Table 4.

Summary of the results of the behavioral outcomes used for phenotype analysis in APP^Swe mice

Behavior	Test	Dependent variable	Genotype	Treatment	Mean ± SEM	p value	Interpretation
Locomotion	AC	Ambulatory distance (cm)	APP^Swe nTg	Vehicle	108.20 ± 9.45	—	APP^Swe Tg mice exhibit locomotor hyperactivity
			APP^Swe nTg	ISRIB	88.75 ± 6.40	ns
			APP^Swe Tg	Vehicle	124.80 ± 9.78	ns
			APP^Swe Tg	ISRIB	139.50 ± 15.10	ns
		Center duration (s)	APP^Swe nTg	Vehicle	90.97 ± 12.30	—
			APP^Swe nTg	ISRIB	83.92 ± 13.13	ns
			APP^Swe Tg	Vehicle	173.40 ± 13.15	p < 0.01
			APP^Swe Tg	ISRIB	154.50 ± 21.89	p < 0.05
Fear-associated learning	Baseline	Freezing (%)	APP^Swe nTg	Vehicle	0.38 ± 0.23	—	APP^Swe Tg mice display impairments in fear-associated learning
			APP^Swe nTg	ISRIB	2.82 ± 2.34	ns
			APP^Swe Tg	Vehicle	1.50 ± 1.26	ns
			APP^Swe Tg	ISRIB	0.57 ± 0.37	ns
	Tone 1		APP^Swe nTg	Vehicle	5.69 ± 2.33	—
			APP^Swe nTg	ISRIB	8.43 ± 4.31	ns
			APP^Swe Tg	Vehicle	2.72 ± 1.61	ns
			APP^Swe Tg	ISRIB	1.90 ± 1.39	ns
	ITI1		APP^Swe nTg	Vehicle	22.83 ± 6.21	—
			APP^Swe nTg	ISRIB	24.18 ± 4.67	ns
			APP^Swe Tg	Vehicle	11.62 ± 5.76	ns
			APP^Swe Tg	ISRIB	8.79 ± 3.27	ns
	Tone 2		APP^Swe nTg	Vehicle	30.36 + 8.32	—
			APP^Swe nTg	ISRIB	39.02 ± 8.62	ns
			APP^Swe Tg	Vehicle	17.13 ± 5.71	ns
			APP^Swe Tg	ISRIB	14.15 ± 4.93	ns
	ITI2		APP^Swe nTg	Vehicle	52.64 ± 6.80	—
			APP^Swe nTg	ISRIB	60.00 ± 5.69	ns
			APP^Swe Tg	Vehicle	18.26 ± 3.56	p < 0.0001
			APP^Swe Tg	ISRIB	12.42 ± 3.62	p < 0.0001
Fear-based retrieval	Context based		APP^Swe nTg	Vehicle	49.45 ± 6.35	—	APPSwe Tg exhibit impaired context-based fear memory retrieval
			APP^Swe nTg	ISRIB	50.76 ± 5.95	ns
			APP^Swe Tg	Vehicle	25.93 ± 3.63	p < 0.01
			APP^Swe Tg	ISRIB	20.10 ± 4.03	p < 0.001
	Cue based		APP^Swe nTg	Vehicle	50.12 ± 10.06	—	APP^Swe Tg mice do not display impairments in cue-based fear memory retrieval
			APP^Swe nTg	ISRIB	56.82 ± 7.39	ns
			APP^Swe Tg	Vehicle	32.64 ± 5.32	ns
			APP^Swe Tg	ISRIB	34.35 ± 6.74	ns
Spatial acquisition learning	MWM D1	Escape latency (s)	APP^Swe nTg	Vehicle	33.10 ± 4.88	ns	APP^Swe Tg do not exhibit impairments in spatial memory acquisition
			APP^Swe nTg	ISRIB	16.08 ± 3.15	ns
			APP^Swe Tg	Vehicle	32.56 ± 5.84	ns
			APP^Swe Tg	ISRIB	24.57 ± 6.55	ns
	MWM D2		APP^Swe nTg	Vehicle	31.44 ± 5.30	ns
			APP^Swe nTg	ISRIB	12.66 ± 2.79	ns
			APP^Swe Tg	Vehicle	26.21 ± 5.01	ns
			APP^Swe Tg	ISRIB	29.79 ± 6.50	ns
	MWM D3		APP^Swe nTg	Vehicle	23.02 ± 3.74	ns
			APP^Swe nTg	ISRIB	9.58 ± 1.93	ns
			APP^Swe Tg	Vehicle	19.19 ± 2.90	ns
			APP^Swe Tg	ISRIB	19.92 ± 3.92	ns
	MWM D4		APP^Swe nTg	Vehicle	25.95 ± 4.86	ns
			APP^Swe nTg	ISRIB	6.91 ± 1.21	ns
			APP^Swe Tg	Vehicle	21.12 ± 3.41	ns
			APP^Swe Tg	ISRIB	14.86 ± 1.82	ns
	MWM D5		APP^Swe nTg	Vehicle	16.56 ± 3.15	p < 0.01
			APP^Swe nTg	ISRIB	19.04 ± 7.50	ns
			APP^Swe Tg	Vehicle	17.29 ± 2.99	ns
			APP^Swe Tg	ISRIB	12.30 ± 1.49	ns
	MWM R		APP^Swe nTg	Vehicle	66.35 ± 7.55	p < 0.0001
			APP^Swe nTg	ISRIB	37.79 ± 9.25	ns
			APP^Swe Tg	Vehicle	71.21 ± 6.16	p < 0.0001
			APP^Swe Tg	ISRIB	58.59 ± 8.69	p < 0.0001
Spatial memory recall	Probe test	Duration (%)	APP^Swe nTg	Vehicle	−13.65 ± 4.63	p < 0.05	APP^Swe Tg display intact spatial memory recall
			APP^Swe nTg	ISRIB	−27.80 ± 7.17	p < 0.05
			APP^Swe Tg	Vehicle	−18.06 ± 5.72	p < 0.05
			APP^Swe Tg	ISRIB	−18.74 ± 6.18	p < 0.05
Working memory	Y-maze	Alternation (%)	APP^Swe nTg	Vehicle	62.17 ± 1.71	p < 0.0001	APP^Swe Tg mice display impaired working memory
			APP^Swe nTg	ISRIB	56.77 ± 3.52	ns
			APP^Swe Tg	Vehicle	56.23 ± 3.71	ns
			APP^Swe Tg	ISRIB	55.46 ± 3.61	ns
Recognition memory	NOR	Duration (%)	APP^Swe nTg	Vehicle	−28.39 ± 10.47	p < 0.05	APP^Swe Tg exhibit deficits in recognition memory
			APP^Swe nTg	ISRIB	1.95 ± 19.43	ns
			APP^Swe Tg	Vehicle	9.34 ± 13.48	ns
			APP^Swe Tg	ISRIB	7.32 ± 12.88	ns

Comparisons made to nTg vehicle control. AC, activity chamber; ITI, intertrial interval; D1-5, day 1-5; R, reversal trial.