Panel of 48 genes used to probe the neurochemical profile of Hcrt/Ox and MCH neurons
| Gene name/common protein abbreviation | Gene symbol | Function | TaqMan primer sequence |
|---|---|---|---|
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Gapdh | Housekeeping | Mm03302249_g1 |
| Hypoxanthine guanine phosphoribosyl transferase (HPRT) | Hprt | Housekeeping | Mm03024075_m1 |
| Neuronal nuclei (NeuN) | Rbfox3 | Neuronal marker | Mm01248781_m1 |
| Microtubule associated protein 2 (MAP2) | Map2 | Neuronal marker | Mm00485231_m1 |
| Tubulin beta 3 (TuJ1) | Tubb3 | Neuronal marker | Mm00727586_s1 |
| Glial fibrillary acidic protein (GFAP) | Gfap | Glial marker | Mm01253033_m1 |
| S100 calcium binding protein B (S100β) | S100b | Glial marker | Mm00485897_m1 |
| Hypocretin/orexin (Hcrt/Ox) | Hcrt | Neuropeptide | Mm01964030_s1 |
| Pro-melanin-concentrating hormone (MCH) | Pmch | Neuropeptide | Mm01242886_g1 |
| Prodynorphin (DYN) | Pdyn | Neuropeptide | Mm00457573_m1 |
| Galanin (GAL) | Gal | Neuropeptide | Mm00439056_m1 |
| Neurotensin (NTS) | Nts | Neuropeptide | Mm00481140_m1 |
| Nucleobindin 2 (Nesfatin-1 or NUCB2) | Nucb2 | Neuropeptide | Mm01137144_m1 |
| Cocaine and amphetamine regulatory transcript (CART) | Cartpt | Neuropeptide | Mm04210469_m1 |
| Thyrotropin-releasing hormone (TRH) | Trh | Neuropeptide | Mm01182425_g1 |
| Prepronociceptin/orphanin FQ (N/OFQ) | Pnoc | Neuropeptide | Mm01314909_m1 |
| Corticotropin-releasing hormone (CRF) | Crh | Neuropeptide | Mm01293920_s1 |
| Proenkephalin (PENK) | Penk | Neuropeptide | Mm01212875_m1 |
| Substance P (SubP) | Tac1 | Neuropeptide | Mm01166996_m1 |
| Tachykinin2 (TAC2) | Tac2 | Neuropeptide | Mm01160362_m1 |
| Somatostatin (SST) | Sst | Neuropeptide | Mm00436671_m1 |
| Glutamate decarboxylase 2 (GAD65) | Gad2 | GABA synthesis | Mm00484623_m1 |
| Glutamate decarboxylase 1 (GAD67) | Gad1 | GABA synthesis | Mm04207432_g1 |
| Vesicular GABA transporter (VGAT) | Slc32a1 | GABA release | Mm00494138_m1 |
| Vesicular glutamate transporter 1 (VGLUT1) | Slc17a7 | Glutamate release | Mm00436577_m1 |
| Vesicular glutamate transporter 2 (VGLUT2) | Slc17a6 | Glutamate release | Mm00499876_m1 |
| Vesicular glutamate transporter 3 (VGLUT3) | Slc17a8 | Glutamate release | Mm00805413_m1 |
| Vesicle Monoamine Transporter 1 (VMAT1) | Slc18a1 | Monoamine release | Mm00461868_m1 |
| Vesicle Monoamine Transporter 2 (VMAT2) | Slc18a2 | Monoamine release | Mm00553058_m1 |
| Glutaminase (GLS) | Gls | Glutamate synthesis | Mm01257297_m1 |
| Choline acetyltransferase (ChAT) | Chat | Acetylcholine synthesis | Mm01221880_m1 |
| Tyrosine hydroxylase (TH) | Th | Catecholamine synthesis | Mm00447557_m1 |
| Histidine decarboxylase (HDC) | Hdc | Histamine synthesis | Mm00456104_m1 |
| Tryptophan hydroxylase 1 (TPH1) | Tph1 | Serotonin synthesis | Mm01202614_m1 |
| Tryptophan hydroxylase 2 (TPH2) | Tph2 | Serotonin synthesis | Mm00557715_m1 |
| Adenosine deaminase (ADA) | Ada | Adenosine metabolism | Mm00545720_m1 |
| Parvalbumin (PV) | Pvalb | Calcium-binding protein | Mm00443100_m1 |
| Calretinin (CR) | Calb2 | Calcium-binding protein | Mm00801461_m1 |
| Calbindin (CALB) | Calb1 | Calcium-binding protein | Mm00486647_m1 |
| NK2 homeobox 1 (NKX2.1) | Nkx2-1 | Transcription factor | Mm00447558_m1 |
| LIM homeobox 9 (LHX9) | Lhx9 | Transcription factor | Mm00495308_m1 |
| Neuronal pentraxin 2 (NPTX2) | Nptx2 | Synaptic function | Mm00479438_m1 |
| Insulin-like growth factor binding protein 3 (IGFBP3) | Igfbp3 | Growth factor binding | Mm01187817_m1 |
| CUGBP, Elav-like family member 6 (CELF6) | Celf6 | RNA binding | Mm01176134_m1 |
| Neuronatin (NNAT) | Nnat | Ion channel function | Mm00731416_s1 |
| Leptin receptor (LEPR) | Lepr | Receptor | Mm00440181_m1 |
| Ghrelin receptor (GHSR) | Ghsr | Receptor | Mm00616415_m1 |
| Melanocortin 4 receptor (MC4-R) | Mc4r | Receptor | Mm00457483_s1 |