Statistical analyses
| Data structure | Type of test | CI | Data structure | Type of test | CI | ||
|---|---|---|---|---|---|---|---|
| Table 2, row 1; Fig. 1C | Automated quantification of Fos+ cells in CG1 from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA with significant main effect of group followed by Fishers LSD n = 5-7 *p < 0.05 | CTRL: (119.9-193.3) primary: (147.1-294.4) bystander: (195.9-308.0) | Table 2, row 14 | Manual quantification of Fos+ cells in posteromedial cortical amygdaloid from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (5.040-14.10) primary: (4.533-10.61) bystander: (7.170-10.27) |
| Table 2, row 2 | Automated quantification of Fos+ cells in CG2 from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-7 p > 0.05 | CTRL: (62.64-86.59) primary: (23.61-126.7) bystander: 41.03-113.6) | Table 2, row 15 | Manual quantification of Fos+ cells in basolateral amygdala from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (6.156-19.07) primary: (5.469-18.79) bystander: (5.186-30.38) |
| Table 2, row 3 | Automated quantification of Fos+ cells in GI from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-7 p > 0.05 | CTRL: (12.35-25.82) primary: (16.69-30.81) bystander: (8.518-40.40) | Table 2, row 16 | Manual quantification of Fos+ cells in central nucleus of the amygdala from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (5.992-12.13) primary: (2.869-8.797) bystander: (3.828-16.21) |
| Table 2, row 4; Fig. 1D | Automated quantification of Fos+ cells in INS from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA with significant main effect of group followed by Fishers LSD n = 5-6 *p < 0.05 | CTRL: (25.97-45.22) primary: (27.64-74.08) bystander: (29.72-116.3) | Table 2, row 17 | Manual quantification of Fos+ cells in paraventricular nucleus from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (1.755-8.645) primary: (7.872-12.30) bystander: (0.972-25.83) |
| Table 2, row 5 | Automated quantification of Fos+ cells in S1 from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (432.5-651.8) primary: (191.4-1128) bystander: (262.4-636.9) | Table 2, row 18 | Quantification of Fos+ cells in the DMH from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA with significant main effect followed by Fishers LSD n = 6-7 **p = 0.007 | CTRL: (10.64-21.66) primary: (17.37-43.81) bystander: (15.25-26.22) |
| Table 2, row 6 | Manual quantification of Fos+ cells in dorsal lateral septum from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (3.120-10.67) primary: (1.763-12.15) bystander: (3.195-9.805) | Table 2, row 19 | Manual quantification of Fos+ cells in the centrally projecting Edinger Westphal from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (4.866-11.11) primary: (4.261-20.06) bystander: (6.974-15.78) |
| Table 2, row 7 | Manual quantification of Fos+ cells in intermediate lateral septum from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (18.13-29.51) primary: (12.99-25.89) bystander: (20.04-27.38) | Table 2, row 20 | Manual quantification of Fos+ cells in periaqueductal gray from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (27.73-62.22) primary: (30.75-129.2) bystander: (42.31-85.50) |
| Table 2, row 8 | Manual quantification of Fos+ cells in ventral lateral septum from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (11.23-16.07) primary: (5.770-22.95) bystander: (5.559-18.98) | Table 2, row 21 | Manual quantification of Fos+ cells in substantia nigra from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (-0.945-10.30) primary: (1.372-14.46) bystander: (2.184-10.03) |
| Table 2, row 9 | Manual quantification of Fos+ cells in nucleus accumbens from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (5.400-37.91) primary: (10.44-41.08) bystander: (15.03-31.01) | Table 2, row 22 | Manual quantification of Fos+ cells in ventral tegmental Area from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (-1.562-8.729) primary: (-4.473-20.07) bystander: (-9.419-31.42) |
| Table 2, row 10 | Manual quantification of Fos+ cells in anterior bed nucleus of the stria terminalis from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (25.51-36.28) primary: (19.48-76.52) bystander: (25.76-52.53) | Fig. 1B | Comparison of mechanical thresholds of each group over 3 test sessions | Two-way RM ANOVA, significant for test session n = 6-7 *p < 0.05 | CTRL: (1.521-2.143) primary: (-0.255-2.787) bystander: (0.031-2.950) |
| Table 2, row 11 | Manual quantification of Fos+ cells in posterior bed nucleus of the stria terminalis from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (-1.066-17.96) primary: (0.4374-5.382) bystander: (1.829-7.948) | Fig. 2F | Comparison of mechanical thresholds of each group following treatment with Veh or CNO on the second test session (inhibition of the ACC) | Two-way ANOVA, significant main effect of treatment and significant interaction followed by Fishers LSD n = 5-7 *p < 0.05 | CTRL/Veh: (0.76-1.68) CTRL/CNO: (0.70-1.54) bystander/VEH: (0.31-0.76) bystander/CNO: (0.39-1.85) primary/Veh: (0.16-0.65) primary/CNO:(0.36-2.14) |
| Table 2, row 12 | Manual quantification of Fos+ cells in dentate gyrus from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (24.09-29.40) primary: (22.46-55.44) bystander: (28.60-38.16) | Fig. 2G | Comparison of mechanical thresholds of each group following treatment with Veh or CNO on the second test session (inhibition of S1) | Two-way ANOVA, significant main effect of group, no interaction n = 5-7 *p < 0.0001 | CTRL/Veh: (-7.73-11.46) CTRL/CNO: (0.368-2.32) bystander/VEH: (-0.088-1.23) bystander/CNO: (-4.63-6.18) primary/Veh: (-0.62-1.91) primary/CNO:(-0.80-3.36) |
| Table 2, row 13 | Manual quantification of Fos+ cells in posterolateral cortical amygdaloid from two separate reactions. Counts were averaged between the 2 runs, with 2-4 slices for each region per mouse per batch (average of 4-8 slices per mouse) | One-way ANOVA n = 5-6 p > 0.05 | CTRL: (18.99-30.39) primary: (8.949-25.65) bystander: (13.75-19.45) |