Time to shoal was found to be significantly longer in the mTBI versus control group
| Sample | Pre-injury | 0 dpi | 1 dpi | 2 dpi | 3 dpi | p-value |
|---|---|---|---|---|---|---|
| Control | 9.9 ± 3.949 | 13.405 ± 5.429* | 11.223 ± 2.753* | 8.535 ± 2.534 | 6.62 ± 3.37* | |
| mTBI | 9.9 ± 3.949 | 107.488 ± 63.267* | 48.378 ± 29.949* | 17.82 ± 11.197 | 15.97 ± 6.65* |
Spatial memory testing was conducted 1 h after injury on the day of mTBI induction (day 0) as well as once per day for 3 d after the administration of mTBI and sham injuries. Average time to shoal measured in seconds for mTBI and control zebrafish found a statically significant difference between mTBI and sham controls, although no significant effect was found between days or among the interaction effect, p = 0.034, F(1,5) = 8.324. Tukey’s multiple comparison post hoc test confirmed that days 0, 1, and 3 dpi were significantly different between groups (A). n = 6 per group.
*Significant difference between mTBI and control groups on that specific day by Tukey multiple comparison post hoc analysis (p < 0.05).