Raw electrophysiological data
| Genotype | Condition | mEPSP (mV) | mEPSP noise (µV) | mEPSP/noise ratio | mEPSP frequency (Hz) | EPSP (mV) | QC | IR (MΩ) | RMP (mV) | n | Figure |
|---|---|---|---|---|---|---|---|---|---|---|---|
| w1118 (WT) | 25°C | 0.85 ± 0.04 | 16.5 ± 1.8 | 57.3 ± 5.7 | 3.5 ± 0.3 | 32.0 ± 1.2 | 38.5 ± 1.7 | 6.4 ± 0.6 | –64.6 ± 1.1 | 13 | 1, 2 |
| w;; MHC-Gal4/+ | 25°C | 0.65 ± 0.03 | 23.3 ± 1.2 | 28.2 ± 1.3 | 4.3 ± 0.5 | 33.6 ± 1.8 | 52.1 ± 1.8 | 4.8 ± 0.2 | –66.1 ± 1.2 | 13 | 1, 2 |
| UAS-GluRIIAM614R/+; MHC-Gal4/+ | 25°C | 0.44 ± 0.02 | 13.8 ± 0.5 | 32.2 ± 1.7 | 0.6 ± 0.1 | 35.0 ± 1.8 | 80.3 ± 4.1 | 5.8 ± 0.5 | –69.1 ± 1.2 | 11 | 1, 2 |
| w1118 | 29°C | 0.72 ± 0.03 | 16.7 ± 0.7 | 43.6 ± 2.2 | 1.8 ± 0.2 | 32.9 ± 1.2 | 46.4 ± 2.2 | 6.2 ± 0.5 | –64.4 ± 0.9 | 14 | 1, 2 |
| w;; MHC-Gal4/+ | 29°C | 0.52 ± 0.02 | 21.8 ± 0.7 | 24.1 ± 1.0 | 2.4 ± 0.1 | 31.7 ± 1.3 | 61.2 ± 2.4 | 5.3 ± 0.4 | –66.5 ± 1.0 | 12 | 1, 2 |
| UAS-GluRIIAM614R/+; MHC-Gal4/+ | 29°C | 0.35 ± 0.02 | 13.8 ± 0.9 | 26.4 ± 2.3 | 0.2 ± 0.0 | 23.3 ± 1.2 | 68.9 ± 5.7 | 5.2 ± 0.2 | –65.0 ± 1.1 | 10 | 1, 2 |
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | Early third instar; 25°C | 1.27 ± 0.1 | 26.6 ± 3.1 | 53.4 ± 7.3 | 2.0 ± 0.3 | 33.2 ± 2.1 | 27.4 ± 2.4 | 9.5 ± 0.8 | –64.7 ± 2.0 | 10 | 3 |
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | Early third instar; 29°C | 0.60 ± 0.05 | 18.7 ± 1.9 | 32.8 ± 1.2 | 0.4 ± 0.1 | 25.1 ± 1.4 | 43.1 ± 2.6 | 13.6 ± 0.9 | –63.0 ± 0.8 | 10 | 3 |
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | 21°C | 0.83 ± 0.03 | 5.1 ± 0.5 | 33.3 ± 1.9 | 40.7 ± 2.4 | 5.6 ± 0.4 | –66.3 ± 0.8 | 14 | 4, 5 | ||
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | 29°C | 0.42 ± 0.02 | 0.4 ± 0.1 | 25.6 ± 2.1 | 62.5 ± 4.8 | 6.0 ± 0.3 | –66.8 ± 1.1 | 15 | 4, 5 | ||
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | 1-d recovery at 21°C | 0.39 ± 0.02 | 0.6 ± 0.1 | 24.1 ± 1.4 | 63.1 ± 5.0 | 5.3 ± 0.3 | –66.0 ± 1.1 | 9 | 4, 5 | ||
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | 2-d recovery at 21°C | 0.69 ± 0.02 | 3.4 ± 0.3 | 37.8 ± 1.2 | 55.4 ± 1.9 | 5.3 ± 0.3 | –67.7 ± 0.7 | 10 | 4, 5 | ||
| UAS-GluRIIAM614R/+; MHC-Gal4/Gal80TS | 3-d recovery at 21°C | 0.80 ± 0.04 | 5.6 ± 0.4 | 35.3 ± 1.5 | 44.7 ± 1.7 | 5.5 ± 0.2 | –68.4 ± 0.9 | 11 | 4, 5 | ||
| UAS-GluRIIAM614R/+; BG57-Gal4/Gal80TS | 25°C | 0.79 ± 0.04 | 2.4 ± 0.7 | 31.1 ± 1.6 | 39.5 ± 1.9 | 5.3 ± 0.4 | –68.4 ± 1.1 | 13 | 6 | ||
| UAS-GluRIIAM614R/+; BG57-Gal4/Gal80TS | 28.5°C | 0.62 ± 0.05 | 0.6 ± 0.1 | 31.5 ± 1.3 | 53.6 ± 3.6 | 7.2 ± 0.4 | –67.0 ± 1.3 | 13 | 6 | ||
| UAS-GluRIIAM614R/+; BG57-Gal4/Gal80TS | 2-d recovery at 25°C | 0.79 ± 0.03 | 1.4 ± 0.2 | 31.3 ± 1.4 | 39.9 ± 1.8 | 6.3 ± 0.3 | –66.7 ± 1.1 | 14 | 6 | ||
| w1118 | 30°C | 0.78 ± 0.04 | 2.4 ± 0.2 | 33.1 ± 1.4 | 42.9 ± 1.9 | 6.8 ± 0.3 | –66.2 ± 0.8 | 15 | 7 | ||
| w; GluRIIASP16 | 30°C | 0.32 ± 0.02 | 0.8 ± 0.1 | 21.9 ± 2.2 | 67.4 ± 6.8 | 6.0 ± 0.4 | –63.0 ± 0.5 | 15 | 7 | ||
| MHC-Gal4/+ | 30°C | 0.47 ± 0.02 | 1.1 ± 0.1 | 28.9 ± 0.6 | 64.3 ± 3.6 | 4.9 ± 0.2 | –63.9 ± 0.9 | 15 | 7 | ||
| UAS-GluRIIAM614R/+; MHC-Gal4/+ | 30°C | 0.33 ± 0.01 | 0.2 ± 0.0 | 15.4 ± 1.1 | 47.0 ± 2.8 | 5.3 ± 0.2 | –64.4 ± 0.9 | 13 | 7 | ||
| w;; BG57-Gal4/+ | 30°C | 0.51 ± 0.03 | 0.6 ± 0.1 | 31.3 ± 1.4 | 63.9 ± 4.5 | 6.6 ± 0.3 | –70.2 ± 1.4 | 12 | 7 | ||
| UAS-GluRIIAM614R/+; BG57-Gal4/+ | 30°C | 0.35 ± 0.03 | 0.8 ± 0.7 | 13.5 ± 1.3 | 39.1 ± 3.4 | 6.1 ± 0.5 | –65.5 ± 1.6 | 9 | 7 | ||
| w1118 | 31°C | 0.71 ± 0.03 | 2.4 ± 0.2 | 36.9 ± 1.6 | 53.6 ± 3.0 | 2.0 ± 0.2 | –68.1 ± 1.5 | 14 | 7 | ||
| w; GluRIIASP16 | 31°C | 0.36 ± 0.02 | 1.1 ± 0.2 | 25.8 ± 1.5 | 74.4 ± 5.4 | 4.2 ± 0.9 | –66.6 ± 1.3 | 13 | 7 | ||
| w1118 | 32°C | 0.47 ± 0.01 | 2.4 ± 1.1 | 30.7 ± 1.9 | 64.8 ± 4.1 | 2.0 ± 0.4 | –63.7 ± 0.9 | 13 | 7 | ||
| w; GluRIIASP16 | 32°C | 0.37 ± 0.03 | 1.4 ± 0.9 | 21.0 ± 2.2 | 56.1 ± 4.4 | 5.6 ± 0.3 | –65.0 ± 0.8 | 10 | 7 |
Full genotypes and rearing conditions for electrophysiological data presented in the study. Average values ± SEM are presented for each parameter; n, number of NMJs recorded. Values include mEPSP amplitude, noise, amplitude to noise ratio, and frequency; EPSP amplitude; quantal content (QC); muscle input resistance (IR); and resting membrane potential (RMP).