs vehicle)	Non-normal	Mann–Whitney U	U₍₂₀₃₎ = 4723.5	p = 0.301	N/A	0.01
	k	Long Prenatal, age (P5 vs P7 vs P9 vs P14)	Normal	Two-way rmANOVA	F_(2.26,97.31) = 1231.23	p < 0.000005	1	5.330
	m	Long Prenatal, drug (FLX vs vehicle)	Normal	Two-way rmANOVA	F_(1,43) = 20.887	p = 0.00004	0.994	0.697
	n	P5 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 4.163	p = 0.043	0.528	0.157
	n	P7 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 12.029	p = 0.0007	0.932	0.264
	n	P9 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 27.769	p < 0.000005	0.999	0.402
	n	P14 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 31.829	p < 0.000005	1	0.430
	q	Long Prenatal, litter (FLX vs vehicle)	Non-normal	Mann–Whitney U	U₍₄₅₎ = 228	p = 0.595	N/A	0.01
	k	Short Prenatal, age (P5 vs P7 vs P9 vs P14)	Normal	Two-way rmANOVA	F_(1.64,70.58) = 892.959	p < 0.000005	0.954	4.554
	o	Short Prenatal, drug (FLX vs vehicle)	Normal	Two-way rmANOVA	F_(1,43) = 25.719	p = 0.000008	0.999	0.773
	p	P5 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 5.273	p = 0.023	0.627	0.176
	p	P7 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 13.753	p = 0.0003	0.958	0.283
	p	P9 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 19.138	p = 0.00002	0.992	0.333
	p	P14 FLX vs vehicle	Normal	Simple main effect	F_(1,172) = 49.019	p < 0.000005	1	0.534
	r	Short Prenatal, litter (FLX vs vehicle)	Non-normal	Mann–Whitney U test	U₍₄₅₎ = 84.5	p = 0.00003	N/A	0.35
Latency to righting reflex	s	Celf6-Extended, drug (FLX vs vehicle)	Non-normal	Two-way ANOVA	F_(1,191) = 13.753	p = 0.004	0.827	0.212
	t	Long Prenatal, drug (FLX vs vehicle)	Non-normal	Mann–Whitney U	U₍₄₅₎ = 223.0	p = 0.545	N/A	0.01
	u	Short Prenatal, drug (FLX vs vehicle)	Non-normal	Mann–Whitney U	U₍₄₅₎ = 187.5	p = 0.140	N/A	0.05

Effect size for F tests reported as Cohen’s f (Cohen, 1988; interpretation: 0.01 = small; 0.25 = medium; 0.40 = large) and for nonparametric tests reported as η². 95% confidence intervals reported for linear mixed models.