Summary of results gained by analysis of fluorescence staining after SE
| Marker | Target | Colocalization with FITC-albumin? | Altered immunosignal in FITC-albumin-positive vs. negative areas? |
|---|---|---|---|
| NeuN | Neurons | (Yes)* | n.d. |
| GFAP | Astroglia | No | Reduced |
| Iba1 | Microglia | Yes | n.d. |
| STL | Vasculature/endothelial cells, perivascular microglia | No | Reduced |
| Laminin | Vascular basement membranes of endothelium | Yes | No |
| Collagen IV | Vascular basement membranes of endothelium | No | Elevated |
| AQP4 | Astroglial endfeet | No | Reduced |
| S100β | Astroglial somata | No | No |
| CD45c | Activated microglia | No | Reduced |
| CD8b | Subset of T cells | Yes | No |
AQP4, aquaporin-4; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1; n.d., not determined; NeuN, neuronal nuclei; STL, Solanum tuberosum lectin.
*But predominantly in NeuN-immunonegative cells displaying neuronal morphology.