Parameters of eEPSCs in SCN neurons
| Mouse genotype | WT | Controls | cKO | p value |
|---|---|---|---|---|
| Number of mice | 16 | 9 | 12 | |
| Neurons/eEPSC | 19/475 | 20/500 | 16/377 | |
| Peak amplitude (pa) | 156.3 187.8 225.7 | 139.7 181.4 235.6 | 143.2 170.6 203.3 | 0.756 |
| Time to peak (ms) | 4.6 5.1 5.5 | 4.5 4.8 5.1 | 4.4 4.9 5.5 | 0.636 |
| Rise time (ms) | 1.3 1.53 1.7 | 1.3 1.5 1.8 | 1.2 1.5 1.9 | 0.815 |
| Decay time constant (ms) | 5.1 6.4 7.6 | 4.8 5.4 6.0 | 4.0 4.9 5.8 | 0.173 |
| Threshold (V) | 7.6 9.0 10.4 | 6.9 8.8 10.7 | 6.8 8.0 9.3 | 0.569 |
| Stimulation (V) | 12.1 13.5 14.9 | 11.8 14.3 16.9 | 14.4 16.7 18.9 | 0.065 |
WT: wild-type controls; controls: littermate controls; cKO: Opn4Cre/+; Vglut2loxP/loxP. LL and UL CI: lower and upper limits of confidence interval; GEE was used to calculate means, confidence intervals, and p values (shown for comparison between all three mouse types). The center value indicates the mean. The subscripted values indicate the lower (left) and upper (right) limits of the 95% confidence interval. EPSCs were evoked by 0.08-Hz stimulation of the optic chiasm, which did not induce synaptic depression.